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THOC3 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  THOC3
Gene Name:  THO complex 3
Sequence             
Original Clone ID: FLH251718.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00075304

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1056nts         Open reading frame : 1 to 1056
Reference Sequence Alignment

ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCG
ATGGCCCCGTGGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAG
CTGTTCCGGGGCCACAGCAAGACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCG
GTGGCCTGGAGTTGCGACGGGCGTCGCCTAGCCTCGGGGTCCTTCGACAAGACGGCCAGC
GTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACAATTATCGGGGACATGGGGAT
AGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTACGGCGTCTGGA
GATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAAC
AAGGATGATGTGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAG
TTCAAGTTCGAGGTCAACGAAATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACA
AATGGCAATGGTTGTATCAACATCCTCAGCTACCCAGAACTGAAGCCTGTGCAGTCCATC
AACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACCCCATGGGGAAGTACTTTGCC
ACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGTGTGTGTTCGG
TGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAA
CTATGGGAGGTACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCT
CTGCTGGCATTTGCCTGTGATGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGA
ACTGTGAAGCTGTTTGGGCTTCCTAATGATTCTTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1125
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : THOC3
Symbol Nomenclature : THOC3
SYNONYM : THO3; hTREX45
Designation : TEX1 homolog|THO complex subunit 3
Full Nomenclature : THO complex 3
GENEID : 84321
HGNC : 19072
MIM : 606929
Vega : OTTHUMG00000130658
Target GenBank: BC006849

 

Reference Sequence Alignment

  

HsCD00075304      ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCG
NM_032361.3       ATGGCGGTCCCCGCTGCAGCCATGGGGCCCTCGGCGTTGGGCCAGAGCGGCCCCGGCTCG
                  ************************************************************

HsCD00075304      ATGGCCCCGTGGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAG
NM_032361.3       ATGGCCCCGTGGTGCTCAGTGAGCAGCGGCCCGTCGCGCTACGTGCTTGGGATGCAGGAG
                  ************************************************************

HsCD00075304      CTGTTCCGGGGCCACAGCAAGACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCG
NM_032361.3       CTGTTCCGGGGCCACAGCAAGACGCGCGAGTTCCTGGCGCACAGCGCCAAGGTGCACTCG
                  ************************************************************

HsCD00075304      GTGGCCTGGAGTTGCGACGGGCGTCGCCTAGCCTCGGGGTCCTTCGACAAGACGGCCAGC
NM_032361.3       GTGGCCTGGAGTTGCGACGGGCGTCGCCTAGCCTCGGGGTCCTTCGACAAGACGGCCAGC
                  ************************************************************

HsCD00075304      GTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACAATTATCGGGGACATGGGGAT
NM_032361.3       GTCTTCTTGCTGGAGAAGGACCGGTTGGTCAAAGAAAACAATTATCGGGGACATGGGGAT
                  ************************************************************

HsCD00075304      AGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTACGGCGTCTGGA
NM_032361.3       AGTGTGGACCAGCTTTGTTGGCATCCAAGTAATCCTGACCTATTTGTTACGGCGTCCGGA
                  ******************************************************** ***

HsCD00075304      GATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
NM_032361.3       GATAAAACCATTCGCATCTGGGATGTGAGGACTACAAAATGCATTGCCACTGTGAACACT
                  ************************************************************

HsCD00075304      AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAAC
NM_032361.3       AAAGGGGAGAACATTAATATCTGCTGGAGTCCTGATGGGCAGACCATTGCTGTAGGCAAC
                  ************************************************************

HsCD00075304      AAGGATGATGTGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAG
NM_032361.3       AAGGATGATGTGGTGACCTTTATTGATGCCAAGACACACCGTTCCAAAGCAGAAGAGCAG
                  ************************************************************

HsCD00075304      TTCAAGTTCGAGGTCAACGAAATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACA
NM_032361.3       TTCAAGTTCGAGGTCAACGAAATCTCCTGGAACAATGACAATAATATGTTCTTCCTGACA
                  ************************************************************

HsCD00075304      AATGGCAATGGTTGTATCAACATCCTCAGCTACCCAGAACTGAAGCCTGTGCAGTCCATC
NM_032361.3       AATGGCAATGGTTGTATCAACATCCTCAGCTACCCAGAACTGAAGCCTGTGCAGTCCATC
                  ************************************************************

HsCD00075304      AACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACCCCATGGGGAAGTACTTTGCC
NM_032361.3       AACGCCCATCCTTCCAACTGCATCTGTATCAAGTTTGACCCCATGGGGAAGTACTTTGCC
                  ************************************************************

HsCD00075304      ACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGTGTGTGTTCGG
NM_032361.3       ACAGGAAGTGCAGATGCTTTGGTCAGCCTCTGGGATGTGGATGAGTTAGTGTGTGTTCGG
                  ************************************************************

HsCD00075304      TGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
NM_032361.3       TGCTTTTCCAGGCTGGATTGGCCTGTAAGAACCCTCAGTTTCAGCCATGATGGGAAAATG
                  ************************************************************

HsCD00075304      CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAA
NM_032361.3       CTGGCGTCAGCATCGGAAGATCATTTTATTGACATTGCTGAAGTGGAGACAGGGGACAAA
                  ************************************************************

HsCD00075304      CTATGGGAGGTACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCT
NM_032361.3       CTATGGGAGGTACAGTGTGAGTCTCCGACCTTCACAGTGGCGTGGCACCCCAAAAGGCCT
                  ************************************************************

HsCD00075304      CTGCTGGCATTTGCCTGTGATGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGA
NM_032361.3       CTGCTGGCATTTGCCTGTGATGACAAAGACGGCAAATATGACAGCAGCCGGGAAGCCGGA
                  ************************************************************

HsCD00075304      ACTGTGAAGCTGTTTGGGCTTCCTAATGATTCTTGA
NM_032361.3       ACTGTGAAGCTGTTTGGGCTTCCTAATGATTCTTGA
                  ************************************

Protein

SMART domain : WD40 : WD40 repeats
UniProt : Q96J01
HPRD : 09497

Clone

Cloning Information : 251718
HIP Master Clone ID : 31534
Original Clone ID : FLH251718.01X

Organism


TAX_ID : 9606
Species Specific ID: 84321

Genome

Chromosome : 5
Map Location : 5q35.2
Ensembl : ENSG00000051596

Reagents

Labome : THOC3-antibody