ets1-a (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ets1-a
Gene Name:  c-ets-1a proto-oncogene
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATTCATCAGTGTCTGGTTTAACATC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAAGCTGCGCTAGATCTCAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716724
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1314nts
Open reading frame: 1 to 1314

ATGAAAGCTGCGCTAGATCTCAAACCGACATTAAGCATCAAAACAGAGAAAGAGTATGAA
GCGTATTCGTGCTCGGATATGGAATGTGCAGATGTCCCGCTTCTGACACCCAGTAGTAAA
GAGATGATGTCTCAAGCTCTACGTGCCACTTTTAGTGGCTTTACAAAGGAACAACAACGT
TTAGGGATCCCCATTGATCCCCGAGAATGGACAGACATGCATGTGAGAGAGTGGGTCTCG
TGGGCAGTGAATGAATTTACTCTGAAAGGAGTGGACTTTCAGAAGTTCTGTATGAGCGGA
GCAGCATTATGTGCCCTGGGGAAGGAATGCTTCCTAGAACTGGCACCAGACTTTGTGGGA
GATATCTTATGGGAGCACTTGGAGATCTTGCAGAAAGACTCAAAACAATACCAGACTAGT
GAAATAACCCCAGCATACCCAGAATCAAGATACACTTCAGACTACTTTATCAGTTATGGA
ATTGAACATGCACAGTGTGTTCCACCCTCTGAATTCTCTGAACCTGGCTTCATCACAGAG
TCATATCAGACCTTGCATCCCATCAGCTCAGAAGAACTCTTGTCTTTGAAATATGAGAAT
GACTACCCTCTAGGACTGCTGCGTGACCCCCTGCAGCCTGAATCTCTTCAGGGCGACTAC
TTCACCATCAAACAGGAGGTAGTGACTCCAGACAACATGTGCCTGGGACGCATCAGTCGT
GGAAAACTTGGTGGGCAGGAGTCCTTTGAAAGTATTGAGAGCCATGACAGCTGTGACCGC
CTCACACAGTCCTGGAGCAGCCAGTCCTCATACAACAGCCTTCAACGTGTGCCATCTTAT
GACAGTTTTGATTCGGAAGACTACCCACCTGCTATGCCGAGCCATAAATCCAAAGGCACT
TTTAAAGACTATGTAAGGGACAGAGCGGAACTCAACAAAGACAAACCTGTCATCCCTGCT
GCTGCTCTAGCTGGCTACACAGGAAGCGGACCAATTCAGCTGTGGCAGTTTCTCCTGGAG
TTACTGACAGACAAATCATGCCAGTCCTTTATCAGCTGGACAGGGGATGGCTGGGAATTT
AAGCTGTCTGATCCAGATGAGGTTGCAAGACGCTGGGGCAAGAGGAAAAACAAGCCTAAA
ATGAACTATGAGAAGTTGAGTCGTGGGCTCCGCTATTATTATGACAAAAATATAATTCAC
AAAACAGCAGGAAAGCGATACGTCTACCGTTTTGTATGCGACTTACAAAGTCTTCTGGGA
TATGTACCAGAAGAACTGCACGCCATGCTTGATGTTAAACCAGACACTGATGAATAG

Protein sequence predicted based on nucleotide sequence

MKAALDLKPTLSIKTEKEYEAYSCSDMECADVPLLTPSSKEMMSQALRATFSGFTKEQQR
LGIPIDPREWTDMHVREWVSWAVNEFTLKGVDFQKFCMSGAALCALGKECFLELAPDFVG
DILWEHLEILQKDSKQYQTSEITPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPGFITE
SYQTLHPISSEELLSLKYENDYPLGLLRDPLQPESLQGDYFTIKQEVVTPDNMCLGRISR
GKLGGQESFESIESHDSCDRLTQSWSSQSSYNSLQRVPSYDSFDSEDYPPAMPSHKSKGT
FKDYVRDRAELNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEF
KLSDPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQSLLG
YVPEELHAMLDVKPDTDE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable