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CYP4F22 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP4F22
Gene Name:  cytochrome P450, family 4, subfamily F, polypeptide 22
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00719199

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1593nts         Open reading frame : 1 to 1593

ATGCTGCCCATCACAGACCGCCTGCTGCACCTCCTGGGGCTGGAGAAGACGGCGTTCCGC
ATATACGCGGTGTCCACCCTTCTCCTCTTCCTGCTCTTCTTCCTGTTCCGCCTGCTGCTG
CGGTTCCTGAGGCTCTGCAGGAGCTTCTACATCACCTGCCGCCGGCTGCGCTGCTTCCCC
CAGCCTCCCCGGCGCAACTGGCTGCTGGGCCACCTGGGCATGTACCTTCCAAATGAGGCG
GGCCTTCAAGATGAGAAGAAGGTACTGGACAACATGCACCATGTACTCTTGGTATGGATG
GGACCTGTCCTGCCGCTGTTGGTTCTGGTGCACCCTGATTACATCAAACCCCTTTTGGGA
GCCTCAGCTGCCATCGCCCCCAAGGATGACCTCTTCTATGGCTTCCTAAAACCTTGGCTA
GGGGATGGGCTGCTGCTCAGCAAAGGTGACAAGTGGAGCCGGCACCGTCGCCTGCTGACA
CCCGCCTTCCACTTTGACATCCTGAAGCCTTACATGAAGATCTTCAACCAGAGCGCTGAC
ATTATGCATGCTAAATGGCGGCATCTGGCAGAGGGCTCAGCGGTCTCCCTTGATATGTTT
GAGCATATCAGCCTCATGACCCTGGACAGTCTTCAGAAATGTGTCTTCAGCTACAACAGC
AACTGCCAAGAGAAGATGAGTGATTATATCTCCGCTATCATTGAACTGAGCGCTCTGTCT
GTCCGGCGCCAGTATCGCTTGCACCACTACCTCGACTTCATTTACTACCGCTCGGCGGAT
GGGCGGAGGTTCCGGCAGGCCTGTGACATGGTGCACCACTTCACCACTGAAGTCATCCAG
GAACGGCGGCGGGCACTGCGTCAGCAGGGGGCCGAGGCCTGGCTTAAGGCCAAGCAGGGG
AAGACCTTGGACTTTATTGATGTGCTGCTCCTGGCCAGGGATGAAGATGGAAAGGAACTG
TCAGACGAGGATATCCGAGCCGAAGCAGACACCTTCATGTTTGAGGGTCACGACACAACA
TCCAGTGGGATCTCTTGGATGCTGTTCAATTTGGCAAAGTATCCGGAATACCAGGAGAAA
TGCCGAGAAGAGATTCAGGAAGTCATGAAAGGCCGGGAGCTGGAGGAGCTGGAGTGGGAC
GATCTGACTCAGCTGCCCTTTACAACTATGTGCATTAAGGAGAGCCTGCGCCAGTACCCA
CCTGTCACTCTTGTCTCTCGCCAATGCACGGAGGACATCAAGCTCCCAGATGGGCGCATC
ATCCCCAAAGGAATCATCTGCTTGGTCAGCATCTATGGAACCCACCACAACCCCACAGTG
TGGCCTGACTCCAAGGTGTACAACCCCTACCGCTTTGACCCGGACAACCCACAGCAGCGC
TCTCCACTGGCCTATGTGCCCTTCTCTGCAGGACCCAGGAATTGCATCGGACAGAGCTTC
GCCATGGCCGAGTTGCGCGTGGTTGTGGCACTAACACTGCTACGTTTCCGCCTGAGCGTG
GACCGAACGCGCAAGGTGCGGCGGAAGCCGGAGCTCATACTGCGCACGGAGAACGGGCTC
TGGCTCAAGGTGGAGCCGCTGCCTCCGCGGGCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Clone
  • Organism

Gene

Target GenBank: BC093894

 

Clone

Cloning Information : 703180
IMAGE ID : 7939739

Organism


Species Specific ID: 126410