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ZNF307 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZKSCAN4
Gene Name:  zinc finger with KRAB and SCAN domains 4
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH263702.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00076393

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1638nts         Open reading frame : 1 to 1638
Reference Sequence Alignment

ATGGCTAGAGAACCGAGAAAAAACGCAGCCCTGGACGCCCAGTCTGCAGAAGACCAGACG
GGGCTCCTGACCGTGAAGGTGGAGAAGGAGGAAGCCTCCGCCTTGACGGCGGAGGTGAGA
GCACCCTGCAGCCCTGCTCGGGGCCCCGAACGCTCCCGCCAGCGCTTCCGAGGCTTCCGC
TACCCGGAGGCTGCGGGCCCCCGCGAGGCGTTGAGCCGGCTCCGAGAACTCTGCGGACAA
TGGCTGCAGCCTGAGATGCACAGCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAG
TTCCTGACCATCCTGCCGGGGAATCTGCAGAGCTGGGTGCGGGAGCAGCATCCAGAGAGC
GGGGAGGAGGTGGTGGTGCTATTGGAGTATTTGGAGAGGCAGCTGGATGAGCCGGCGCCG
CAGGTTCCCGTTGGTGACCAGGGGCAAGAACTGCTCTGTTGCAAGATGGCACTATTGACA
CAAACTCAAGGGTCTCAAAGTAGCCAGTGCCAGCCAATGAAGGCTCTGTTCAAGCATGAA
TCTCTGGGATCCCAGCCCTTACACGATAGAGTTCTCCAGGTTCCTGGGCTTGCCCAGGGA
GGGTGCTGCAGAGAAGATGCAATGGTAGCTTCCAGGCTCACTCCAGGGTCCCAGGGTTTG
CTGAAAATGGAAGATGTGGCCCTGACCCTCACTCCTGGGTGGACACAGCTGGATTCATCT
CAGGTGAACCTCTACAGAGATGAAAAGCAGGAGAACCATAGCAGCCTGGTCTCCCTTGGT
GGTGAAATACAGACTAAGAGCAGGGACTTGCCTCCAGTCAAGAAGCTTCCAGAAAAGGAG
CATGGGAAGATATGCCACCTGAGGGAAGACATTGCCCAGATTCCTACACATGCAGAAGCT
GGTGAACAGGAGGGCAGGTTACAAAGAAAGCAGAAAAATGCCATAGGGAGTAGGCGACAT
TATTGCCATGAATGTGGAAAGAGTTTTGCTCAAAGTTCAGGCCTGACTAAACACAGGAGA
ATCCACACTGGTGAGAAACCCTATGAATGTGAAGACTGTGGAAAGACCTTCATTGGGAGC
TCTGCCCTTGTCATTCATCAGAGAGTCCACACTGGTGAGAAGCCATATGAGTGTGAAGAA
TGTGGTAAGGTCTTCAGTCACAGCTCAAACCTTATCAAACACCAGAGAACCCACACTGGG
GAGAAGCCCTATGAGTGTGATGACTGTGGGAAGACCTTCAGCCAGAGCTGCAGCCTCCTT
GAACATCACAAAATTCATACTGGGGAGAAGCCATACCAGTGCAATATGTGTGGCAAAGCC
TTTAGGCGGAATTCACATCTCCTGAGACATCAGAGGATTCATGGTGATAAAAATGTTCAG
AATCCTGAGCACGGGGAGTCCTGGGAAAGTCAGGGTAGGACGGAAAGCCAGTGGGAAAAT
ACTGAGGCTCCCGTGTCTTATAAATGTAATGAGTGTGAGAGAAGTTTCACACGGAATAGA
AGTCTTATTGAACATCAGAAAATCCACACTGGTGAGAAACCCTATCAGTGTGACACATGT
GGAAAAGGTTTCACTCGAACTTCATACCTTGTTCAACATCAGAGAAGCCATGTAGGGAAA
AAAACTCTTTCACAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1882
Start on reference sequence 245
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

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  • Genome
  • Reagents

Gene

 

Gene Symbol : ZKSCAN4
Symbol Nomenclature : ZKSCAN4
SYNONYM : P1P373C6; ZNF307; ZNF427; ZSCAN36; p373c6.1
Designation : p373c6.1 (novel C2H2 type zinc finger protein)|zinc finger protein 307|zinc finger protein 427|zinc finger protein with KRAB and SCAN domains 4
Full Nomenclature : zinc finger with KRAB and SCAN domains 4
GENEID : 387032
HGNC : 13854
MIM : 611643
Vega : OTTHUMG00000014511
Target GenBank: BC014031

 

Reference Sequence Alignment

  

