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ARF6 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARF6
Gene Name:  ADP-ribosylation factor 6
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH020436.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000764

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 528nts         Open reading frame : 1 to 528
Reference Sequence Alignment

ATGGGGAAGGTGCTATCCAAAATCTTCGGGAACAAGGAAATGCGGATCCTCATGTTGGGC
CTGGACGCGGCCGGCAAGACAACAATCCTGTACAAGTTGAAGCTGGGCCAGTCGGTGACC
ACCATTCCCACTGTGGGTTTCAACGTGGAGACGGTGACTTACAAAAATGTCAAGTTCAAC
GTATGGGATGTGGGCGGCCAGGACAAGATCCGGCCGCTCTGGCGGCATTACTACACTGGG
ACCCAAGGTCTCATCTTCGTAGTGGACTGCGCCGACCGCGACCGCATCGATGAGGCTCGC
CAGGAGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATAATCCTCATCTTC
GCCAACAAGCAGGACCTGCCCGATGCCATGAAACCCCACGAGATCCAGGAGAAACTGGGC
CTGACCCGGATTCGGGACAGGAACTGGTATGTGCAGCCCTCCTGTGCCACCTCAGGGGAC
GGACTCTATGAGGGGCTCACATGGTTAACCTCTAACTACAAATCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1045
Start on reference sequence 518
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARF6
Symbol Nomenclature : ARF6
Full Nomenclature : ADP-ribosylation factor 6
GENEID : 382
GI : 61366921
GenBank Accession : AY890991
HGNC : 659
MIM : 600464
Target GenBank: NM_001663

 

Reference Sequence Alignment

  

HsCD00000764      ATGGGGAAGGTGCTATCCAAAATCTTCGGGAACAAGGAAATGCGGATCCTCATGTTGGGC
NM_001663.3       ATGGGGAAGGTGCTATCCAAAATCTTCGGGAACAAGGAAATGCGGATCCTCATGTTGGGC
                  ************************************************************

HsCD00000764      CTGGACGCGGCCGGCAAGACAACAATCCTGTACAAGTTGAAGCTGGGCCAGTCGGTGACC
NM_001663.3       CTGGACGCGGCCGGCAAGACAACAATCCTGTACAAGTTGAAGCTGGGCCAGTCGGTGACC
                  ************************************************************

HsCD00000764      ACCATTCCCACTGTGGGTTTCAACGTGGAGACGGTGACTTACAAAAATGTCAAGTTCAAC
NM_001663.3       ACCATTCCCACTGTGGGTTTCAACGTGGAGACGGTGACTTACAAAAATGTCAAGTTCAAC
                  ************************************************************

HsCD00000764      GTATGGGATGTGGGCGGCCAGGACAAGATCCGGCCGCTCTGGCGGCATTACTACACTGGG
NM_001663.3       GTATGGGATGTGGGCGGCCAGGACAAGATCCGGCCGCTCTGGCGGCATTACTACACTGGG
                  ************************************************************

HsCD00000764      ACCCAAGGTCTCATCTTCGTAGTGGACTGCGCCGACCGCGACCGCATCGATGAGGCTCGC
NM_001663.3       ACCCAAGGTCTCATCTTCGTAGTGGACTGCGCCGACCGCGACCGCATCGATGAGGCTCGC
                  ************************************************************

HsCD00000764      CAGGAGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATAATCCTCATCTTC
NM_001663.3       CAGGAGCTGCACCGCATTATCAATGACCGGGAGATGAGGGACGCCATAATCCTCATCTTC
                  ************************************************************

HsCD00000764      GCCAACAAGCAGGACCTGCCCGATGCCATGAAACCCCACGAGATCCAGGAGAAACTGGGC
NM_001663.3       GCCAACAAGCAGGACCTGCCCGATGCCATGAAACCCCACGAGATCCAGGAGAAACTGGGC
                  ************************************************************

HsCD00000764      CTGACCCGGATTCGGGACAGGAACTGGTATGTGCAGCCCTCCTGTGCCACCTCAGGGGAC
NM_001663.3       CTGACCCGGATTCGGGACAGGAACTGGTATGTGCAGCCCTCCTGTGCCACCTCAGGGGAC
                  ************************************************************

HsCD00000764      GGACTCTATGAGGGGCTCACATGGTTAACCTCTAACTACAAATCTTTG
NM_001663.3       GGACTCTATGAGGGGCTCACATGGTTAACCTCTAACTACAAATCTTAA
                  **********************************************:.

Pathways

NCI : Alpha4 beta1 integrin signaling events
NCI : Arf6 downstream pathway
NCI : Arf6 signaling events
NCI : Arf6 trafficking events
NCI : Class I PI3K signaling events
NCI : E-cadherin signaling in the nascent adherens junction
NCI : Plexin-D1 Signaling
NCI : Posttranslational regulation of adherens junction stability and dissassembly
NCI : Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI : Stabilization and expansion of the E-cadherin adherens junction
Panther : Huntington disease
Panther : Integrin signalling pathway
WikiPathway : EGF/EGFR Signaling Pathway
WikiPathway : Insulin Signaling

Clone

Cloning Information : 20436
HIP Master Clone ID : 36782
Original Clone ID : FLH020436.01L

Organism


TAX_ID : 9606
Species Specific ID: 382

Genome

Chromosome : 14
Map Location : 14q21.3

Reagents

Labome : ARF6-antibody