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LOC146542 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF688
Gene Name:  zinc finger protein 688
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH257058.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079552

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 831nts         Open reading frame : 1 to 831
Reference Sequence Alignment

ATGGCGCCGCCCCCAGCCCCGCTCCTGGCGCCGAGGCCCGGGGAGACCCGGCCTGGTTGC
AGGAAGCCCGGGACTGTGAGCTTCGCGGACGTGGCCGTGTACTTCTCCCCGGAGGAGTGG
GGCTGTCTGCGGCCCGCGCAGAGGGCTCTGTACCGGGACGTGATGCAGGAGACCTACGGC
CACCTGGGCGCGCTCGGATTCCCAGGCCCCAAACCAGCCCTCATCTCTTGGATGGAACAG
GAGAGTGAGGCTTGGAGCCCCGCCGCCCAGGATCCTGAGAAGGGGGAAAGACTGGGAGGA
GCTCGGAGAGGAGATGTCCCAAACAGGAAGGAAGAGGAACCGGAGGAAGTCCCAAGAGCC
AAAGGGCCTAGAAAGGCTCCTGTGAAGGAGAGTCCTGAAGTGCTGGTGGAACGCAACCCT
GACCCAGCTATTAGCGTGGCCCCGGCACGGGCACAGCCACCCAAAAATGCTGCCTGGGAC
CCGACCACAGGAGCACAGCCCCCGGCACCCATACCCAGCATGGATGCTCAGGCCGGCCAG
CGGCGCCACGTGTGCACGGACTGCGGCCGCCGCTTCACCTACCCCTCACTGCTGGTCAGC
CACAGGCGCATGCACTCGGGGGAGCGGCCTTTCCCCTGCCCCGAGTGTGGCATGCGCTTC
AAGAGGAAGTTCGCAGTGGAAGCGCACCAGTGGATCCACCGCTCCTGCTCCGGGGGGCGG
CGGGGCCGGAGGCCTGGGATCCGGGCTGTGCCTCGGGCCCCCGTCCGAGGTGACCGGGAC
CCGCCTGTGCTCTTCCGGCACTACCCAGACATCTTCGAGGAGTGCGGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 897
Start on reference sequence 67
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF688
Symbol Nomenclature : ZNF688
Full Nomenclature : zinc finger protein 688
GENEID : 146542
HGNC : 30489
Target GenBank: BC018997

 

Reference Sequence Alignment

  

HsCD00079552        ATGGCGCCGCCCCCAGCCCCGCTCCTGGCGCCGAGGCCCGGGGAGACCCGGCCTGGTTGC
NM_145271.3         ATGGCGCCGCCCCCAGCCCCGCTCCTGGCGCCGAGGCCCGGGGAGACCCGGCCTGGTTGC
NM_001024683.1      -------------------------ATGCCGAGGTCTCCGCTGCGCTCCAGTCTGTCAAC
                                             : **  .*.   ***  *.*. **.* ***  :.*

HsCD00079552        AGGAAGCCCGGGACTGTGAGCTTCGCGGACGTGGCCGTGTACTTCTCCCCGGAGGAGTGG
NM_145271.3         AGGAAGCCCGGGACTGTGAGCTTCGCGGACGTGGCCGTGTACTTCTCCCCGGAGGAGTGG
NM_001024683.1      AGAGGCTTCAGGACCGCGGGGTCCCTTCAAATGCCCCG-----------------CCAGT
                    **...   *.**** * *.* * *    *..** **                   . :* 

HsCD00079552        GGCTGTCTGCGGCCCGCGCAGAGGGCTCTGTACCGGGACGTGATGCAGGAGACCTACGGC
NM_145271.3         GGCTGTCTGCGGCCCGCGCAGAGGGCTCTGTACCGGGACGTGATGCAGGAGACCTACGGC
NM_001024683.1      CCCAGGCCAATCACCGCGCAGCTTCTTCCGGGATCGTCGCCAATCATCGGCCGTTGCGTA
                      *:* * ..  .********.    ** * ..  * .   .** .: *. .  *.** .

HsCD00079552        CACCTGGGCGCGCTCGGATTCCCAGGCCCCAAACCAGCCCTCATCTCTTGGATGGAACAG
NM_145271.3         CACCTGGGCGCGCTCGGATTCCCAGGCCCCAAACCAGCCCTCATCTCTTGGATGGAACAG
NM_001024683.1      TTGCGGGTACAGAGGGGATTCCCAGGCCCCAAACCAGCCCTCATCTCTTGGATGGAACAG
                     : * ** . .*.  *********************************************

HsCD00079552        GAGAGTGAGGCTTGGAGCCCCGCCGCCCAGGATCCTGAGAAGGGGGAAAGACTGGGAGGA
NM_145271.3         GAGAGTGAGGCTTGGAGCCCCGCCGCCCAGGATCCTGAGAAGGGGGAAAGACTGGGAGGA
NM_001024683.1      GAGAGTGAGGCTTGGAGCCCCGCCGCCCAGGATCCTGAGAAGGGGGAAAGACTGGGAGGA
                    ************************************************************