HsCD00076393        ATGGCTAGAGAACCGAGAAAAAACGCAGCCCTGGACGCCCAGTCTGCAGAAGACCAGACG
XM_005249095.1      ATGGCTAGAGAACCGAGAAAAAACGCAGCCCTGGACGCCCAGTCTGCAGAAGACCAGACG
NM_019110.3         ATGGCTAGAGAACCGAGAAAAAACGCAGCCCTGGACGCCCAGTCTGCAGAAGACCAGACG
                    ************************************************************

HsCD00076393        GGGCTCCTGACCGTGAAGGTGGAGAAGGAGGAAGCCTCCGCCTTGACGGCGGAGGTGAGA
XM_005249095.1      GGGCTCCTGACCGTGAAGGTGGAGAAGGAGGAAGCCTCCGCCTTGACGGCGGAGGTGAGA
NM_019110.3         GGGCTCCTGACCGTGAAGGTGGAGAAGGAGGAAGCCTCCGCCTTGACGGCGGAGGTGAGA
                    ************************************************************

HsCD00076393        GCACCCTGCAGCCCTGCTCGGGGCCCCGAACGCTCCCGCCAGCGCTTCCGAGGCTTCCGC
XM_005249095.1      GCACCCTGCAGCCCTGCTCGGGGCCCCGAACGCTCCCGCCAGCGCTTCCGAGGCTTCCGC
NM_019110.3         GCACCCTGCAGCCCTGCTCGGGGCCCCGAACGCTCCCGCCAGCGCTTCCGAGGCTTCCGC
                    ************************************************************

HsCD00076393        TACCCGGAGGCTGCGGGCCCCCGCGAGGCGTTGAGCCGGCTCCGAGAACTCTGCGGACAA
XM_005249095.1      TACCCGGAGGCTGCGGGCCCCCGCGAGGCGTTGAGCCGGCTCCGAGAACTCTGCGGACAA
NM_019110.3         TACCCGGAGGCTGCGGGCCCCCGCGAGGCGTTGAGCCGGCTCCGAGAACTCTGCGGACAA
                    ************************************************************

HsCD00076393        TGGCTGCAGCCTGAGATGCACAGCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAG
XM_005249095.1      TGGCTGCAGCCTGAGATGCACAGCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAG
NM_019110.3         TGGCTGCAGCCTGAGATGCACAGCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAG
                    ************************************************************

HsCD00076393        TTCCTGACCATCCTGCCGGGGAATCTGCAGAGCTGGGTGCGGGAGCAGCATCCAGAGAGC
XM_005249095.1      TTCCTGACCATCCTGCCGGGGAATCTGCAGAGCTGGGTGCGGGAGCAGCATCCAGAGAGC
NM_019110.3         TTCCTGACCATCCTGCCGGGGAATCTGCAGAGCTGGGTGCGGGAGCAGCATCCAGAGAGC
                    ************************************************************

HsCD00076393        GGGGAGGAGGTGGTGGTGCTATTGGAGTATTTGGAGAGGCAGCTGGATGAGCCGGCGCCG
XM_005249095.1      GGGGAGGAGGTGGTGGTGCTATTGGAGTATTTGGAGAGGCAGCTGGATGAGCCGGCGCCG
NM_019110.3         GGGGAGGAGGTGGTGGTGCTATTGGAGTATTTGGAGAGGCAGCTGGATGAGCCGGCGCCG
                    ************************************************************

HsCD00076393        CAGGTTCCCGTTGGTGACCAGGGGCAAGAACTGCTCTGTTGCAAGATGGCACTATTGACA
XM_005249095.1      CAGGTTCCCGTTGGTGACCAGGGGCAAGAACTGCTCTGTTGCAAGATGGCACTATTGACA
NM_019110.3         CAGGTTCCCGTTGGTGACCAGGGGCAAGAACTGCTCTGTTGCAAGATGGCACTATTGACA
                    ************************************************************

HsCD00076393        CAAACTCAAGGGTCTCAAAGTAGCCAGTGCCAGCCAATGAAGGCTCTGTTCAAGCATGAA
XM_005249095.1      CAAACTCAAGGGTCTCAAAGTAGCCAGTGCCAGCCAATGAAGGCTCTGTTCAAGCATGAA
NM_019110.3         CAAACTCAAGGGTCTCAAAGTAGCCAGTGCCAGCCAATGAAGGCTCTGTTCAAGCATGAA
                    ************************************************************

