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CYP39A1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP39A1
Gene Name:  cytochrome P450, family 39, subfamily A, polypeptide 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH190679.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00076244

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1410nts         Open reading frame : 1 to 1410
Reference Sequence Alignment

ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTC
CTTCAGCCGAAGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGA
GTTGGATTTGAGTTTGGGAAAGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTAT
GGACCAATATTTACAGTCTTTGCTATGGGAAACCGAATGACCTTTGTTACTGAAGAAGAA
GGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGATTTTGAACTAGCAGTGCAAAATATC
GTTTATCGTACAGCATCAATTCCAAAGAATGTCTTTTTAGCACTGCATGAAAAACTCTAT
ATTATGTTGAAAGGGAAAATGGGGACTGTCAATCTCCATCAGTTTACTGGGCAACTGACT
GAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTGAACAAC
TTAGTAAGACATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTT
TCCACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGAT
TTTGAGTATGGGTCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAAAAAG
TGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAA
GATAATTCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGAAACAAGTAAG
GAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCT
GTTGCATTTTGGACACTTGCACACGTCCTTTCTCATCCTGATATCCACAAGGCCATTATG
GAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAGGAT
GACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCT
CCTGGTGTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCATTCCT
TCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCT
GAGCCTGAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTC
TTGGACTGCTTCATGGCATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCT
CTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAATATGACTGTAGTCTTCTG
GACCCATTACCCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCGGAAGGGCAA
TGCCGAATTGAATATAAACAAAGAATATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1618
Start on reference sequence 209
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP39A1
Symbol Nomenclature : CYP39A1
Designation : 24-hydroxycholesterol 7-alpha-hydroxylase|cytochrome P450, subfamily XXXIX (oxysterol 7 alpha-hydroxylase), polypeptide 1
Full Nomenclature : cytochrome P450, family 39, subfamily A, polypeptide 1
GENEID : 51302
HGNC : 17449
MIM : 605994
Vega : OTTHUMG00000014785
Target GenBank: BC010358

 

Reference Sequence Alignment

  

HsCD00076244        ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTC
NM_001278738.1      ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTC
NM_016593.4         ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTC
                    ************************************************************

HsCD00076244        CTTCAGCCGAAGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGA
NM_001278738.1      CTTCAGCGGAAGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGA
NM_016593.4         CTTCAGCGGAAGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGA
                    ******* ****************************************************

HsCD00076244        GTTGGATTTGAGTTTGGGAAAGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTAT
NM_001278738.1      GTTGGATTTGAGTTTGGGAAAGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTAT
NM_016593.4         GTTGGATTTGAGTTTGGGAAAGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTAT
                    ************************************************************

HsCD00076244        GGACCAATATTTACAGTCTTTGCTATGGGAAACCGAATGACCTTTGTTACTGAAGAAGAA
NM_001278738.1      GGACCAATATTTACAGTCTTTGCTATGGGAAACCGAATGACCTTTGTTACTGAAGAAGAA
NM_016593.4         GGACCAATATTTACAGTCTTTGCTATGGGAAACCGAATGACCTTTGTTACTGAAGAAGAA
                    ************************************************************

HsCD00076244        GGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGATTTTGAACTAGCAGTGCAAAATATC
NM_001278738.1      GGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGATTTTGAACTAGCAGTGCAAAATATC
NM_016593.4         GGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGATTTTGAACTAGCAGTGCAAAATATC
                    ************************************************************

HsCD00076244        GTTTATCGTACAGCATCAATTCCAAAGAATGTCTTTTTAGCACTGCATGAAAAACTCTAT
NM_001278738.1      GTTTATCGTACAGGG---------------------------------------------
NM_016593.4         GTTTATCGTACAGCATCAATTCCAAAGAATGTCTTTTTAGCACTGCATGAAAAACTCTAT
                    ************* .                                             

HsCD00076244        ATTATGTTGAAAGGGAAAATGGGGACTGTCAATCTCCATCAGTTTACTGGGCAACTGACT
NM_001278738.1      ---------------AAAATGGGGACTGTCAATCTCCATCAGTTTACTGGGCAACTGACT
NM_016593.4         ATTATGTTGAAAGGGAAAATGGGGACTGTCAATCTCCATCAGTTTACTGGGCAACTGACT
                                   *********************************************

HsCD00076244        GAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTGAACAAC
NM_001278738.1      GAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTGAACAAC
NM_016593.4         GAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTGAACAAC
                    ************************************************************

