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PSCD3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYTH3
Gene Name:  cytohesin 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH263704.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00076394

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1200nts         Open reading frame : 1 to 1200
Reference Sequence Alignment

ATGGATGAAGACGGCGGCGGCGAGGGTGGTGGCGTGCCTGAAGACCTCTCATTAGAAGAG
AGAGAAGAACTTCTAGACATTCGTCGAAGAAAAAAGGAACTTATTGATGACATTGAGAGG
CTGAAATATGAAATTGCAGAGGTGATGACAGAGATCGACAATCTAACTTCCGTAGAGGAG
AGCAAAACGACTCAGAGGAACAAACAGATAGCCATGGGAAGAAAGAAATTCAACATGGAT
CCCAAAAAGGGAATTCAGTTTCTAATAGAAAATGACCTGCTACAGAGTTCCCCAGAAGAC
GTCGCCCAGTTCCTTTATAAAGGAGAAGGCCTAAATAAGACCGTCATTGGGGACTACCTG
GGTGAAAGGGATGAATTTAATATTAAAGTTCTTCAAGCCTTTGTTGAACTCCATGAGTTT
GCTGATCTCAACCTTGTACAAGCCTTAAGGCAGTTCTTATGGAGCTTCAGGCTGCCCGGG
GAGGCGCAGAAGATTGATCGCATGATGGAGGCTTTCGCTTCTCGCTACTGCCTGTGCAAC
CCCGGGGTCTTCCAGTCCACAGACACGTGCTACGTGCTGTCATTCGCCATCATCATGCTC
AACACCAGCCTCCACAACCACAACGTGCGTGACAAGCCCACGGCAGAACGGTTCATCGCC
ATGAACCGCGGCATCAACGAGGGCGGGGACCTCCCTGAGGAGCTGCTGAGGAATTTGTAT
GAGAGCATTAAGAACGAGCCATTTAAGATCCCGGAGGACGACGGGAACGACCTGACCCAC
ACCTTCTTCAACCCCGACCGCGAGGGCTGGCTCCTGAAGCTGGGAGGGCGTGTGAAGACC
TGGAAGCGCCGGTGGTTCATCCTGACCGATAACTGCCTCTATTACTTTGAATACACAACA
GATAAGGAGCCCAGGGGAATCATCCCGTTGGAAAACCTCAGCATCAGGGAGGTGGAGGAC
CCCCGGAAACCCAACTGTTTTGAGCTCTACAATCCCAGCCACAAAGGGCAGGTCATCAAG
GCCTGTAAGACGGAGGCCGACGGCCGCGTGGTAGAGGGGAACCATGTGGTGTACCGGATC
TCAGCCCCGAGCCCGGAGGAGAAGGAGGAGTGGATGAAATCCATCAAAGCCAGTATCAGC
AGAGATCCCTTCTATGACATGTTGGCAACGAGGAAACGAAGGATTGCCAATAAAAAATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1340
Start on reference sequence 141
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYTH3
Symbol Nomenclature : CYTH3
SYNONYM : ARNO3; GRP1; PSCD3
Designation : ARF nucleotide-binding site opener 3|PH, SEC7 and coiled-coil domain-containing protein 3|cytohesin-3|general receptor of phosphoinositides 1|pleckstrin homology, Sec7 and coiled-coil domains 3
Full Nomenclature : cytohesin 3
GENEID : 9265
Locus Tag : tcag7.847
HGNC : 9504
MIM : 605081
Vega : OTTHUMG00000023440
Target GenBank: BC028717

 

Reference Sequence Alignment

  

HsCD00076394      ATGGATGAAGACGGCGGCGGCGAGGGTGGTGGCGTGCCTGAAGACCTCTCATTAGAAGAG
NM_004227.3       ATGGATGAAGACGGCGGCGGCGAGGGTGGTGGCGTGCCTGAAGACCTCTCATTAGAAGAG
                  ************************************************************

HsCD00076394      AGAGAAGAACTTCTAGACATTCGTCGAAGAAAAAAGGAACTTATTGATGACATTGAGAGG
NM_004227.3       AGAGAAGAACTTCTAGACATTCGTCGAAGAAAAAAGGAACTTATTGATGACATTGAGAGG
                  ************************************************************

HsCD00076394      CTGAAATATGAAATTGCAGAGGTGATGACAGAGATCGACAATCTAACTTCCGTAGAGGAG
NM_004227.3       CTGAAATATGAAATTGCAGAGGTGATGACAGAGATCGACAATCTAACTTCCGTAGAGGAG
                  ************************************************************

HsCD00076394      AGCAAAACGACTCAGAGGAACAAACAGATAGCCATGGGAAGAAAGAAATTCAACATGGAT
NM_004227.3       AGCAAAACGACTCAGAGGAACAAACAGATAGCCATGGGAAGAAAGAAATTCAACATGGAT
                  ************************************************************

HsCD00076394      CCCAAAAAGGGAATTCAGTTTCTAATAGAAAATGACCTGCTACAGAGTTCCCCAGAAGAC
NM_004227.3       CCCAAAAAGGGAATTCAGTTTCTAATAGAAAATGACCTGCTACAGAGTTCCCCAGAAGAC
                  ************************************************************

HsCD00076394      GTCGCCCAGTTCCTTTATAAAGGAGAAGGCCTAAATAAGACCGTCATTGGGGACTACCTG
NM_004227.3       GTCGCCCAGTTCCTTTATAAAGGAGAAGGCCTAAATAAGACCGTCATTGGGGACTACCTG
                  ************************************************************

