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CD83 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CD83
Gene Name:  CD83 molecule
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH225112.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00078431

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 618nts         Open reading frame : 1 to 618
Reference Sequence Alignment

ATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCCCGCGACG
CCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCCCTGGGAT
CCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGAGAGGATG
GAGACACCCCAGGAAGACCACCTCAGGGGACAGCACTATCATCAGAAGGGGCAAAATGGT
TCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTACCAGCTGC
AACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCTAAGTGGC
AAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTTTAAGAAA
TACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACTCATCATT
TTCACTTGTAAGTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGCTGGCATG
GAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGACTCCTCAC
AAGACAGAACTGGTATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 750
Start on reference sequence 133
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CD83
Symbol Nomenclature : CD83
SYNONYM : BL11; HB15
Designation : B-cell activation protein|CD83 antigen|CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)|cell surface protein HB15|cell-surface glycoprotein|hCD83
Full Nomenclature : CD83 molecule
GENEID : 9308
HGNC : 1703
MIM : 604534
Vega : OTTHUMG00000014283
Target GenBank: BC030830

 

Reference Sequence Alignment

  

HsCD00078431        ATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCCCGCGACG
NM_004233.3         ATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCCCGCGACG
NM_001040280.1      ATGTCGCGCGGCCTCCAGCTTCTGCTCCTGAGCTGCGCCTACAGCCTGGCTCCCGCGACG
                    ************************************************************

HsCD00078431        CCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCCCTGGGAT
NM_004233.3         CCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCCCTGGGAT
NM_001040280.1      CCGGAGGTGAAGGTGGCTTGCTCCGAAGATGTGGACTTGCCCTGCACCGCCCCCTGGGAT
                    ************************************************************

HsCD00078431        CCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGAGAGGATG
NM_004233.3         CCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGAGAGGATG
NM_001040280.1      CCGCAGGTTCCCTACACGGTCTCCTGGGTCAAGTTATTGGAGGGTGGTGAAGAGAGGATG
                    ************************************************************

HsCD00078431        GAGACACCCCAGGAAGACCACCTCAGGGGACAGCACTATCATCAGAAGGGGCAAAATGGT
NM_004233.3         GAGACACCCCAGGAAGACCACCTCAGGGGACAGCACTATCATCAGAAGGGGCAAAATGGT
NM_001040280.1      GAGACACCCCAGGAAGACCACCTCAGGGGACAGCACTATCATCAGAAGGGGCAAAATGGT
                    ************************************************************

HsCD00078431        TCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTACCAGCTGC
NM_004233.3         TCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTACCAGCTGC
NM_001040280.1      TCTTTCGACGCCCCCAATGAAAGGCCCTATTCCCTGAAGATCCGAAACACTACCAGCTGC
                    ************************************************************

HsCD00078431        AACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCTAAGTGGC
NM_004233.3         AACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCTAAGTGGC
NM_001040280.1      AACTCGGGGACATACAGGTGCACTCTGCAGGACCCGGATGGGCAGAGAAACCTAAGTGGC
                    ************************************************************

HsCD00078431        AAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTTTAAGAAA
NM_004233.3         AAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTTTAAGAAA
NM_001040280.1      AAGGTGATCTTGAGAGTGACAGGATGCCCTGCACAGCGTAAAGAAGAGACTTTTAAGAAA
                    ************************************************************

HsCD00078431        TACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACTCATCATT
NM_004233.3         TACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACTCATCATT
NM_001040280.1      TACAGAGCGGAGATTGTCCTGCTGCTGGCTCTGGTTATTTTCTACTTAACACTCATCATT
                    ************************************************************

HsCD00078431        TTCACTTGTAAGTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGCTGGCATG
NM_004233.3         TTCACTTGTAAGTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGCTGGCATG
NM_001040280.1      TTCACTTG---TTTTGCACGGCTACAGAGTATCTTCCCAGATTTTTCTAAAGCTGGCATG
                    ********    ************************************************

HsCD00078431        GAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGACTCCTCAC
NM_004233.3         GAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGACTCCTCAC
NM_001040280.1      GAACGAGCTTTTCTCCCAGTTACCTCCCCAAATAAGCATTTAGGGCTAGTGACTCCTCAC
                    ************************************************************

HsCD00078431        AAGACAGAACTGGTATTG
NM_004233.3         AAGACAGAACTGGTATGA
NM_001040280.1      AAGACAGAACTGGTATGA
                    **************** .

Pathways

WikiPathway : TCR Signaling Pathway

Protein

SMART domain : IG : Immunoglobulin
UniProt : Q01151
HPRD : 05170

Clone

Cloning Information : 225112
HIP Master Clone ID : 160434
Original Clone ID : FLH225112.01L

Organism


TAX_ID : 9606
Species Specific ID: 9308

Genome

Chromosome : 6
Map Location : 6p23
Ensembl : ENSG00000112149

Reagents

Labome : CD83-antibody