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CHRNA5 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CHRNA5
Gene Name:  cholinergic receptor, nicotinic, alpha 5 (neuronal)
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH226425.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00077516

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1407nts         Open reading frame : 1 to 1407
Reference Sequence Alignment

ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC
GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT
TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA
TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA
TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA
CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT
ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT
GGACGTTTTGAAGGGACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACCTGG
ACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTTGAC
CTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGATATA
ATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAGATT
GTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGGTATCCGTATGTC
ACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTTATAATACCC
TGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAATGAAGGTGAA
AAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTGGTTATTGAA
GAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTGGTATTTACC
ATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATTCATCATCGT
TCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTTCACACGCTT
CCCAAACTGCTTTGCATGAGAAGTCATGTAGACAGGTACTTCACTCAGAAAGAGGAAACT
GAGAGTGGTAGTGGACCAAAATCTTCTAGAAACACATTGGAAGCTGCGCTCGATTCTATT
CGCTACATTACAAGACACATCATGAAGGAAAATGATGTCCGTGAGGTTGTTGAAGATTGG
AAATTCATAGCCCAGGTTCTTGATCGGATGTTTCTGTGGACTTTTCTTTTCGTTTCAATT
GTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTATAAATGGGCAAATATATTAATACCA
GTTCATATTGGAAATGCAAATAAGTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1569
Start on reference sequence 163
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CHRNA5
Symbol Nomenclature : CHRNA5
SYNONYM : LNCR2
Designation : Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5|acetylcholine receptor, nicotinic, alpha 5 (neuronal)|neuronal acetylcholine receptor subunit alpha-5|neuronal nicotinic acetylcholine receptor, alpha5 subunit
Full Nomenclature : cholinergic receptor, nicotinic, alpha 5 (neuronal)
GENEID : 1138
HGNC : 1959
MIM : 118505
Vega : OTTHUMG00000143858
Target GenBank: BC033639

 

Reference Sequence Alignment

  

HsCD00077516        ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC
NM_000745.3         ATGGCGGCGCGGGGGTCAGGGCCCCGCGCGCTCCGCCTGCTGCTCTTGGTCCAGCTGGTC
NM_001166694.1      -----------ATGGGCTCTGGCCCGCTCTCGCTGCCCCTGGCGCTGTCGCCGCCGCGGC
                               . ** *:  * ***** * * * ***    ** **    **. *  * *

HsCD00077516        GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT
NM_000745.3         GCGGGGCGCTGCGGTCTAGCGGGCGCGGCGGGCGGCGCGCAGAGAGGATTATCTGAACCT
NM_001166694.1      TGCTGCTGCTGCTG--CTGCTGTCTCTGCTGCCAGTGGCCAGG-----------------
                        *  ***** *   :** * * * ** * *.* *  ***.                 

HsCD00077516        TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA
NM_000745.3         TCTTCTATTGCAAAACATGAAGATAGTTTGCTTAAGGATTTATTTCAAGACTACGAAAGA
NM_001166694.1      ------GCCTCAGAGGCTGAGCACCGTCTATTTGAGCGGCTGTTTGAAGATTACAATGAG
                          .   **.*. .***. * .** *. **.** .  *.*** **** ***.*:...

HsCD00077516        TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA
NM_000745.3         TGGGTTCGTCCTGTGGAACACCTGAATGACAAAATAAAAATAAAATTTGGACTTGCAATA
NM_001166694.1      ATCATCCGGCCTGTAGCCAACGTGTCTGACCCAGTCATCATCCATTTCGAGGTGTCCATG
                    :  .* ** *****.*...** **:.****..*.*.*:.**..*:** *.. *  *.**.

HsCD00077516        TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA
NM_000745.3         TCTCAATTGGTGGATGTGGATGAGAAAAATCAGTTAATGACAACAAACGTCTGGTTGAAA
NM_001166694.1      TCTCAGCTGGTGAAGGTGGATGAAGTAAACCAGATCATGGAGACCAACCTGTGGCTCAAG
                    *****. *****.* ********..:*** ***:*.***...**.*** * *** * **.

HsCD00077516        CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT
NM_000745.3         CAGGAATGGATAGATGTAAAATTAAGATGGAACCCTGATGACTATGGTGGAATAAAAGTT
NM_001166694.1      CAAATCTGGAATGACTACAAGCTGAAATGGAACCCCTCTGACTATGGTGGGGCAGAGTTC
                    **..:.****::**  :.**. *.*.*********  .************.. *.*. * 

HsCD00077516        ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT
NM_000745.3         ATACGTGTTCCTTCAGACTCTGTCTGGACACCAGACATCGTTTTGTTTGATAATGCAGAT
NM_001166694.1      ATGCGTGTCCCTGCACAGAAGATCTGGAAGCCAGACATTGTGCTGTATAACAATGCTGTT
                    **.***** *** ** * :. .******..******** **  ***:*.* *****:*:*

