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ZNF528 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF528
Gene Name:  zinc finger protein 528
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH257082.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079573

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1887nts         Open reading frame : 1 to 1887
Reference Sequence Alignment

ATGGCCCTTACTCAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAA
GAGTGGAAATGCCTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAAT
TATAGGAACCTGGTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTA
GAGCAAAAGAGAGATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGAC
GGCAGGGAGTGCATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCA
GGAAACAAGCCTTGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTA
CAGCTATTTCAAGCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGT
GACAATTCCTCAGTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTA
AATAAATATAGAAATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACAC
ATTAGGGAAAAAGCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGC
CTTACTAACCAAGTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAG
GTCTTTAGTTGCAGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCT
TACAAATGTCATGAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAA
AGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGC
AGTTCAAAGCTTGCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCAT
GAATGTGACAAGGTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACT
GGAGAGAAACCTTACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTA
GCAGAACATCAAACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAA
GCATTTTCAGTGCGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCT
TACAAATGTAAAGAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAG
AGAATTCATACTAGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAG
AAATCAGACCTTATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAAT
AAATGTGGCACAGCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGT
GGAGAGAAACCTTATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTA
ACCAGTCATCATAGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAG
GTCTTCAGTCGCAGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCT
TACAAATGTAATCAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAA
ATAATTCATACTGGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGG
TGTTCAGGCCTTACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAA
GAATGTGGCAAGATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATT
GGAGAGAAACCTTACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTT
GCACAGCATCAGAGAGTTCATTCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1941
Start on reference sequence 55
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF528
Symbol Nomenclature : ZNF528
Full Nomenclature : zinc finger protein 528
GENEID : 84436
HGNC : 29384
MIM : 615580
Vega : OTTHUMG00000156494
Target GenBank: BC111950

 

Reference Sequence Alignment

  

HsCD00079573        ------------------------------------------------------------
XM_005259316.1      ATGAAGAATGAGAGACCCATTAACAGAAGGCAAAGTAGAAGGATTCACTTCCAAAGAGAC
NM_032423.2         ------------------------------------------------------------
                                                                                

HsCD00079573        ------------------------------------------------ATGGCCCTTACT
XM_005259316.1      ATATTATGCAAGGAAGCAACTTGGAAGAGGAAAGAAAAGAAGTCAGGAATGGCCCTTACT
NM_032423.2         ------------------------------------------------ATGGCCCTTACT
                                                                    ************

HsCD00079573        CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
XM_005259316.1      CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
NM_032423.2         CAGGGACCCTTGAAATTCATGGATGTGGCCATAGAGTTCTCTCAGGAAGAGTGGAAATGC
                    ************************************************************

HsCD00079573        CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
XM_005259316.1      CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
NM_032423.2         CTGGACCCTGCGCAGAGGACTTTATACAGGGACGTGATGTTGGAGAATTATAGGAACCTG
                    ************************************************************

HsCD00079573        GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
XM_005259316.1      GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
NM_032423.2         GTCTCCCTGGGAATCTGTCTTCCTGACCTGAGTGTTACCTCCATGTTAGAGCAAAAGAGA
                    ************************************************************

HsCD00079573        GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
XM_005259316.1      GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
NM_032423.2         GATCCCTGGACTCTGCAGAGTGAAGAGAAAATAGCAAACGATCCAGACGGCAGGGAGTGC
                    ************************************************************

HsCD00079573        ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
XM_005259316.1      ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
NM_032423.2         ATCAAAGGTGTGAACACAGAGAGGAGCTCTAAATTGGGAAGTAATGCAGGAAACAAGCCT
                    ************************************************************

HsCD00079573        TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
XM_005259316.1      TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
NM_032423.2         TGTAAAAATCAACTTGGATTCACTTTTCAGTTACATCTGAGTGATCTACAGCTATTTCAA
                    ************************************************************

HsCD00079573        GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
XM_005259316.1      GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
NM_032423.2         GCTGAAAGGAAAATTTCTGGGTGTAAGCATTTTGAAAAACCCGTCAGTGACAATTCCTCA
                    ************************************************************

HsCD00079573        GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
XM_005259316.1      GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
NM_032423.2         GTTTCACCGCTTGAAAAAATTTCTTCCAGTGTCAAATCCCACCTTTTAAATAAATATAGA
                    ************************************************************

HsCD00079573        AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
XM_005259316.1      AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
NM_032423.2         AATAATTTTGATCATGCTCCATTACTTCCACAAGAACAGAAAGCACACATTAGGGAAAAA
                    ************************************************************

HsCD00079573        GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
XM_005259316.1      GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
NM_032423.2         GCTTATAAATGTAATGAGCACGGCCAAGTCTTTAGAGCATCTGCAAGCCTTACTAACCAA
                    ************************************************************

HsCD00079573        GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
XM_005259316.1      GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
NM_032423.2         GTAATCCATAACGCAGATAATCCTTACAAATGCAGTGAATGTGGCAAGGTCTTTAGTTGC
                    ************************************************************

