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UBL1 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SUMO1
Gene Name:  small ubiquitin-like modifier 1
Sequence             
Original Clone ID: FLH213315.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00074669

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 306nts         Open reading frame : 1 to 306
Reference Sequence Alignment

ATGTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAA
TATATTAAACTCAAAGTCATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATG
ACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAAT
TCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTG
GGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGAGGTCATTCAACA
GTTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 437
Start on reference sequence 132
Mutations:
None
Discrepancy:
g288a
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SUMO1
Symbol Nomenclature : SUMO1
SYNONYM : DAP1; GMP1; OFC10; PIC1; SENP2; SMT3; SMT3C; SMT3H3; UBL1
Designation : GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|small ubiquitin-related modifier 1|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
Full Nomenclature : small ubiquitin-like modifier 1
GENEID : 7341
Locus Tag : OK/SW-cl.43
HGNC : 12502
MIM : 601912
Vega : OTTHUMG00000132839
Target GenBank: BC006462

 

Reference Sequence Alignment

  

HsCD00074669        ATGTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAA
NM_001005781.1      ATGTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAA
NM_003352.4         ATGTCTGACCAGGAGGCAAAACCTTCAACTGAGGACTTGGGGGATAAGAAGGAAGGTGAA
                    ************************************************************

HsCD00074669        TATATTAAACTCAAAGTCATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATG
NM_001005781.1      TATATTAAACTCAAAGTCATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATG
NM_003352.4         TATATTAAACTCAAAGTCATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATG
                    ************************************************************

HsCD00074669        ACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAAT
NM_001005781.1      ACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAAT
NM_003352.4         ACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAAT
                    ************************************************************

HsCD00074669        TCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTG
NM_001005781.1      TCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTG
NM_003352.4         TCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTCCAAAAGAACTG
                    ************************************************************

HsCD00074669        GGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGAGGTCATTCAACA
NM_001005781.1      GGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGGGGTCATTCAACA
NM_003352.4         GGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGGGGTCATTCAACA
                    ***********************************************.************

HsCD00074669        GTTTAG
NM_001005781.1      GTTTAG
NM_003352.4         GTTTAG
                    ******

Pathways

NCI : Signaling events mediated by HDAC Class I
NCI : Signaling events mediated by HDAC Class II
NCI : Sumoylation by RanBP2 regulates transcriptional repression
Panther : p53 pathway
Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Interferon Signaling
Reactome : Interferon gamma signaling
Reactome : Metabolism of proteins
Reactome : Post-translational protein modification
Reactome : Processing and activation of SUMO
Reactome : Regulation of IFNG signaling
Reactome : SUMO is conjugated to E1 (UBA2:SAE1)
Reactome : SUMO is proteolytically processed
Reactome : SUMO is transferred from E1 to E2 (UBE2I, UBC9)
Reactome : SUMOylation
WikiPathway : Androgen receptor signaling pathway
WikiPathway : Diurnally regulated genes with circadian orthologs
WikiPathway : TGF beta Signaling Pathway
WikiPathway : TP53 network

Protein

SMART domain : UBQ : Ubiquitin homologues
UniProt : A8MUS8
UniProt : P63165
HPRD : 03554

Clone

Cloning Information : 213315
HIP Master Clone ID : 26433
Original Clone ID : FLH213315.01X

Organism


TAX_ID : 9606
Species Specific ID: 7341

Genome

Chromosome : 2
Map Location : 2q33
Ensembl : ENSG00000116030

Reagents

Labome : SUMO-1-antibody