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LYSMD2 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  LYSMD2
Gene Name:  LysM, putative peptidoglycan-binding, domain containing 2
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH225140.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00078455

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 648nts        
Open reading frame : 1 to 648
Reference Sequence Alignment

ATGGCGGATTCCTCGCCCGCACTGTCCCTGCGGGAAGGCGGCCCCCGCGCGCCGCGGCCC
TCGGCCCCCTCGCCGCCGCCGCGCTCGCGCTCCGGCTCCGAGTCCGAGGAGGCCGAGCTG
TCGCTGAGCCTGGCCCGCACCAAGACCCGCTCGTACGGCAGCACCGCCAGCGTGCGGGCG
CCGCTGGGCGCCGGCGTCATCGAGCGCCATGTGGAGCACCGGGTCCGCGCGGGCGACACG
CTGCAGGGCATCGCGCTCAAGTACGGTGTCACGATGGAACAGATTAAAAGGGCCAATAAA
CTGTTTACCAATGATTGTATATTTCTGAAGAAAACTTTGAACATCCCAGTTATATCAGAG
AAGCCTTTGTTGTTTAATGGACTTAACTCCATTGATTCTCCAGAAAATGAAACTGCTGAT
AACAGTTTTTCTCAGGAAGAGGAGCCAGTGGTGGCCGGGGAAGACCTCCCTCCTCCCAGT
CCTCAAGAATCTGATGTTCAGCCTGTGCAGCCTGAGGAAGTGTCAGCCAGAGATTTCCTG
CAGAGACTTGACTTGCAGATTAAGTTATCAACACAGGCAGCCAAGAAGCTAAAAGAAGAG
AGCAGAGATGAAGAAAGTCCCTATGCAACTTCCCTCTATCACAGTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1146
Start on reference sequence 499
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Al Lau
Niroshan Ramachandran
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR1 AttR1 site for recombination in empty vector 549 565
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LYSMD2
Symbol Nomenclature : LYSMD2
Designation : lysM and putative peptidoglycan-binding domain-containing protein 2
Full Nomenclature : LysM, putative peptidoglycan-binding, domain containing 2
GENEID : 256586
HGNC : 28571
Vega : OTTHUMG00000131804
Target GenBank: BC033515

 

Reference Sequence Alignment

  

HsCD00078455      ATGGCGGATTCCTCGCCCGCACTGTCCCTGCGGGAAGGCGGCCCCCGCGCGCCGCGGCCC
NM_153374.2       ATGGCGGATTCCTCGCCCGCACTGTCCCTGCGGGAAGGCGGCCCCCGCGCGCCGCGGCCC
                  ************************************************************

HsCD00078455      TCGGCCCCCTCGCCGCCGCCGCGCTCGCGCTCCGGCTCCGAGTCCGAGGAGGCCGAGCTG
NM_153374.2       TCGGCCCCCTCGCCGCCGCCGCGCTCGCGCTCCGGCTCCGAGTCCGAGGAGGCCGAGCTG
                  ************************************************************

HsCD00078455      TCGCTGAGCCTGGCCCGCACCAAGACCCGCTCGTACGGCAGCACCGCCAGCGTGCGGGCG
NM_153374.2       TCGCTGAGCCTGGCCCGCACCAAGACCCGCTCGTACGGCAGCACCGCCAGCGTGCGGGCG
                  ************************************************************

HsCD00078455      CCGCTGGGCGCCGGCGTCATCGAGCGCCATGTGGAGCACCGGGTCCGCGCGGGCGACACG
NM_153374.2       CCGCTGGGCGCCGGCGTCATCGAGCGCCATGTGGAGCACCGGGTCCGCGCGGGCGACACG
                  ************************************************************

HsCD00078455      CTGCAGGGCATCGCGCTCAAGTACGGTGTCACGATGGAACAGATTAAAAGGGCCAATAAA
NM_153374.2       CTGCAGGGCATCGCGCTCAAGTACGGTGTCACGATGGAACAGATTAAAAGGGCCAATAAA
                  ************************************************************

HsCD00078455      CTGTTTACCAATGATTGTATATTTCTGAAGAAAACTTTGAACATCCCAGTTATATCAGAG
NM_153374.2       CTGTTTACCAATGATTGTATATTTCTGAAGAAAACTTTGAACATCCCAGTTATATCAGAG
                  ************************************************************

HsCD00078455      AAGCCTTTGTTGTTTAATGGACTTAACTCCATTGATTCTCCAGAAAATGAAACTGCTGAT
NM_153374.2       AAGCCTTTGTTGTTTAATGGACTTAACTCCATTGATTCTCCAGAAAATGAAACTGCTGAT
                  ************************************************************

HsCD00078455      AACAGTTTTTCTCAGGAAGAGGAGCCAGTGGTGGCCGGGGAAGACCTCCCTCCTCCCAGT
NM_153374.2       AACAGTTTTTCTCAGGAAGAGGAGCCAGTGGTGGCCGGGGAAGACCTCCCTCCTCCCAGT
                  ************************************************************

HsCD00078455      CCTCAAGAATCTGATGTTCAGCCTGTGCAGCCTGAGGAAGTGTCAGCCAGAGATTTCCTG
NM_153374.2       CCTCAAGAATCTGATGTTCAGCCTGTGCAGCCTGAGGAAGTGTCAGCCAGAGATTTCCTG
                  ************************************************************

HsCD00078455      CAGAGACTTGACTTGCAGATTAAGTTATCAACACAGGCAGCCAAGAAGCTAAAAGAAGAG
NM_153374.2       CAGAGACTTGACTTGCAGATTAAGTTATCAACACAGGCAGCCAAGAAGCTAAAAGAAGAG
                  ************************************************************

HsCD00078455      AGCAGAGATGAAGAAAGTCCCTATGCAACTTCCCTCTATCACAGTTTG
NM_153374.2       AGCAGAGATGAAGAAAGTCCCTATGCAACTTCCCTCTATCACAGTTAG
                  **********************************************:*

Protein

SMART domain : LysM : Lysin motif
UniProt : Q8IV50
HPRD : 17531

Clone

Cloning Information : 225140
HIP Master Clone ID : 161154
Original Clone ID : FLH225140.01L

Organism


TAX_ID : 9606
Species Specific ID: None

Genome

Chromosome : 15
Map Location : 15q21.2
Ensembl : ENSG00000140280

Reagents

Labome : LYSMD2-antibody