MAD1L1 (Homo sapiens) in pJFT7_nHalo_DC(r4)

Explanation of Terms

Gene:
Gene Symbol:  MAD1L1
Gene Name:  MAD1 mitotic arrest deficient-like 1 (yeast)
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format: 
CLOSED (STOP codon in vector)
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00741334
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2154nts
Open reading frame: 1 to 2154

GAAGACCTGGGGGAAAACACCATGGTTTTATCCACCCTGAGATCTTTGAACAACTTCATC
TCTCAGCGTGTGGAGGGAGGCTCTGGACTGGATATTTCTACCTCGGCCCCAGGTTCTCTG
CAGATGCAGTACCAGCAGAGCATGCAGCTGGAGGAAAGAGCAGAGCAGATCCGTTCGAAG
TCCCACCTCATCCAGGTGGAGCGGGAGAAAATGCAGATGGAGCTGAGTCACAAGAGGGCT
CGAGTGGAGCTGGAGAGAGCAGCCAGCACCAGTGCCAGGAACTACGAGCGTGAGGTCGAC
CGCAACCAGGAGCTCCTGACGCGCATCCGGCAGCTTCAGGAGCGGGAGGCCGGGGCGGAG
GAGAAGATGCAGGAGCAGCTGGAGCGCAACAGGCAGTGTCAGCAGAACTTGGATGCTGCC
AGCAAGAGGCTGCGTGAGAAAGAGGACAGTCTGGCCCAGGCTGGCGAGACCATCAACGCA
CTGAAGGGGAGGATCTCGGAACTGCAGTGGAGCGTGATGGACCAGGAGATGCGGGTGAAG
CGCCTGGAGTCGGAGAAGCAGGAGCTGCAGGAGCAGCTGGACCTGCAACACAAAAAATGC
CAGGAAGCCAATCAGAAAATCCAGGAACTCCAGGCCAGCCAAGAAGCAAGAGCAGACCAC
GAGCAGCAGATTAAGGATCTGGAGCAGAAGCTGTCCCTGCAAGAGCAGGATGCAGCGATT
GTGAAGAACATGAAGTCTGAGCTGGTACGGCTCCCTAGGCTGGAACGGGAGCTGAAGCAG
CTGCGGGAGGAGAGCGCGCACCTGCGGGAGATGAGAGAGACCAACGGGCTGCTCCAGGAA
GAGCTGGAAGGGCTGCAGAGGAAGCTGGGGCGCCAGGAGAAGATGCAGGAGACGCTGGTT
GGCTTGGAGCTGGAGAACGAGAGGCTGCTGGCCAAGCTGCAAAGCTGGGAGAGACTGGAC
CAGACCATGGGCCTGAGCATCAGGACTCCAGAAGACCTTTCCAGATTCGTGGTTGAGCTG
CAGCAGAGGGAGCTTGCCTTGAAGGACAAGAACAGCGCCGTCACCAGCAGCGCCCGGGGG
CTGGAGAAGGCCAGGCAGCAGCTGCAGGAGGAGCTCCGGCAGGTCAGCGGCCAGCTGTTG
GAGGAGAGGAAGAAGCGCGAGACCCACGAGGCGCTGGCCCGGAGGCTCCAGAAACGGGTC
CTGCTGCTCACCAAGGAGCGGGACGGTATGCGGGCCATCCTGGGGTCCTACGACAGCGAG
CTGACCCCGGCCGAGTACTCACCCCAGCTGACGCGGCGCATGCGGGAGGCTGAGGATATG
GTGCAGAAGGTGCACAGCCACAGCGCCGAGATGGAGGCTCAGCTGTCGCAGGCCCTGGAG
GAGCTGGGAGGCCAGAAACAAAGAGCAGACATGCTGGAGATGGAGCTGAAGATGCTGAAG
TCTCAGTCCAGCTCTGCCGAACAGAGCTTCCTGTTCTCCAGGGAGGAGGCGGACACGCTC
AGGTTGAAGGTCGAGGAGCTGGAAGGCGAGCGGAGTCGGCTGGAGGAGGAAAAGAGGATG
CTGGAGGCACAGCTGGAGCGGCGAGCTCTGCAGGGTGACTATGACCAGAGCAGGACCAAA
GTGCTGCACATGAGCCTGAACCCCACCAGTGTGGCCAGGCAGCGCCTGCGCGAGGACCAC
AGCCAGCTGCAGGCGGAGTGCGAGCGACTGCGCGGGCTCCTGCGCGCCATGGAGAGAGGA
GGCACCGTCCCAGCCGACCTTGAGGCTGCCGCCGCGAGTCTGCCATCGTCCAAGGAGGTG
GCAGAGCTGAAGAAGCAGGTGGAGAGTGCCGAGCTGAAGAACCAGCGGCTCAAGGAGGTT
TTCCAGACCAAGATCCAGGAGTTCCGCAAGGCCTGCTACACGCTCACCGGCTACCAGATC
GACATCACCACGGAGAACCAGTACCGGCTGACCTCGCTGTACGCCGAGCACCCAGGCGAC
TGCCTCATCTTCAAGGCCACCAGCCCCTCGGGTTCCAAGATGCAGCTACTGGAGACAGAG
TTCTCACACACCGTGGGCGAGCTCATCGAGGTGCACCTGCGGCGCCAGGACAGCATCCCT
GCCTTCCTCAGCTCGCTCACCCTCGAGCTCTTCAGCCGCCAGACCGTGGCGTGG

Protein sequence predicted based on nucleotide sequence

EDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQLEERAEQIRSK
SHLIQVEREKMQMELSHKRARVELERAASTSARNYEREVDRNQELLTRIRQLQEREAGAE
EKMQEQLERNRQCQQNLDAASKRLREKEDSLAQAGETINALKGRISELQWSVMDQEMRVK
RLESEKQELQEQLDLQHKKCQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAAI
VKNMKSELVRLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETLV
GLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARG
LEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKRVLLLTKERDGMRAILGSYDSE
LTPAEYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLK
SQSSSAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTK
VLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEV
AELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGD
CLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSIPAFLSSLTLELFSRQTVAW

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable