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FGF12 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF12
Gene Name:  fibroblast growth factor 12
Sequence             
Original Clone ID: FLH252521.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00075772

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 546nts         Open reading frame : 1 to 546
Reference Sequence Alignment

ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGA
TACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGAC
TACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAGTGAAG
GCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTTTTCACT
CCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACA
CTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAA
ATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCT
ATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAAGGGCGT
TCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAAGATTCA
ACATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 776
Start on reference sequence 231
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF12
Symbol Nomenclature : FGF12
SYNONYM : FGF12B; FHF1
Designation : FGF-12|FHF-1|fibroblast growth factor 12B|fibroblast growth factor FGF-12b|fibroblast growth factor homologous factor 1|myocyte-activating factor
Full Nomenclature : fibroblast growth factor 12
GENEID : 2257
HGNC : 3668
MIM : 601513
Vega : OTTHUMG00000156132
Target GenBank: BC022524

 

Reference Sequence Alignment

  

HsCD00075772        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      --------------ATGAAGATTCTTGAGCTTTCTCTGAAGAAAGGAGTGCATATGCTGC
NM_021032.4         ATGGCTGCGGCGATAGCCAGCTCCTTGATCCGGCAGAAGCGGCAGGCGAGGGAGTCCAAC
                                                                                

HsCD00075772        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      AGTGTCTCTGTGGAAGGAGCCTG-------------------------------------
NM_021032.4         AGCGACCGAGTGTCGGCCTCCAAGCGCCGCTCCAGCCCCAGCAAAGACGGGCGCTCCCTG
                                                                                

HsCD00075772        ------------------------------------------------------------
NM_004113.5         ------------------------------------------------------------
XM_005247227.1      ---------------------------------------------------------AGG
NM_021032.4         TGCGAGAGGCACGTCCTCGGGGTGTTCAGCAAAGTGCGCTTCTGCAGCGGCCGCAAGAGG
                                                                                

HsCD00075772        ------ATGGAGAGCAAAGAACCCCAGCTAAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_004113.5         ------ATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
XM_005247227.1      TCCAGCGACAGCCCAAGTGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
NM_021032.4         CCGGTGAGGCGGAGACCAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAG
                          .   . . .. :***********.******************************

HsCD00075772        CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_004113.5         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
XM_005247227.1      CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
NM_021032.4         CAGGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAAC
                    ************************************************************

HsCD00075772        AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_004113.5         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
XM_005247227.1      AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
NM_021032.4         AGCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGA
                    ************************************************************

HsCD00075772        GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_004113.5         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
XM_005247227.1      GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
NM_021032.4         GTGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTT
                    ************************************************************

HsCD00075772        TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_004113.5         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
XM_005247227.1      TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
NM_021032.4         TTCACTCCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCT
                    ************************************************************

HsCD00075772        TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_004113.5         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
XM_005247227.1      TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
NM_021032.4         TCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAA
                    ************************************************************

HsCD00075772        GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_004113.5         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
XM_005247227.1      GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
NM_021032.4         GGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCG
                    ************************************************************

HsCD00075772        AAACCTATTGAAGTGTGTATGTACAGAGAACAATCGCTACATGAAATTGGAGAAAAACAA
NM_004113.5         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
XM_005247227.1      AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
NM_021032.4         AAACCTATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAA
                    *******************************.****************************

HsCD00075772        GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_004113.5         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
XM_005247227.1      GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
NM_021032.4         GGGCGTTCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAA
                    ************************************************************

HsCD00075772        GATTCAACATAG
NM_004113.5         GATTCAACATAG
XM_005247227.1      GATTCAACATAG
NM_021032.4         GATTCAACATAG
                    ************

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Protein

SMART domain : FGF : Acidic and basic fibroblast growth factor family.
UniProt : P61328
HPRD : 03303

Clone

Cloning Information : 252521
HIP Master Clone ID : 116153
Original Clone ID : FLH252521.01X

Organism


TAX_ID : 9606
Species Specific ID: 2257

Genome

Chromosome : 3
Map Location : 3q28
Ensembl : ENSG00000114279

Reagents

Labome : FGF12-antibody