HsCD00077516        GGACGTTTTGAAGGG---ACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACC
NM_000745.3         GGACGTTTTGAAGGG---ACCAGTACGAAAACAGTCATCAGGTACAATGGCACTGTCACC
NM_001166694.1      GGGGATTTCCAGGTGGACGACAAGACCAAAGCCTTACTCAAGTACACTGGGGAGGTGACT
                    **. .***  *.* *   ..**. ** ***.*. *..***.*****.*** .. ** ** 

HsCD00077516        TGGACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTT
NM_000745.3         TGGACTCCACCGGCAAACTACAAAAGTTCCTGTACCATAGATGTCACGTTTTTCCCATTT
NM_001166694.1      TGGATACCTCCGGCCATCTTTAAGAGCTCCTGTAAAATCGACGTGACCTACTTCCCGTTT
                    **** :**:*****.*:**: **.** *******..**.** ** ** *: *****.***

HsCD00077516        GACCTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGAT
NM_000745.3         GACCTTCAGAACTGTTCCATGAAATTTGGTTCTTGGACTTATGATGGATCACAGGTTGAT
NM_001166694.1      GATTACCAAAACTGTACCATGAAGTTCGGTTCCTGGTCCTACGATAAGGCGAAAATCGAT
                    **  : **.******:*******.** ***** ***:* ** ***... *..*..* ***

HsCD00077516        ATAATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAG
NM_000745.3         ATAATTCTAGAGGACCAAGATGTAGACAAGAGAGATTTTTTTGATAATGGAGAATGGGAG
NM_001166694.1      CTGGTCCTGATCGGCTCTTCCATGAACCTCAAGGACTATTGGGAGAGCGGCGAGTGGGCC
                    .*..* **..: *.* .: . .*..**.: *..** *:**  ** *. **.**.****. 

HsCD00077516        ATTGTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGG------TAT
NM_000745.3         ATTGTGAGTGCAACAGGGAGCAAAGGAAACAGAACCGACAGCTGTTGCTGG------TAT
NM_001166694.1      ATCATCAAAGCCCCAGGCTACAAACACGACATCAAGTACAACTGCTGCGAGGAGATCTAC
                    ** .* *.:**..**** :.**** ...*** .*.  ***.*** *** .*      ** 

HsCD00077516        CCGTATGTCACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTT
NM_000745.3         CCGTATGTCACTTACTCATTTGTAATCAAGCGCCTGCCTCTCTTTTATACCTTGTTCCTT
NM_001166694.1      CCCGACATCACATACTCGCTGTACATCCGGCGCCTGCCCTTGTTCTACACCATCAACCTC
                    **  * .****:*****. *  :.***..*********  * ** ** ***:* ::*** 

HsCD00077516        ATAATACCCTGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAAT
NM_000745.3         ATAATACCCTGTATTGGGCTCTCATTTTTAACTGTACTTGTCTTCTATCTTCCTTCAAAT
NM_001166694.1      ATCATCCCCTGCCTGCTCATCTCCTTCCTCACTGTGCTCGTCTTCTACCTGCCCTCCGAC
                    **.**.***** .*    .****.**  *.*****.** ******** ** ** **..* 

HsCD00077516        GAAGGTGAAAAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTG
NM_000745.3         GAAGGTGAAAAGATTTGTCTCTGCACTTCAGTACTTGTGTCTTTGACTGTCTTCCTTCTG
NM_001166694.1      TGCGGTGAGAAGGTGACCCTGTGCATTTCTGTCCTCCTCTCCCTGACGGTGTTTCTCCTG
                     ..*****.***.* :  ** **** ***:**.**  * **  **** ** ** ** ***

HsCD00077516        GTTATTGAAGAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTG
NM_000745.3         GTTATTGAAGAGATCATACCATCATCTTCAAAAGTCATACCTCTAATTGGAGAGTATCTG
NM_001166694.1      GTGATCACTGAGACCATCCCTTCCACCTCGCTGGTCATCCCCCTGATTGGAGAGTACCTC
                    ** ** ..:**** ***.**:**.:* **..:.*****.** **.*********** ** 

HsCD00077516        GTATTTACCATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATT
NM_000745.3         GTATTTACCATGATTTTTGTGACACTGTCAATTATGGTAACCGTCTTCGCTATCAACATT
NM_001166694.1      CTGTTCACCATGATTTTTGTAACCTTGTCCATCGTCATCACCGTCTTCGTGCTCAACGTG
                     *.** **************.**. ****.** .* .*.**********  .*****.* 