HsCD00076394      GGTGAAAGGGATGAATTTAATATTAAAGTTCTTCAAGCCTTTGTTGAACTCCATGAGTTT
NM_004227.3       GGTGAAAGGGATGAATTTAATATTAAAGTTCTTCAAGCCTTTGTTGAACTCCATGAGTTT
                  ************************************************************

HsCD00076394      GCTGATCTCAACCTTGTACAAGCCTTAAGGCAGTTCTTATGGAGCTTCAGGCTGCCCGGG
NM_004227.3       GCTGATCTCAACCTTGTACAAGCCTTAAGGCAGTTCTTATGGAGCTTCAGGCTGCCCGGG
                  ************************************************************

HsCD00076394      GAGGCGCAGAAGATTGATCGCATGATGGAGGCTTTCGCTTCTCGCTACTGCCTGTGCAAC
NM_004227.3       GAGGCGCAGAAGATTGATCGCATGATGGAGGCTTTCGCTTCTCGCTACTGCCTGTGCAAC
                  ************************************************************

HsCD00076394      CCCGGGGTCTTCCAGTCCACAGACACGTGCTACGTGCTGTCATTCGCCATCATCATGCTC
NM_004227.3       CCCGGGGTCTTCCAGTCCACAGACACGTGCTACGTGCTGTCATTCGCCATCATCATGCTC
                  ************************************************************

HsCD00076394      AACACCAGCCTCCACAACCACAACGTGCGTGACAAGCCCACGGCAGAACGGTTCATCGCC
NM_004227.3       AACACCAGCCTCCACAACCACAACGTGCGTGACAAGCCCACGGCAGAACGGTTCATCGCC
                  ************************************************************

HsCD00076394      ATGAACCGCGGCATCAACGAGGGCGGGGACCTCCCTGAGGAGCTGCTGAGGAATTTGTAT
NM_004227.3       ATGAACCGCGGCATCAACGAGGGCGGGGACCTCCCTGAGGAGCTGCTGAGGAATTTGTAT
                  ************************************************************

HsCD00076394      GAGAGCATTAAGAACGAGCCATTTAAGATCCCGGAGGACGACGGGAACGACCTGACCCAC
NM_004227.3       GAGAGCATTAAGAACGAGCCATTTAAGATCCCGGAGGACGACGGGAACGACCTGACCCAC
                  ************************************************************

HsCD00076394      ACCTTCTTCAACCCCGACCGCGAGGGCTGGCTCCTGAAGCTGGGAGGGCGTGTGAAGACC
NM_004227.3       ACCTTCTTCAACCCCGACCGCGAGGGCTGGCTCCTGAAGCTGGGAGGGCGTGTGAAGACC
                  ************************************************************

HsCD00076394      TGGAAGCGCCGGTGGTTCATCCTGACCGATAACTGCCTCTATTACTTTGAATACACAACA
NM_004227.3       TGGAAGCGCCGGTGGTTCATCCTGACCGATAACTGCCTCTATTACTTTGAATACACAACA
                  ************************************************************

HsCD00076394      GATAAGGAGCCCAGGGGAATCATCCCGTTGGAAAACCTCAGCATCAGGGAGGTGGAGGAC
NM_004227.3       GATAAGGAGCCCAGGGGAATCATCCCGTTGGAAAACCTCAGCATCAGGGAGGTGGAGGAC
                  ************************************************************

HsCD00076394      CCCCGGAAACCCAACTGTTTTGAGCTCTACAATCCCAGCCACAAAGGGCAGGTCATCAAG
NM_004227.3       CCCCGGAAACCCAACTGTTTTGAGCTCTACAATCCCAGCCACAAAGGGCAGGTCATCAAG
                  ************************************************************

HsCD00076394      GCCTGTAAGACGGAGGCCGACGGCCGCGTGGTAGAGGGGAACCATGTGGTGTACCGGATC
NM_004227.3       GCCTGTAAGACTGAGGCCGACGGCCGCGTGGTAGAGGGGAACCATGTGGTGTACCGGATC
                  *********** ************************************************

HsCD00076394      TCAGCCCCGAGCCCGGAGGAGAAGGAGGAGTGGATGAAATCCATCAAAGCCAGTATCAGC
NM_004227.3       TCAGCCCCGAGCCCGGAGGAGAAGGAGGAGTGGATGAAATCCATCAAAGCCAGTATCAGC
                  ************************************************************

HsCD00076394      AGAGATCCCTTCTATGACATGTTGGCAACGAGGAAACGAAGGATTGCCAATAAAAAATTG
NM_004227.3       AGAGATCCCTTCTATGACATGTTGGCAACGAGGAAACGAAGGATTGCCAATAAAAAATAG
                  **********************************************************:*

Pathways

NCI : Arf6 signaling events
NCI : Class I PI3K signaling events
WikiPathway : Insulin Signaling

Protein

SMART domain : Sec7 : Sec7 domain
SMART domain : PH : Pleckstrin homology domain.
UniProt : O43739
HPRD : 05469

Clone

Cloning Information : 263704
HIP Master Clone ID : 196492
Original Clone ID : FLH263704.01L

Organism


TAX_ID : 9606
Species Specific ID: 9265

Genome

Chromosome : 7
Map Location : 7p22.1
Ensembl : ENSG00000008256

Reagents

Labome : CYTH3-antibody