DNASU Plasmid Repository • 480.965.5697 | Email

RNF41 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  RNF41
Gene Name:  ring finger protein 41
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH257064.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079558

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 954nts         Open reading frame : 1 to 954
Reference Sequence Alignment

ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1182
Start on reference sequence 229
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNF41
Symbol Nomenclature : RNF41
SYNONYM : FLRF; NRDP1
Designation : E3 ubiquitin-protein ligase NRDP1|fetal liver ring finger|neuregulin receptor degradation protein-1
Full Nomenclature : ring finger protein 41
GENEID : 10193
Locus Tag : SBBI03
HGNC : 18401
Target GenBank: BC032637

 

Reference Sequence Alignment

  

HsCD00079558        ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_001242826.1      ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_005785.3         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_194359.2         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
                    ************************************************************

HsCD00079558        TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_001242826.1      TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_005785.3         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_194359.2         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
                    ************************************************************

HsCD00079558        GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_001242826.1      GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_005785.3         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_194359.2         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
                    ************************************************************

HsCD00079558        ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_001242826.1      ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_005785.3         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_194359.2         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
                    ************************************************************

HsCD00079558        ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_001242826.1      ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_005785.3         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_194359.2         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
                    ************************************************************

HsCD00079558        TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_001242826.1      TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_005785.3         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_194359.2         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
                    ************************************************************

HsCD00079558        GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_001242826.1      GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_005785.3         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_194359.2         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
                    ************************************************************

HsCD00079558        GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_001242826.1      GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_005785.3         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_194359.2         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
                    ************************************************************

HsCD00079558        CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_001242826.1      CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_005785.3         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_194359.2         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
                    ************************************************************

HsCD00079558        CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_001242826.1      CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_005785.3         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_194359.2         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
                    ************************************************************

HsCD00079558        GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_001242826.1      GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_005785.3         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_194359.2         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
                    ************************************************************

HsCD00079558        CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_001242826.1      CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_005785.3         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_194359.2         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
                    ************************************************************

HsCD00079558        TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_001242826.1      TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_005785.3         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_194359.2         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
                    ************************************************************

HsCD00079558        ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_001242826.1      ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_005785.3         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_194359.2         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
                    ************************************************************

HsCD00079558        CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_001242826.1      CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_005785.3         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_194359.2         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
                    ************************************************************

HsCD00079558        GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATTG
NM_001242826.1      GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_005785.3         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_194359.2         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
                    ****************************************************:.

Pathways

NCI : ErbB2/ErbB3 signaling events
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Downregulation of ERBB2:ERBB3 signaling
Reactome : Immune System
Reactome : Signal Transduction
Reactome : Signaling by ERBB2
WikiPathway : Regulation of toll-like receptor signaling pathway

Protein

SMART domain : Ubox : Modified RING finger domain
SMART domain : RING : Ring finger
UniProt : A6NFW0
UniProt : Q9H4P4
HPRD : 11520

Clone

Cloning Information : 257064
HIP Master Clone ID : 193112
Original Clone ID : FLH257064.01L

Organism


TAX_ID : 9606
Species Specific ID: 10193

Genome

Chromosome : 12
Map Location : 12q13.13

Reagents

Labome : RNF41-antibody