HsCD00076393        TCTCTGGGATCCCAGCCCTTACACGATAGAGTTCTCCAGGTTCCTGGGCTTGCCCAGGGA
XM_005249095.1      TCTCTGGGATCCCAGCCCTTACACGATAGAGTTCTCCAGGTTCCTGGGCTTGCCCAGGGA
NM_019110.3         TCTCTGGGATCCCAGCCCTTACACGATAGAGTTCTCCAGGTTCCTGGGCTTGCCCAGGGA
                    ************************************************************

HsCD00076393        GGGTGCTGCAGAGAAGATGCAATGGTAGCTTCCAGGCTCACTCCAGGGTCCCAGGGTTTG
XM_005249095.1      GGGTGCTGCAGAGAAGATGCAATGGTAGCTTCCAGGCTCACTCCAGGGTCCCAGGGTTTG
NM_019110.3         GGGTGCTGCAGAGAAGATGCAATGGTAGCTTCCAGGCTCACTCCAGGGTCCCAGGGTTTG
                    ************************************************************

HsCD00076393        CTGAAAATGGAAGATGTGGCCCTGACCCTCACTCCTGGGTGGACACAGCTGGATTCATCT
XM_005249095.1      CTGAAAATGGAAGATGTGGCCCTGACCCTCACTCCTGGGTGGACACAGCTGGATTCATCT
NM_019110.3         CTGAAAATGGAAGATGTGGCCCTGACCCTCACTCCTGGGTGGACACAGCTGGATTCATCT
                    ************************************************************

HsCD00076393        CAGGTGAACCTCTACAGAGATGAAAAGCAGGAGAACCATAGCAGCCTGGTCTCCCTTGGT
XM_005249095.1      CAGGTGAACCTCTACAGAGATGAAAAGCAGGAGAACCATAGCAGCCTGGTCTCCCTTGGT
NM_019110.3         CAGGTGAACCTCTACAGAGATGAAAAGCAGGAGAACCATAGCAGCCTGGTCTCCCTTGGT
                    ************************************************************

HsCD00076393        GGTGAAATACAGACTAAGAGCAGGGACTTGCCTCCAGTCAAGAAGCTTCCAGAAAAGGAG
XM_005249095.1      GGTGAAATACAGACTAAGAGCAGGGACTTGCCTCCAGTCAAGAAGCTTCCAGAAAAGGAG
NM_019110.3         GGTGAAATACAGACTAAGAGCAGGGACTTGCCTCCAGTCAAGAAGCTTCCAGAAAAGGAG
                    ************************************************************

HsCD00076393        CATGGGAAGATATGCCACCTGAGGGAAGACATTGCCCAGATTCCTACACATGCAGAAGCT
XM_005249095.1      CATGGGAAGATATGCCACCTGAGGGAAGACATTGCCCAGATTCCTACACATGCAGAAGCT
NM_019110.3         CATGGGAAGATATGCCACCTGAGGGAAGACATTGCCCAGATTCCTACACATGCAGAAGCT
                    ************************************************************

HsCD00076393        GGTGAACAGGAGGGCAGGTTACAAAGAAAGCAGAAAAATGCCATAGGGAGTAGGCGACAT
XM_005249095.1      GGTGAACAGGAGGGCAGGTTACAAAGAAAGCAGAAAAATGCCATAGGGAGTAGGCGACAT
NM_019110.3         GGTGAACAGGAGGGCAGGTTACAAAGAAAGCAGAAAAATGCCATAGGGAGTAGGCGACAT
                    ************************************************************

HsCD00076393        TATTGCCATGAATGTGGAAAGAGTTTTGCTCAAAGTTCAGGCCTGACTAAACACAGGAGA
XM_005249095.1      TATTGCCATGAATGTGGAAAGAGTTTTGCTCAAAGTTCAGGCCTGACTAAACACAGGAGA
NM_019110.3         TATTGCCATGAATGTGGAAAGAGTTTTGCTCAAAGTTCAGGCCTGACTAAACACAGGAGA
                    ************************************************************

HsCD00076393        ATCCACACTGGTGAGAAACCCTATGAATGTGAAGACTGTGGAAAGACCTTCATTGGGAGC
XM_005249095.1      ATCCACACTGGTGAGAAACCCTATGAATGTGAAGACTGTGGAAAGACCTTCATTGGGAGC
NM_019110.3         ATCCACACTGGTGAGAAACCCTATGAATGTGAAGACTGTGGAAAGACCTTCATTGGGAGC
                    ************************************************************

HsCD00076393        TCTGCCCTTGTCATTCATCAGAGAGTCCACACTGGTGAGAAGCCATATGAGTGTGAAGAA
XM_005249095.1      TCTGCCCTTGTCATTCATCAGAGAGTCCACACTGGTGAGAAGCCATATGAGTGTGAAGAA
NM_019110.3         TCTGCCCTTGTCATTCATCAGAGAGTCCACACTGGTGAGAAGCCATATGAGTGTGAAGAA
                    ************************************************************

