| Gene: |
Gene Symbol:
PA3189 Gene Name: sugar ABC transporter permease Sequence Map: pDONR cofig.pdf |
|---|---|
| Original Clone ID: | FLH042769.01F |
| Keyword: | None |
| Species: | Pseudomonas aeruginosa |
| Type: | cDNA |
| Vector Name: | pDONR201 Format: FUSION |
| Source: | HIP |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / Yes |
| Publications: | None |
| Authors: |
HIP |
|
PaCD00007406 Price: Login for Pricing AVAILABLE No restriction Special MTA: None |
|
Insert sequence: 933nts
Open reading frame : 1 to 933 ATGGCGACCAATTCCCCTGTCTATCCCACGGCTCCGGTAGCGCCTCGTCGCGTCTCGCCA
CTCGATCGCCTGCAAGGCTGGCTGCCGAAGCTGGTGCTGGCGCCAAGCGTGGTCGTGGTG
CTGTTCTGCATGTATGGCTACATTGGCTGGACGCTACTGCTGTCCTTCACCAACTCGCGC
TTCATGCCCAGCTACAAGTGGGCCGGGCTGAGCCAGTACCAGCGCCTGTGGGACAACGAC
CGCTGGTGGGTGGCGAGCAAGAACCTGCTGCTCTTCGGCGGCTCGTTCATCGCCATCTGC
CTGGTGCTCGGAGTGTTCCTCGCAGTGCTGCTGGACCAGCGCATCCGCCGCGAAGGCTTC
ATCCGCACCCTCTACCTGTATCCCATGGCGTTGTCGATGATCGTCACCGGCACCGCCTGG
AAGTGGCTGCTCAATCCGGGCCTGGGCCTGGACAAGCTGCTCCGCGACTGGGGCTGGGAA
GGTTTCCGCTTCGACTGGCTGGTGGACCCGGAGCGGGTCGTCTACTGCCTGGTGATCGCC
GCCGTGTGGCAGGCCTCCGGCTTCGTCATGGCGCTGTTCCTCGCCGGCCTGCGCGGGGTC
GACCCGGCCATCGTGCGCGCCGCCCAGGTCGACGGTGCGAGCCTGCCGACCATCTACCTG
CGCATCGTCCTGCCGAGCCTGCGCCCGGTGTTCTTCAGCGCGCTGATGATCCTCGCCCAT
ATCGCGATCAAGAGCTTCGACCTGGTGGCGGCGATGACCGCCGGCGGCCCGGGTTATTCC
TCCGACCTGCCGGCGATGTTCATGTATGCGCATACCTTCACCCGTGGCCAGATGGGGCTC
GGCGCGGCGAGCGCGATGCTGATGCTCGGCGCTGTACTGGCGATCGTGGTGCCTTACCTG
TATTCCGAACTGCGAGGCAAGCGCCATGCGGGA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Reference Sequence Annotations: Start on reference sequence 1 End on reference sequence 933 |
|
|
Mutations: None |
Discrepancy:g156a
|
5' Linker Sequence:
5p ACAAGTTTGTACAAAAAAGCAGGCTCCGAAGGAGATACC(atg-goi) |
|
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTACAAAGTGGT |
|
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
| Protein Expression Results: | None |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pDONR201 |
pDONR201 in advanced viewed |
| Synonyms: | '' | |
| Sequencing Primer: | Forward:
pDONR201-forward
Reverse: pDONR201-reverse |
|
| Description: | Recombinational donor/master vector; kanamycin resistance; recombinational cloning. | |
| Comments: | The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website. | |
| Size (bp): | 4470 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | Gateway, donor (entry), recombinational cloning | |
| Author Name: |
Invitrogen |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ColE | ColE1 (pUC-type) origin of replication (modified, low copy number) | 3744 | 4426 |
| negative selection marker | ccdB | ccdB negative selection gene (death cassette), LOST in recombined (with insert) form | 1264 | 959 |
| primer site | forward primer | recommended forward primer site 5’ tcgcgttaacgctagcatggatctc 3’ | 300 | 324 |
| primer site | reverse primer | recommended reverse primer site 5’ gtaacatcagagattttgagacac 3’ | 2769 | 2792 |
| recombination site | attP1 | attP recombination site 1 LOST in recombined (with insert) form | 332 | 563 |
| recombination site | attP2 | attP recombination site 2 LOST in recombined (with insert) form | 2744 | 2513 |
| recombination site | attL1 | attL recombination site 1 present in recombined (with insert) form only, position is approximate | 332 | 563 |
| recombination site | attL2 | attL recombination site 2 present in recombined (with insert) form only, position is approximate | 2744 | 2513 |
| selectable marker | CmR | chloramphenicol resistance gene, LOST in recombined (with insert) form | 2265 | 2513 |
| selectable marker | KanR | kanamycin resistance gene | 2868 | 3677 |
| trxn termination sequence | rrn T2 | rrn T2 transcription termination sequence | 73 | 100 |
| trxn termination sequence | rrn T1 | rrn T1 transcription termination sequence | 232 | 275 |
- Gene
- Protein
- Clone
- Organism
- Reagents
Gene
| Gene Symbol : | PA3189 |
|---|---|
| GENEID : | 882900 |
| Locus Tag : | PA3189 |
| GI : | 49085021 |
| GenBank Accession : | AY658971 |
| Pseudomonas aeruginosa (PA) number : | PA3189 |
| Target GenBank: | NP_251879 |
Protein
| UniProt : | Q9HZ49 |
|---|
Clone
| Cloning Information : | 42769 |
|---|---|
| HIP Master Clone ID : | 25716 |
| Original Clone ID : | FLH042769.01F |
Organism
| TAX_ID : | 208964 |
|---|---|
| Species Specific ID: | 882900 |
Reagents
| Labome : | PA3189-cDNA |
|---|