HsCD00079552        GCTCGGAGAGGAGATGTCCCAAACAGGAAGGAAGAGGAACCGGAGGAAGTCCCAAGAGCC
NM_145271.3         GCTCGGAGAGGAGATGTCCCAAACAGGAAGGAAGAGGAACCGGAGGAAGTCCCAAGAGCC
NM_001024683.1      GCTCGGAGAGGAGATGTCCCAAACAGGAAGGAAGAGGAACCGGAGGAAGTCCCAAGAGCC
                    ************************************************************

HsCD00079552        AAAGGGCCTAGAAAGGCTCCTGTGAAGGAGAGTCCTGAAGTGCTGGTGGAACGCAACCCT
NM_145271.3         AAAGGGCCTAGAAAGGCTCCTGTGAAGGAGAGTCCTGAAGTGCTGGTGGAACGCAACCCT
NM_001024683.1      AAAGGGCCTAGAAAGGCTCCTGTGAAGGAGAGTCCTGAAGTGCTGGTGGAACGCAACCCT
                    ************************************************************

HsCD00079552        GACCCAGCTATTAGCGTGGCCCCGGCACGGGCACAGCCACCCAAAAATGCTGCCTGGGAC
NM_145271.3         GACCCAGCTATTAGCGTGGCCCCGGCACGGGCACAGCCACCCAAAAATGCTGCCTGGGAC
NM_001024683.1      GACCCAGCTATTAGCGTGGCCCCGGCACGGGCACAGCCACCCAAAAATGCTGCCTGGGAC
                    ************************************************************

HsCD00079552        CCGACCACAGGAGCACAGCCCCCGGCACCCATACCCAGCATGGATGCTCAGGCCGGCCAG
NM_145271.3         CCGACCACAGGAGCACAGCCCCCGGCACCCATACCCAGCATGGATGCTCAGGCCGGCCAG
NM_001024683.1      CCGACCACAGGAGCACAGCCCCCGGCACCCATACCCAGCATGGATGCTCAGGCCGGCCAG
                    ************************************************************

HsCD00079552        CGGCGCCACGTGTGCACGGACTGCGGCCGCCGCTTCACCTACCCCTCACTGCTGGTCAGC
NM_145271.3         CGGCGCCACGTGTGCACGGACTGCGGCCGCCGCTTCACCTACCCCTCACTGCTGGTCAGC
NM_001024683.1      CGGCGCCACGTGTGCACGGACTGCGGCCGCCGCTTCACCTACCCCTCACTGCTGGTCAGC
                    ************************************************************

HsCD00079552        CACAGGCGCATGCACTCGGGGGAGCGGCCTTTCCCCTGCCCCGAGTGTGGCATGCGCTTC
NM_145271.3         CACAGGCGCATGCACTCGGGGGAGCGGCCTTTCCCCTGCCCCGAGTGTGGCATGCGCTTC
NM_001024683.1      CACAGGCGCATGCACTCGGGGGAGCGGCCTTTCCCCTGCCCCGAGTGTGGCATGCGCTTC
                    ************************************************************

HsCD00079552        AAGAGGAAGTTCGCAGTGGAAGCGCACCAGTGGATCCACCGCTCCTGCTCCGGGGGGCGG
NM_145271.3         AAGAGGAAGTTCGCAGTGGAAGCGCACCAGTGGATCCACCGCTCCTGCTCCGGGGGGCGG
NM_001024683.1      AAGAGGAAGTTCGCAGTGGAAGCGCACCAGTGGATCCACCGCTCCTGCTCCGGGGGGCGG
                    ************************************************************

HsCD00079552        CGGGGCCGGAGGCCTGGGATCCGGGCTGTGCCTCGGGCCCCCGTCCGAGGTGACCGGGAC
NM_145271.3         CGGGGCCGGAGGCCTGGGATCCGGGCTGTGCCTCGGGCCCCCGTCCGAGGTGACCGGGAC
NM_001024683.1      CGGGGCCGGAGGCCTGGGATCCGGGCTGTGCCTCGGGCCCCCGTCCGAGGTGACCGGGAC
                    ************************************************************

HsCD00079552        CCGCCTGTGCTCTTCCGGCACTACCCAGACATCTTCGAGGAGTGCGGCTTG
NM_145271.3         CCGCCTGTGCTCTTCCGGCACTACCCAGACATCTTCGAGGAGTGCGGCTGA
NM_001024683.1      CCGCCTGTGCTCTTCCGGCACTACCCAGACATCTTCGAGGAGTGCGGCTGA
                    ************************************************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : P0C7X2
UniProt : A8MV39
HPRD : 14070

Clone

Cloning Information : 257058
HIP Master Clone ID : 193106
Original Clone ID : FLH257058.01L

Organism


TAX_ID : 9606
Species Specific ID: 146542

Genome

Chromosome : 16
Map Location : 16p11.2
Ensembl : ENSG00000229809

Reagents

Labome : ZNF688-antibody