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ARAF1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARAF
Gene Name:  v-raf murine sarcoma 3611 viral oncogene homolog
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008256.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000744

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1821nts         Open reading frame : 1 to 1821
Reference Sequence Alignment

ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGAT
TTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTA
ACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT
GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGTCCATATG
GTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAGTTTCAGC
ACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCCCAGCCCA
GCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC
TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCG
TTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTG
TCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCTCAGGAAG
ACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATC
ATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC
TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGG
CTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCC
CAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATG
CAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC
GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGATTATCTTT
ATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTGCCCCAAG
GCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGT
GCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC
GCAGCCCGCCTTGTGCCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2023
Start on reference sequence 203
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARAF
Symbol Nomenclature : ARAF
SYNONYM : A-RAF; ARAF1; PKS2; RAFA1
Designation : A-Raf proto-oncogene serine/threonine-protein kinase|Oncogene ARAF1|Ras-binding protein DA-Raf|proto-oncogene A-Raf-1|proto-oncogene Pks|serine/threonine-protein kinase A-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
Full Nomenclature : v-raf murine sarcoma 3611 viral oncogene homolog
GENEID : 369
Locus Tag : RP1-230G1.1
GI : 61357088
GenBank Accession : AY888388
HGNC : 646
MIM : 311010
Vega : OTTHUMG00000021446
Target GenBank: NM_001654

 

Reference Sequence Alignment

  

HsCD00000744        ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
NM_001654.4         ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
NM_001256196.1      ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
                    ************************************************************

HsCD00000744        GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
NM_001654.4         GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
NM_001256196.1      GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
                    ************************************************************

HsCD00000744        GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
NM_001654.4         GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
NM_001256196.1      GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
                    ************************************************************

HsCD00000744        GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
NM_001654.4         GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
NM_001256196.1      GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
                    ************************************************************

HsCD00000744        CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
NM_001654.4         CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
NM_001256196.1      CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
                    ************************************************************

HsCD00000744        TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
NM_001654.4         TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
NM_001256196.1      TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
                    ************************************************************

HsCD00000744        CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
NM_001654.4         CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
NM_001256196.1      CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
                    ************************************************************

HsCD00000744        CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAG---------TTCTACCACAGT
NM_001654.4         CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAG---------TTCTACCACAGT
NM_001256196.1      CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGCCCTCAAGGTTCTACCACAGT
                    ***************************************         ************

HsCD00000744        GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
NM_001654.4         GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
NM_001256196.1      GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
                    ************************************************************

HsCD00000744        GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
NM_001654.4         GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
NM_001256196.1      GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
                    ************************************************************

HsCD00000744        CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
NM_001654.4         CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
NM_001256196.1      CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
                    ************************************************************

HsCD00000744        GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
NM_001654.4         GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
NM_001256196.1      GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
                    ************************************************************

HsCD00000744        AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
NM_001654.4         AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
NM_001256196.1      AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
                    ************************************************************

HsCD00000744        CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
NM_001654.4         CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
NM_001256196.1      CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
                    ************************************************************

HsCD00000744        CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
NM_001654.4         CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
NM_001256196.1      CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
                    ************************************************************

HsCD00000744        GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
NM_001654.4         GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
NM_001256196.1      GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
                    ************************************************************

HsCD00000744        ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
NM_001654.4         ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
NM_001256196.1      ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
                    ************************************************************

HsCD00000744        CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
NM_001654.4         CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
NM_001256196.1      CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
                    ************************************************************

HsCD00000744        CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
NM_001654.4         CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
NM_001256196.1      CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
                    ************************************************************

HsCD00000744        TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
NM_001654.4         TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
NM_001256196.1      TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
                    ************************************************************

HsCD00000744        GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
NM_001654.4         GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
NM_001256196.1      GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
                    ************************************************************

HsCD00000744        GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
NM_001654.4         GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
NM_001256196.1      GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
                    ************************************************************

HsCD00000744        CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
NM_001654.4         CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
NM_001256196.1      CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
                    ************************************************************

HsCD00000744        AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
NM_001654.4         AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
NM_001256196.1      AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
                    ************************************************************

HsCD00000744        ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
NM_001654.4         ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
NM_001256196.1      ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
                    ************************************************************

HsCD00000744        GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
NM_001654.4         GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
NM_001256196.1      GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
                    ************************************************************

HsCD00000744        ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
NM_001654.4         ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
NM_001256196.1      ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
                    ************************************************************

HsCD00000744        TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
NM_001654.4         TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
NM_001256196.1      TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
                    ************************************************************

HsCD00000744        CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
NM_001654.4         CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
NM_001256196.1      CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
                    ************************************************************

HsCD00000744        GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
NM_001654.4         GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
NM_001256196.1      GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
                    ************************************************************

HsCD00000744        CTACTCAGCGCAGCCCGCCTTGTGCCTTAG
NM_001654.4         CTACTCAGCGCAGCCCGCCTTGTGCCTTAG
NM_001256196.1      CTACTCAGCGCAGCCCGCCTTGTGCCTTAG
                    ******************************

Pathways

Panther : Angiogenesis
Panther : B cell activation
Panther : Integrin signalling pathway
Panther : Interleukin signaling pathway
Panther : PDGF signaling pathway
Panther : Ras Pathway
Panther : T cell activation
Panther : VEGF signaling pathway
WikiPathway : ErbB signaling pathway
WikiPathway : Focal Adhesion
WikiPathway : Integrated Breast Cancer Pathway
WikiPathway : Integrin-mediated cell adhesion
WikiPathway : MAPK Cascade

Clone

Cloning Information : 8256
HIP Master Clone ID : 7814
Original Clone ID : FLH008256.01X

Organism


TAX_ID : 9606
Species Specific ID: 369

Genome

Chromosome : X
Map Location : Xp11.4-p11.2
Ensembl : ENSG00000078061

Reagents

Labome : ARAF-antibody