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CAPN11 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN11
Gene Name:  calpain 11
Sequence             
Original Clone ID: FLH217604.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00074809

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2109nts         Open reading frame : 1 to 2109
Reference Sequence Alignment

ATGGTGGCTCACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAAC
GCCCAGAACTTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGG
GAGCTCTTCGAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTG
GGCCCCAACTCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAAC
AACCCTCTATTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGG
GACTGCTGGCTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGC
GTGGTGCCCAGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATT
TGGCAGTTTGGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGAC
AAGCTGGTGTTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAG
GCGTATGCCAAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGC
CTTGAGGACTTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAAC
CTGCTCAGGCTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAA
GTCACCAGTGATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCT
TACTCTGTGACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGG
GTCCGGAATCCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAG
TGGGAAGAGGTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAG
TTCTGGATGTCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTC
ACGCCTGATACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGC
AGCTGGCGCAGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACC
AACCCCCAGTTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGC
AATGTTGTGGCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGG
CAGCAGGGAGCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTT
CAGAACATTCAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGC
TTCTCAGAGATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGG
GAATATATCATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGG
GTCTTCACCGAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAA
CTCCAAGAGGAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAG
ATAGTGGCAGGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGG
ATGGCCATCAAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGC
ATGATCAACCTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATC
CTGTGGAAAAAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCA
GGCACCTTGAACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAAC
AACAAGGTAATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTT
GACAGCTTCATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATG
GACCCCAAGAATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATG
TGGGGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 2229
Start on reference sequence 121
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN11
Symbol Nomenclature : CAPN11
SYNONYM : calpain11
Designation : CANP 11|calcium-activated neutral proteinase 11|calpain-11
Full Nomenclature : calpain 11
GENEID : 11131
HGNC : 1478
MIM : 604822
Vega : OTTHUMG00000014758
Target GenBank: BC033733

 

Reference Sequence Alignment

  

HsCD00074809      ------------------------------------------------------------
NM_007058.3       ATGCTGTACTCCCCAGGGCCGAGTCTTCCGGAGTCAGCAGAGAGCCTGGATGGATCACAG
                                                                              

HsCD00074809      ---------------------------------------------------ATGGTGGCT
NM_007058.3       GAGGATAAGCCTCGGGGCTCATGTGCGGAGCCCACTTTTACTGATACGGGAATGGTGGCT
                                                                     *********

HsCD00074809      CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
NM_007058.3       CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
                  ************************************************************

HsCD00074809      TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
NM_007058.3       TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
                  ************************************************************

HsCD00074809      GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
NM_007058.3       GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
                  ************************************************************

HsCD00074809      TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
NM_007058.3       TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
                  ************************************************************

HsCD00074809      TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
NM_007058.3       TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
                  ************************************************************

HsCD00074809      CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
NM_007058.3       CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
                  ************************************************************

HsCD00074809      AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
NM_007058.3       AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
                  ************************************************************

HsCD00074809      GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
NM_007058.3       GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
                  ************************************************************

HsCD00074809      TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
NM_007058.3       TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
                  ************************************************************

HsCD00074809      AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
NM_007058.3       AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
                  ************************************************************

HsCD00074809      TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
NM_007058.3       TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
                  ************************************************************

HsCD00074809      CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
NM_007058.3       CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
                  ************************************************************

HsCD00074809      GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
NM_007058.3       GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
                  ************************************************************

HsCD00074809      ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
NM_007058.3       ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
                  ************************************************************

HsCD00074809      CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
NM_007058.3       CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
                  ************************************************************

HsCD00074809      GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
NM_007058.3       GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
                  ************************************************************

HsCD00074809      TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
NM_007058.3       TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
                  ************************************************************

HsCD00074809      ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
NM_007058.3       ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
                  ************************************************************

HsCD00074809      AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
NM_007058.3       AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
                  ************************************************************

HsCD00074809      TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
NM_007058.3       TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
                  ************************************************************

HsCD00074809      GCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
NM_007058.3       GTCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
                  * **********************************************************

HsCD00074809      GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
NM_007058.3       GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
                  ************************************************************

HsCD00074809      CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
NM_007058.3       CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
                  ************************************************************

HsCD00074809      ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
NM_007058.3       ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
                  ************************************************************

HsCD00074809      ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
NM_007058.3       ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
                  ************************************************************

HsCD00074809      GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
NM_007058.3       GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
                  ************************************************************

HsCD00074809      GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
NM_007058.3       GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
                  ************************************************************

HsCD00074809      GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
NM_007058.3       GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
                  ************************************************************

HsCD00074809      AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
NM_007058.3       AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
                  ************************************************************

HsCD00074809      CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
NM_007058.3       CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
                  ************************************************************

HsCD00074809      AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
NM_007058.3       AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
                  ************************************************************

HsCD00074809      AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
NM_007058.3       AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
                  ************************************************************

HsCD00074809      ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
NM_007058.3       ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
                  ************************************************************

HsCD00074809      ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
NM_007058.3       ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
                  ************************************************************

HsCD00074809      AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
NM_007058.3       AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
                  ************************************************************

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q9UMQ6
HPRD : 05315

Clone

Cloning Information : 217604
HIP Master Clone ID : 95513
Original Clone ID : FLH217604.01X

Organism


TAX_ID : 9606
Species Specific ID: 11131

Genome

Chromosome : 6
Map Location : 6p12
Ensembl : ENSG00000137225

Reagents

Labome : CAPN11-antibody