HsCD00076393        TGTGGTAAGGTCTTCAGTCACAGCTCAAACCTTATCAAACACCAGAGAACCCACACTGGG
XM_005249095.1      TGTGGTAAGGTCTTCAGTCACAGCTCAAACCTTATCAAACACCAGAGAACCCACACTGGG
NM_019110.3         TGTGGTAAGGTCTTCAGTCACAGCTCAAACCTTATCAAACACCAGAGAACCCACACTGGG
                    ************************************************************

HsCD00076393        GAGAAGCCCTATGAGTGTGATGACTGTGGGAAGACCTTCAGCCAGAGCTGCAGCCTCCTT
XM_005249095.1      GAGAAGCCCTATGAGTGTGATGACTGTGGGAAGACCTTCAGCCAGAGCTGCAGCCTCCTT
NM_019110.3         GAGAAGCCCTATGAGTGTGATGACTGTGGGAAGACCTTCAGCCAGAGCTGCAGCCTCCTT
                    ************************************************************

HsCD00076393        GAACATCACAAAATTCATACTGGGGAGAAGCCATACCAGTGCAATATGTGTGGCAAAGCC
XM_005249095.1      GAACATCACAAAATTCATACTGGGGAGAAGCCATACCAGTGCAATATGTGTGGCAAAGCC
NM_019110.3         GAACATCACAAAATTCATACTGGGGAGAAGCCATACCAGTGCAATATGTGTGGCAAAGCC
                    ************************************************************

HsCD00076393        TTTAGGCGGAATTCACATCTCCTGAGACATCAGAGGATTCATGGTGATAAAAATGTTCAG
XM_005249095.1      TTTAGGCGGAATTCACATCTCCTGAGACATCAGAGGATTCATGGTGATAAAAATGTTCAG
NM_019110.3         TTTAGGCGGAATTCACATCTCCTGAGACATCAGAGGATTCATGGTGATAAAAATGTTCAG
                    ************************************************************

HsCD00076393        AATCCTGAGCACGGGGAGTCCTGGGAAAGTCAGGGTAGGACGGAAAGCCAGTGGGAAAAT
XM_005249095.1      AATCCTGAGCACGGGGAGTCCTGGGAAAGTCAGGGTAGGACGGAAAGCCAGTGGGAAAAT
NM_019110.3         AATCCTGAGCACGGGGAGTCCTGGGAAAGTCAGGGTAGGACGGAAAGCCAGTGGGAAAAT
                    ************************************************************

HsCD00076393        ACTGAGGCTCCCGTGTCTTATAAATGTAATGAGTGTGAGAGAAGTTTCACACGGAATAGA
XM_005249095.1      ACTGAGGCTCCCGTGTCTTATAAATGTAATGAGTGTGAGAGAAGTTTCACACGGAATAGA
NM_019110.3         ACTGAGGCTCCCGTGTCTTATAAATGTAATGAGTGTGAGAGAAGTTTCACACGGAATAGA
                    ************************************************************

HsCD00076393        AGTCTTATTGAACATCAGAAAATCCACACTGGTGAGAAACCCTATCAGTGTGACACATGT
XM_005249095.1      AGTCTTATTGAACATCAGAAAATCCACACTGGTGAGAAACCCTATCAGTGTGACACATGT
NM_019110.3         AGTCTTATTGAACATCAGAAAATCCACACTGGTGAGAAACCCTATCAGTGTGACACATGT
                    ************************************************************

HsCD00076393        GGAAAAGGTTTCACTCGAACTTCATACCTTGTTCAACATCAGAGAAGCCATGTAGGGAAA
XM_005249095.1      GGAAAAGGTTTCACTCGAACTTCATACCTTGTTCAACATCAGAGAAGCCATGTAGGGAAA
NM_019110.3         GGAAAAGGTTTCACTCGAACTTCATACCTTGTTCAACATCAGAGAAGCCATGTAGGGAAA
                    ************************************************************

HsCD00076393        AAAACTCTTTCACAGTTG
XM_005249095.1      AAAACTCTTTCACAGTGA
NM_019110.3         AAAACTCTTTCACAGTGA
                    **************** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
SMART domain : SCAN : leucine rich region
UniProt : Q969J2
HPRD : 15768

Clone

Cloning Information : 263702
HIP Master Clone ID : 196490
Original Clone ID : FLH263702.01L

Organism


TAX_ID : 9606
Species Specific ID: 387032

Genome

Chromosome : 6
Map Location : 6p21
Ensembl : ENSG00000187626

Reagents

Labome : ZKSCAN4-antibody