HsCD00079573        AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
XM_005259316.1      AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
NM_032423.2         AGTTCAAAGCTTGTGATACATCGAAGAATGCATACTGGAGAGAAGCCTTACAAATGTCAT
                    ************************************************************

HsCD00079573        GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
XM_005259316.1      GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
NM_032423.2         GAATGTGGCAAGCTCTTCAGTAGCAATTCAAACCTTTCACAACATCAAAGAATTCATACT
                    ************************************************************

HsCD00079573        GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
XM_005259316.1      GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
NM_032423.2         GGAGAGAAGCCTTACAAATGTCATGAATGTGACAAGGTCTTTCGAAGCAGTTCAAAGCTT
                    ************************************************************

HsCD00079573        GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
XM_005259316.1      GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
NM_032423.2         GCACAACATCAAAGAATTCATACTGGAGAGAAGCCTTACAAATGTCATGAATGTGACAAG
                    ************************************************************

HsCD00079573        GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
XM_005259316.1      GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
NM_032423.2         GTCTTCAATCAAATTGCACACCTTGTACGACATCAAAAAATTCATACTGGAGAGAAACCT
                    ************************************************************

HsCD00079573        TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
XM_005259316.1      TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
NM_032423.2         TACAGTTGTAATAAATGTGGCAAGGTCTTTAGTCGCCATTCATATCTAGCAGAACATCAA
                    ************************************************************

HsCD00079573        ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
XM_005259316.1      ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
NM_032423.2         ACGGTTCATACTGGTGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCATTTTCAGTG
                    ************************************************************

HsCD00079573        CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
XM_005259316.1      CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
NM_032423.2         CGTTCCAGCCTCATAACCCATCAGTTAATTCACACTGGAAGGAAACCTTACAAATGTAAA
                    ************************************************************

HsCD00079573        GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
XM_005259316.1      GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
NM_032423.2         GAATGTGACAAGGTCTTTGGGCGCAAGTGTTTCCTGACCTCTCATCAGAGAATTCATACT
                    ************************************************************

HsCD00079573        AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
XM_005259316.1      AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
NM_032423.2         AGAGAGAGACCTTATGGATGCAGTCAGTGTGGCAAGATCTTTAGTCAGAAATCAGACCTT
                    ************************************************************

HsCD00079573        ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
XM_005259316.1      ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
NM_032423.2         ATACGACATCGAAAAACTCATACTGATGAGAAGCCTTACAAATGTAATAAATGTGGCACA
                    ************************************************************

HsCD00079573        GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
XM_005259316.1      GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
NM_032423.2         GCGTTTAGAGAGTTTTCAGACCTTACTGCCCATTTTCTAATCCATAGTGGAGAGAAACCT
                    ************************************************************

HsCD00079573        TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
XM_005259316.1      TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
NM_032423.2         TATGAATGTAAAGAATGTGGCAAAGTCTTCAGGTACAAGTCTTCTCTAACCAGTCATCAT
                    ************************************************************

HsCD00079573        AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
XM_005259316.1      AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
NM_032423.2         AGAATTCATACTGGAGAGAAGCCTTACAAATGTAACAGATGTGGCAAGGTCTTCAGTCGC
                    ************************************************************

HsCD00079573        AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
XM_005259316.1      AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
NM_032423.2         AGTTCAAACCTGGTATGCCATCAGAAAATTCATACAGGAGAAAAGCCTTACAAATGTAAT
                    ************************************************************

HsCD00079573        CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
XM_005259316.1      CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
NM_032423.2         CAATGTGGCAAGGTCTTTAATCAAGCATCATACCTTACAAGACATCAAATAATTCATACT
                    ************************************************************

HsCD00079573        GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
XM_005259316.1      GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
NM_032423.2         GGAGAGAGGCCTTACAGATGTAGTAAATGTGGCAAAGCATTTCGAGGGTGTTCAGGCCTT
                    ************************************************************

HsCD00079573        ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
XM_005259316.1      ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
NM_032423.2         ACTGCCCATCTTGCAATCCATACTGAAAAGAAATCTCATGAGTGTAAAGAATGTGGCAAG
                    ************************************************************

HsCD00079573        ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
XM_005259316.1      ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
NM_032423.2         ATCTTCACTCAGAAGTCTTCCCTCACCAATCACCATAGAATTCACATTGGAGAGAAACCT
                    ************************************************************

HsCD00079573        TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
XM_005259316.1      TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
NM_032423.2         TACAAATGCACCCTGTGCAGTAAGGTCTTCAGTCACAATTCTGACCTTGCACAGCATCAG
                    ************************************************************

HsCD00079573        AGAGTTCATTCATTG
XM_005259316.1      AGAGTTCATTCATGA
NM_032423.2         AGAGTTCATTCATGA
                    ************* .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q3MIS6
HPRD : 15820

Clone

Cloning Information : 257082
HIP Master Clone ID : 193130
Original Clone ID : FLH257082.01L

Organism


TAX_ID : 9606
Species Specific ID: 84436

Genome

Chromosome : 19
Map Location : 19q13
Ensembl : ENSG00000167555

Reagents

Labome : ZNF528-antibody