HsCD00077516        CATCATCGTTCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTT
NM_000745.3         CATCATCGTTCTTCCTCAACACATAATGCCATGGCGCCTTTGGTCCGCAAGATATTTCTT
NM_001166694.1      CACTACAG---AACCCCGACGACACACACAATGCCCTCATGGGTGAAGACTGTATTCTTG
                    **  * .*   ::** *.**...:.* .*.*** *  *:* *** .. *. .****  * 

HsCD00077516        CACACGCTTCCCAAACTGCTTTGCATGAGAAGTCATGTAG---ACAGGTACTTCACTCAG
NM_000745.3         CACACGCTTCCCAAACTGCTTTGCATGAGAAGTCATGTAG---ACAGGTACTTCACTCAG
NM_001166694.1      AACCTGCTCCCCAGGGTCATGTTCATGACCAGGCCAACAAGCAACGAGGGCAACGCTCAG
                    .**. *** ****.. * .* * ***** .** *.:. *.   **..* .*::*.*****

HsCD00077516        AAAGAGGAAACTGAGAGTGGTAGTGG-------------ACCAAAATCTTCTAGAAACAC
NM_000745.3         AAAGAGGAAACTGAGAGTGGTAGTGG-------------ACCAAAATCTTCTAGAAACAC
NM_001166694.1      AAGCCGAGGCCCCTCTACGGTGCCGAGCTCTCAAATCTGAATTGCTTCAGCCGCGCAGAG
                    **. .*....*  : :. ***.  *.             *. :..:**: * . ..* * 

HsCD00077516        ATTGGAAGCTGCGCTCGATTCTATTCGCTACATTACAAGACACATCATGAAGGAAAATGA
NM_000745.3         ATTGGAAGCTGCGCTCGATTCTATTCGCTACATTACAAGACACATCATGAAGGAAAATGA
NM_001166694.1      TCCAAAGGCTGCAAGGAGGGCTACCCCTGCCAGGACGGGATGTGTGGTTACTGCCACCAC
                    :  ..*.*****..  ..  ***  *   .**  **..** . .* .* *. *..*. ..

HsCD00077516        TGTCCGTGAGGTTGTTGAAGATTGGAAATTCATAGCCCAGGTTCTTGATCGGATGTTTCT
NM_000745.3         TGTCCGTGAGGTTGTTGAAGATTGGAAATTCATAGCCCAGGTTCTTGATCGGATGTTTCT
NM_001166694.1      CGCAGGATAAAAATCTCCAATTTCAGTGCTAACCTCACGAGAAGCTCTAGTTCTGAATCT
                     * . *: *..::  * .*.:** ..:. *.* . *.*..*::  * ::   .**::***

HsCD00077516        GTGGACTTTTCTTTTCGTTTCAATTGTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTA
NM_000745.3         GTGGACTTTTCTTTTCGTTTCAATTGTTGGATCTCTTGGGCTTTTTGTTCCTGTTATTTA
NM_001166694.1      GTTGATGCTGTGCTGTCCCTCTCTGCTTTGTCACCAGAAATCAAAGAAGCCATCCAAAGT
                    ** **   *    *     **:.*  ** *: . *: ...  ::: .: **:   *:: :

HsCD00077516        TAAATGGGCAAATATATTAATACCAGTTCATATTGGAAATGCAAATAAGTTG--------
NM_000745.3         TAAATGGGCAAATATATTAATACCAGTTCATATTGGAAATGCAAATAAGTGA--------
NM_001166694.1      GTCAAGTATATTGCTGAAAATATGAAAGCACAAAATGAAGCCAAAGAGGAACAAAAAGCC
                     :.*:* . *:: .*.::****  *.: ** *::. .**  **** *.*:          

HsCD00077516        ------------------------------------------------------------
NM_000745.3         ------------------------------------------------------------
NM_001166694.1      CAAGAGATCCAACAATTGAAACGAAAAGAAAAGTCCACAGAAACATCCGATCAAGAACCT
                                                                                

HsCD00077516        ---------
NM_000745.3         ---------
NM_001166694.1      GGGCTATGA
                             

Pathways

Panther : Nicotine pharmacodynamics pathway
Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Acetylcholine Binding And Downstream Events
Reactome : Activation of Nicotinic Acetylcholine Receptors
Reactome : Highly calcium permeable nicotinic acetylcholine receptors
Reactome : Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Postsynaptic nicotinic acetylcholine receptors
Reactome : Presynaptic nicotinic acetylcholine receptors
Reactome : Transmission across Chemical Synapses
WikiPathway : Nicotine Activity on Dopaminergic Neurons

Protein

UniProt : P30532
UniProt : Q6EWN4
HPRD : 00334

Clone

Cloning Information : 226425
HIP Master Clone ID : 171248
Original Clone ID : FLH226425.01L

Organism


TAX_ID : 9606
Species Specific ID: 1138

Genome

Chromosome : 15
Map Location : 15q24
Ensembl : ENSG00000169684

Reagents

Labome : CHRNA5-antibody