Gene:
Gene Symbol:
PA3189
Gene Name:
sugar ABC transporter permease
Original Clone ID:
FLH042769.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
PaCD00007406
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 933nts
Open reading frame: 1 to 933
ATGGCGACCAATTCCCCTGTCTATCCCACGGCTCCGGTAGCGCCTCGTCGCGTCTCGCCA
CTCGATCGCCTGCAAGGCTGGCTGCCGAAGCTGGTGCTGGCGCCAAGCGTGGTCGTGGTG
CTGTTCTGCATGTATGGCTACATTGGCTGGACGCTACTGCTGTCCTTCACCAACTCGCGC
TTCATGCCCAGCTACAAGTGGGCCGGGCTGAGCCAGTACCAGCGCCTGTGGGACAACGAC
CGCTGGTGGGTGGCGAGCAAGAACCTGCTGCTCTTCGGCGGCTCGTTCATCGCCATCTGC
CTGGTGCTCGGAGTGTTCCTCGCAGTGCTGCTGGACCAGCGCATCCGCCGCGAAGGCTTC
ATCCGCACCCTCTACCTGTATCCCATGGCGTTGTCGATGATCGTCACCGGCACCGCCTGG
AAGTGGCTGCTCAATCCGGGCCTGGGCCTGGACAAGCTGCTCCGCGACTGGGGCTGGGAA
GGTTTCCGCTTCGACTGGCTGGTGGACCCGGAGCGGGTCGTCTACTGCCTGGTGATCGCC
GCCGTGTGGCAGGCCTCCGGCTTCGTCATGGCGCTGTTCCTCGCCGGCCTGCGCGGGGTC
GACCCGGCCATCGTGCGCGCCGCCCAGGTCGACGGTGCGAGCCTGCCGACCATCTACCTG
CGCATCGTCCTGCCGAGCCTGCGCCCGGTGTTCTTCAGCGCGCTGATGATCCTCGCCCAT
ATCGCGATCAAGAGCTTCGACCTGGTGGCGGCGATGACCGCCGGCGGCCCGGGTTATTCC
TCCGACCTGCCGGCGATGTTCATGTATGCGCATACCTTCACCCGTGGCCAGATGGGGCTC
GGCGCGGCGAGCGCGATGCTGATGCTCGGCGCTGTACTGGCGATCGTGGTGCCTTACCTG
TATTCCGAACTGCGAGGCAAGCGCCATGCGGGA
Protein sequence predicted based on nucleotide sequence
MATNSPVYPTAPVAPRRVSPLDRLQGWLPKLVLAPSVVVVLFCMYGYIGWTLLLSFTNSR FMPSYKWAGLSQYQRLWDNDRWWVASKNLLLFGGSFIAICLVLGVFLAVLLDQRIRREGF IRTLYLYPMALSMIVTGTAWKWLLNPGLGLDKLLRDWGWEGFRFDWLVDPERVVYCLVIA AVWQASGFVMALFLAGLRGVDPAIVRAAQVDGASLPTIYLRIVLPSLRPVFFSALMILAH IAIKSFDLVAAMTAGGPGYSSDLPAMFMYAHTFTRGQMGLGAASAMLMLGAVLAIVVPYL YSELRGKRHAG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
933
Mutations:
None
Discrepancy:
g156a
5' Linker Sequence:
5p ACAAGTTTGTACAAAAAAGCAGGCTCCGAAGGAGATACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTACAAAGTGGT
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 (pUC-type) origin of replication (modified, low copy number)
3744
4426
negative selection marker
ccdB
ccdB negative selection gene (death cassette), LOST in recombined (with insert) form
1264
959
primer site
forward primer
recommended forward primer site 5’ tcgcgttaacgctagcatggatctc 3’
300
324
primer site
reverse primer
recommended reverse primer site 5’ gtaacatcagagattttgagacac 3’
2769
2792
recombination site
attP1
attP recombination site 1 LOST in recombined (with insert) form
332
563
recombination site
attP2
attP recombination site 2 LOST in recombined (with insert) form
2744
2513
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
selectable marker
CmR
chloramphenicol resistance gene, LOST in recombined (with insert) form
2265
2513
selectable marker
KanR
kanamycin resistance gene
2868
3677
trxn termination sequence
rrn T2
rrn T2 transcription termination sequence
73
100
trxn termination sequence
rrn T1
rrn T1 transcription termination sequence
232
275