HsCD00076244        TTAGTAAGACATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTT
NM_001278738.1      TTAGTAAGACATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTT
NM_016593.4         TTAGTAAGACATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTT
                    ************************************************************

HsCD00076244        TCCACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGAT
NM_001278738.1      TCCACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGAT
NM_016593.4         TCCACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGAT
                    ************************************************************

HsCD00076244        TTTGAGTATGGGTCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAAAAAG
NM_001278738.1      TTTGAGTATGGGTCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAAAAAG
NM_016593.4         TTTGAGTATGGGTCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAAAAAG
                    ************************************************************

HsCD00076244        TGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAA
NM_001278738.1      TGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAA
NM_016593.4         TGGTTCCTGGAACTGTTTGAGAAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAA
                    ************************************************************

HsCD00076244        GATAATTCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGAAACAAGTAAG
NM_001278738.1      GATAATTCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGAAACAAGTAAG
NM_016593.4         GATAATTCCATGACATTATTGCAAGCTACGCTGGATATTGTAGAGACGGAAACAAGTAAG
                    ************************************************************

HsCD00076244        GAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCT
NM_001278738.1      GAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCT
NM_016593.4         GAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCTTCTCTGTCTAATGCTGTTCCT
                    ************************************************************

HsCD00076244        GTTGCATTTTGGACACTTGCACACGTCCTTTCTCATCCTGATATCCACAAGGCCATTATG
NM_001278738.1      GTTGCATTTTGGACACTTGCATACGTCCTTTCTCATCCTGATATCCACAAGGCCATTATG
NM_016593.4         GTTGCATTTTGGACACTTGCATACGTCCTTTCTCATCCTGATATCCACAAGGCCATTATG
                    ********************* **************************************

HsCD00076244        GAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAGGAT
NM_001278738.1      GAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAGGAT
NM_016593.4         GAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAGGAT
                    ************************************************************

HsCD00076244        GACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCT
NM_001278738.1      GACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCT
NM_016593.4         GACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCT
                    ************************************************************

HsCD00076244        CCTGGTGTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCATTCCT
NM_001278738.1      CCTGGTGTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCATTCCT
NM_016593.4         CCTGGTGTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCATTCCT
                    ************************************************************

HsCD00076244        TCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCT
NM_001278738.1      TCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCT
NM_016593.4         TCTGGTGACTTGTTGATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCT
                    ************************************************************

HsCD00076244        GAGCCTGAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTC
NM_001278738.1      GAGCCTGAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTC
NM_016593.4         GAGCCTGAATTGTTCAAACCTGAACGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTC
                    ************************************************************

HsCD00076244        TTGGACTGCTTCATGGCATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCT
NM_001278738.1      TTGGACTGCTTCATGGCATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCT
NM_016593.4         TTGGACTGCTTCATGGCATTTGGAAGCGGGAAGTTCCAGTGTCCTGCAAGGTGGTTTGCT
                    ************************************************************

HsCD00076244        CTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAATATGACTGTAGTCTTCTG
NM_001278738.1      CTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAATATGACTGTAGTCTTCTG
NM_016593.4         CTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAATATGACTGTAGTCTTCTG
                    ************************************************************

HsCD00076244        GACCCATTACCCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCGGAAGGGCAA
NM_001278738.1      GACCCATTACCCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCGGAAGGGCAA
NM_016593.4         GACCCATTACCCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCGGAAGGGCAA
                    ************************************************************

HsCD00076244        TGCCGAATTGAATATAAACAAAGAATATTG
NM_001278738.1      TGCCGAATTGAATATAAACAAAGAATATGA
NM_016593.4         TGCCGAATTGAATATAAACAAAGAATATGA
                    **************************** .

Pathways

Reactome : Bile acid and bile salt metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Endogenous sterols
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of bile acids and bile salts
Reactome : Synthesis of bile acids and bile salts via 24-hydroxycholesterol
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

UniProt : Q9NYL5
HPRD : 07067

Clone

Cloning Information : 190679
HIP Master Clone ID : 165623
Original Clone ID : FLH190679.01L

Organism


TAX_ID : 9606
Species Specific ID: 51302

Genome

Chromosome : 6
Map Location : 6p21.1-p11.2
Ensembl : ENSG00000146233

Reagents

Labome : CYP39A1-antibody