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STX3A (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  STX3
Gene Name:  syntaxin 3
Sequence             
Original Clone ID: FLH252545.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00075786

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 870nts         Open reading frame : 1 to 870
Reference Sequence Alignment

ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
ATTATTGGACTTTCCGTTGGGCTGAATTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1069
Start on reference sequence 200
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STX3
Symbol Nomenclature : STX3
SYNONYM : STX3A
Designation : syntaxin 3A|syntaxin-3
Full Nomenclature : syntaxin 3
GENEID : 6809
HGNC : 11438
MIM : 600876
Vega : OTTHUMG00000167353
Target GenBank: BC007429

 

Reference Sequence Alignment

  

HsCD00075786        ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
XM_005274195.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
NM_001178040.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
NM_004177.4         ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
XM_005274198.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
                    ************************************************************

HsCD00075786        GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
XM_005274195.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
NM_001178040.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
NM_004177.4         GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
XM_005274198.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
                    ************************************************************

HsCD00075786        GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
XM_005274195.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
NM_001178040.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
NM_004177.4         GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
XM_005274198.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
                    ************************************************************

HsCD00075786        AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
XM_005274195.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
NM_001178040.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
NM_004177.4         AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
XM_005274198.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
                    ************************************************************

HsCD00075786        ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
XM_005274195.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
NM_001178040.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
NM_004177.4         ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
XM_005274198.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
                    ************************************************************

HsCD00075786        CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
XM_005274195.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
NM_001178040.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
NM_004177.4         CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
XM_005274198.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
                    ************************************************************

HsCD00075786        TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
XM_005274195.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
NM_001178040.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
NM_004177.4         TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
XM_005274198.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
                    ************************************************************

HsCD00075786        TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
XM_005274195.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
NM_001178040.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
NM_004177.4         TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
XM_005274198.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
                    ************************************************************

HsCD00075786        ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
XM_005274195.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
NM_001178040.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
NM_004177.4         ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
XM_005274198.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
                    ************************************************************

HsCD00075786        ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
XM_005274195.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
NM_001178040.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
NM_004177.4         ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
XM_005274198.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
                    ************************************************************

HsCD00075786        ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
XM_005274195.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
NM_001178040.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
NM_004177.4         ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
XM_005274198.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
                    ************************************************************

HsCD00075786        CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
XM_005274195.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
NM_001178040.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
NM_004177.4         CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
XM_005274198.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
                    ************************************************************

HsCD00075786        GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
XM_005274195.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
NM_001178040.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
NM_004177.4         GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
XM_005274198.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
                    ************************************************************

HsCD00075786        CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
XM_005274195.1      CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
NM_001178040.1      CGGAAGAAACTGATTTCACTCCAGACTGGTGTGGCCACCCTTGTCTTCAGATGA------
NM_004177.4         CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
XM_005274198.1      CGGAAGAAGAAGATCATGATCATGATCTGCTGTATTATCCTTGCGATCATCTTAGCTTCC
                    ********. :*** :  .**.: .        .  .   *      **  * *      

HsCD00075786        ATTATTGGACTTTCCGTTGGGCTGAATTAG
XM_005274195.1      ATTATTGGACTTTCCGTTGGGCTGAATTAA
NM_001178040.1      ------------------------------
NM_004177.4         ATTATTGGACTTTCCGTTGGGCTGAATTAA
XM_005274198.1      ACCATTGGGAGCATATTTGCCTGA------
                                                  

Pathways

Panther : 5HT1 type receptor mediated signaling pathway
Panther : 5HT2 type receptor mediated signaling pathway
Panther : 5HT3 type receptor mediated signaling pathway
Panther : 5HT4 type receptor mediated signaling pathway
Panther : Beta1 adrenergic receptor signaling pathway
Panther : Beta2 adrenergic receptor signaling pathway
Panther : Beta3 adrenergic receptor signaling pathway
Panther : Cortocotropin releasing factor receptor signaling pathway
Panther : Dopamine receptor mediated signaling pathway
Panther : Opioid prodynorphin pathway
Panther : Opioid proenkephalin pathway
Panther : Opioid proopiomelanocortin pathway
Panther : Oxytocin receptor mediated signaling pathway
Panther : Thyrotropin-releasing hormone receptor signaling pathway

Protein

SMART domain : t_SNARE : Helical region found in SNAREs
SMART domain : SynN : Syntaxin N-terminal domain
UniProt : B4DME0
UniProt : Q53YE2
UniProt : Q13277
HPRD : 15985

Clone

Cloning Information : 252545
HIP Master Clone ID : 28413
Original Clone ID : FLH252545.01X

Organism


TAX_ID : 9606
Species Specific ID: 6809

Genome

Chromosome : 11
Map Location : 11q12.1
Ensembl : ENSG00000166900

Reagents

Labome : STX3-antibody