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TSG101 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TSG101
Gene Name:  tumor susceptibility 101
Sequence             
Original Clone ID: FLH251610.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00075230

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1173nts         Open reading frame : 1 to 1173
Reference Sequence Alignment

ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
ACTATCTTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1257
Start on reference sequence 85
Mutations:
None
Discrepancy:
c1027t,L343F
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TSG101
Symbol Nomenclature : TSG101
SYNONYM : TSG10; VPS23
Designation : ESCRT-I complex subunit TSG101|tumor susceptibility gene 10|tumor susceptibility gene 101|tumor susceptibility gene 101 protein|tumor susceptibility protein
Full Nomenclature : tumor susceptibility 101
GENEID : 7251
HGNC : 15971
MIM : 601387
Vega : OTTHUMG00000167725
Target GenBank: BC002487

 

Reference Sequence Alignment

  

HsCD00075230      ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
NM_006292.3       ATGGCGGTGTCGGAGAGCCAGCTCAAGAAAATGGTGTCCAAGTACAAATACAGAGACCTA
                  ************************************************************

HsCD00075230      ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
NM_006292.3       ACTGTACGTGAAACTGTCAATGTTATTACTCTATACAAAGATCTCAAACCTGTTTTGGAT
                  ************************************************************

HsCD00075230      TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
NM_006292.3       TCATATGTTTTTAACGATGGCAGTTCCAGGGAACTAATGAACCTCACTGGAACAATCCCT
                  ************************************************************

HsCD00075230      GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
NM_006292.3       GTGCCTTATAGAGGTAATACATACAATATTCCAATATGCCTATGGCTACTGGACACATAC
                  ************************************************************

HsCD00075230      CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
NM_006292.3       CCATATAATCCCCCTATCTGTTTTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGA
                  ************************************************************

HsCD00075230      AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
NM_006292.3       AAGCATGTTGATGCAAATGGGAAGATATATCTTCCTTATCTACATGAATGGAAACACCCA
                  ************************************************************

HsCD00075230      CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
NM_006292.3       CAGTCAGACTTGTTGGGGCTTATTCAGGTCATGATTGTGGTATTTGGAGATGAACCTCCA
                  ************************************************************

HsCD00075230      GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
NM_006292.3       GTCTTCTCTCGTCCTATTTCGGCATCCTATCCGCCATACCAGGCAACGGGGCCACCAAAT
                  ************************************************************

HsCD00075230      ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
NM_006292.3       ACTTCCTACATGCCAGGCATGCCAGGTGGAATCTCTCCATACCCATCCGGATACCCTCCC
                  ************************************************************

HsCD00075230      AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
NM_006292.3       AATCCCAGTGGTTACCCAGGCTGTCCTTACCCACCTGGTGGTCCATATCCTGCCACAACA
                  ************************************************************

HsCD00075230      AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
NM_006292.3       AGTTCTCAGTACCCTTCTCAGCCTCCTGTGACCACTGTTGGTCCCAGTAGGGATGGCACA
                  ************************************************************

HsCD00075230      ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
NM_006292.3       ATCAGCGAGGACACCATCCGAGCCTCTCTCATCTCTGCGGTCAGTGACAAACTGAGATGG
                  ************************************************************

HsCD00075230      CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
NM_006292.3       CGGATGAAGGAGGAAATGGATCGTGCCCAGGCAGAGCTCAATGCCTTGAAACGAACAGAA
                  ************************************************************

HsCD00075230      GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
NM_006292.3       GAAGACCTGAAAAAGGGTCACCAGAAACTGGAAGAGATGGTTACCCGTTTAGATCAAGAA
                  ************************************************************

HsCD00075230      GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
NM_006292.3       GTAGCCGAGGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTCAGTTCT
                  ************************************************************

HsCD00075230      GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
NM_006292.3       GCTCTGGAAAAAATGGAAAATCAGTCTGAAAACAATGATATCGATGAAGTTATCATTCCC
                  ************************************************************

HsCD00075230      ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
NM_006292.3       ACAGCTCCCTTATACAAACAGATCCTGAATCTGTATGCAGAAGAAAACGCTATTGAAGAC
                  ************************************************************

HsCD00075230      ACTATCTTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
NM_006292.3       ACTATCTTTTACTTGGGAGAAGCCTTGAGAAGGGGCGTGATAGACCTGGATGTCTTCCTG
                  ************************************************************

HsCD00075230      AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
NM_006292.3       AAGCATGTACGTCTTCTGTCCCGTAAACAGTTCCAGCTGAGGGCACTAATGCAAAAAGCA
                  ************************************************************

HsCD00075230      AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA
NM_006292.3       AGAAAGACTGCCGGTCTCAGTGACCTCTACTGA
                  *********************************

Pathways

NCI : Glucocorticoid receptor regulatory network
NCI : Internalization of ErbB1
Reactome : Assembly Of The HIV Virion
Reactome : Budding and maturation of HIV virion
Reactome : Disease
Reactome : Endosomal Sorting Complex Required For Transport (ESCRT)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : Late Phase of HIV Life Cycle
Reactome : Membrane Trafficking
Reactome : Membrane binding and targetting of GAG proteins

Clone

Cloning Information : 251610
HIP Master Clone ID : 33331
Original Clone ID : FLH251610.01X

Organism


TAX_ID : 9606
Species Specific ID: 7251

Genome

Chromosome : 11
Map Location : 11p15
Ensembl : ENSG00000074319

Reagents

Labome : TSG101-antibody