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CAPN6 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN6
Gene Name:  calpain 6
Sequence              Map: pANT7_cGST.doc
Original Clone ID: FLH224675.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00079240

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1926nts         Open reading frame : 1 to 1926
Reference Sequence Alignment

ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
CTCTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1955
Start on reference sequence 30
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN6
Symbol Nomenclature : CAPN6
SYNONYM : CANPX; CAPNX; CalpM; DJ914P14.1
Designation : calpain-6|calpain-like protease X-linked|calpamodulin
Full Nomenclature : calpain 6
GENEID : 827
HGNC : 1483
MIM : 300146
Vega : OTTHUMG00000022203
Target GenBank: BC000730

 

Reference Sequence Alignment

  

HsCD00079240      ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
NM_014289.3       ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
                  ************************************************************

HsCD00079240      ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
NM_014289.3       ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
                  ************************************************************

HsCD00079240      TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
NM_014289.3       TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
                  ************************************************************

HsCD00079240      CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
NM_014289.3       CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
                  ************************************************************

HsCD00079240      AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
NM_014289.3       AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
                  ************************************************************

HsCD00079240      ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
NM_014289.3       ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
                  ************************************************************

HsCD00079240      TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
NM_014289.3       TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
                  ************************************************************

HsCD00079240      CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
NM_014289.3       CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
                  ************************************************************

HsCD00079240      GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
NM_014289.3       GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
                  ************************************************************

HsCD00079240      ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
NM_014289.3       ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
                  ************************************************************

HsCD00079240      AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
NM_014289.3       AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
                  ************************************************************

HsCD00079240      ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
NM_014289.3       ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
                  ************************************************************

HsCD00079240      GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
NM_014289.3       GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
                  ************************************************************

HsCD00079240      AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
NM_014289.3       AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
                  ************************************************************

HsCD00079240      CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
NM_014289.3       CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
                  ************************************************************

HsCD00079240      GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
NM_014289.3       GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
                  ************************************************************

HsCD00079240      GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
NM_014289.3       GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
                  ************************************************************

HsCD00079240      CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
NM_014289.3       CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
                  ************************************************************

HsCD00079240      ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
NM_014289.3       ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
                  ************************************************************

HsCD00079240      TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
NM_014289.3       TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
                  ************************************************************

HsCD00079240      TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
NM_014289.3       TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
                  ************************************************************

HsCD00079240      ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
NM_014289.3       ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
                  ************************************************************

HsCD00079240      ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
NM_014289.3       ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
                  ************************************************************

HsCD00079240      TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
NM_014289.3       TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
                  ************************************************************

HsCD00079240      GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
NM_014289.3       GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
                  ************************************************************

HsCD00079240      ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
NM_014289.3       ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
                  ************************************************************

HsCD00079240      ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
NM_014289.3       ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
                  ************************************************************

HsCD00079240      TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
NM_014289.3       TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
                  ************************************************************

HsCD00079240      GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
NM_014289.3       GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
                  ************************************************************

HsCD00079240      CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
NM_014289.3       CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
                  ************************************************************

HsCD00079240      GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
NM_014289.3       GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
                  ************************************************************

HsCD00079240      GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
NM_014289.3       GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
                  ************************************************************

HsCD00079240      CTCTTG
NM_014289.3       CTCTAA
                  ****:.

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 224675
HIP Master Clone ID : 155703
Original Clone ID : FLH224675.01L

Organism


TAX_ID : 9606
Species Specific ID: 827

Genome

Chromosome : X
Map Location : Xq23
Ensembl : ENSG00000077274

Reagents

Labome : CAPN6-antibody