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LGALS2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS2
Gene Name:  lectin, galactoside-binding, soluble, 2
Sequence             
Original Clone ID: FLH257780.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00073738

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 396nts         Open reading frame : 1 to 396
Reference Sequence Alignment

ATGACGGGGGAACTTGAGGTTAAGAACATGGACATGAAGCCGGGGTCAACCCTGAAGATC
ACAGGCAGCATCGCCGATGGCACTGATGGCTTTGTAATTAATCTGGGCCAGGGGACAGAC
AAGCTGAACCTGCATTTCAACCCTCGCTTCAGCGAATCCACCATTGTCTGCAACTCATTG
GACGGCAGCAACTGGGGGCAAGAACAACGGGAAGATCACCTGTGCTTCAGCCCAGGGTCA
GAGGTCAAGTTCACAGTGACCTTTGAGAGTGACAAATTCAAGGTGAAGCTGCCAGATGGG
CACGAGCTGACTTTTCCCAACAGGCTGGGTCACAGCCACCTGAGCTACCTGAGCGTAAGG
GGCGGGTTCAACATGTCCTCTTTCAAGTTAAAAGAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 548
Start on reference sequence 153
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGTTGGCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)CCAACTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : Mach1 T1-R
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS2
Symbol Nomenclature : LGALS2
SYNONYM : HL14
Designation : S-Lac lectin 2|beta-galactoside-binding lectin L-14-II|gal-2|galectin 2|galectin-2|lactose-binding lectin 2
Full Nomenclature : lectin, galactoside-binding, soluble, 2
GENEID : 3957
GI : 109451541
GenBank Accession : CU013399
HGNC : 6562
MIM : 150571
Vega : OTTHUMG00000150590
Target GenBank: CR456512

 

Reference Sequence Alignment

  

HsCD00073738      ATGACGGGGGAACTTGAGGTTAAGAACATGGACATGAAGCCGGGGTCAACCCTGAAGATC
NM_006498.2       ATGACGGGGGAACTTGAGGTTAAGAACATGGACATGAAGCCGGGGTCAACCCTGAAGATC
                  ************************************************************

HsCD00073738      ACAGGCAGCATCGCCGATGGCACTGATGGCTTTGTAATTAATCTGGGCCAGGGGACAGAC
NM_006498.2       ACAGGCAGCATCGCCGATGGCACTGATGGCTTTGTAATTAATCTGGGCCAGGGGACAGAC
                  ************************************************************

HsCD00073738      AAGCTGAACCTGCATTTCAACCCTCGCTTCAGCGAATCCACCATTGTCTGCAACTCATTG
NM_006498.2       AAGCTGAACCTGCATTTCAACCCTCGCTTCAGCGAATCCACCATTGTCTGCAACTCATTG
                  ************************************************************

HsCD00073738      GACGGCAGCAACTGGGGGCAAGAACAACGGGAAGATCACCTGTGCTTCAGCCCAGGGTCA
NM_006498.2       GACGGCAGCAACTGGGGGCAAGAACAACGGGAAGATCACCTGTGCTTCAGCCCAGGGTCA
                  ************************************************************

HsCD00073738      GAGGTCAAGTTCACAGTGACCTTTGAGAGTGACAAATTCAAGGTGAAGCTGCCAGATGGG
NM_006498.2       GAGGTCAAGTTCACAGTGACCTTTGAGAGTGACAAATTCAAGGTGAAGCTGCCAGATGGG
                  ************************************************************

HsCD00073738      CACGAGCTGACTTTTCCCAACAGGCTGGGTCACAGCCACCTGAGCTACCTGAGCGTAAGG
NM_006498.2       CACGAGCTGACTTTTCCCAACAGGCTGGGTCACAGCCACCTGAGCTACCTGAGCGTAAGG
                  ************************************************************

HsCD00073738      GGCGGGTTCAACATGTCCTCTTTCAAGTTAAAAGAA---
NM_006498.2       GGCGGGTTCAACATGTCCTCTTTCAAGTTAAAAGAATAA
                  ************************************   

Protein

SMART domain : Gal-bind_lectin : Galactoside-binding lectin
SMART domain : GLECT : Galectin
HPRD : 08859

Clone

Cloning Information : 257780
HIP Master Clone ID : 194396
IMAGE ID : 100000273
Original Clone ID : FLH257780.01L

Organism


TAX_ID : 9606
Species Specific ID: 3957

Genome

Chromosome : 22
Map Location : 22q13.1
Ensembl : ENSG00000100079

Reagents

Labome : LGALS2-antibody