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ZNF561 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF561
Gene Name:  zinc finger protein 561
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH257091.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079582

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1254nts         Open reading frame : 1 to 1254
Reference Sequence Alignment

ATGCTGGAGAACTACATGAACCTGGCCTCTGTGGAATGGGAAATACAACCTAGAACCAAA
CGGTCATCACTTCAGCAGGGTTTTTTGAAGAATCAAATATTCAGTGGGATACAAATGACA
AGAGGCTACAGTGGATGGAAACTCTGTGACTGTAAGAATTGTGGAGAGGTCTTCAGGGAA
CAGTTTTGCCTTAAGACACACATGAGAGTTCAGAATGGAGGGAATACTTCTGAGGGTAAT
TGTTATGGAAAAGACACCCTCAGTGTGCACAAGGAAGCCTCTACTGGACAGGAACTTTCC
AAATTTAATCCATGTGGAAAAGTCTTTACTCTAACTCCAGGTCTTGCTGTACATCTTGAA
GTTCTCAATGCAAGACAACCCTACAAATGTAAGGAATGTGGAAAAGGCTTTAAGTATTTT
GCAAGCCTTGATAATCATATGGGAATCCACACTGATGAGAAACTCTGTGAATTTCAGGAA
TATGGGAGAGCTGTCACAGCTTCTTCACACCTAAAGCAGTGTGTAGCAGTTCATACAGGA
AAGAAATCCAAAAAGACTAAGAAATGTGGGAAATCCTTCACTAATTTTTCTCAACTTTAT
GCACCTGTGAAAACTCATAAAGGAGAGAAGTCCTTTGAATGTAAAGAATGTGGAAGATCC
TTTAGAAATTCCTCATGCCTTAATGATCACATTCAAATTCACACTGGAATAAAACCACAC
AAGTGTACTTACTGTGGGAAAGCCTTCACTAGATCAACTCAACTTACTGAACATGTAAGA
ACTCACACTGGAATAAAACCCTATGAATGTAAGGAATGTGGCCAAGCCTTTGCTCAGTAC
TCGGGCCTTTCTATACACATACGAAGTCACAGTGGAAAGAAACCCTATCAGTGTAAGGAA
TGTGGGAAAGCCTTCACTACATCCACAAGTCTTATTCAGCATACAAGAATTCACACAGGA
GAGAAGCCTTATGAATGTGTTGAATGTGGGAAGACCTTCATTACTTCTTCCCGTCGTAGT
AAACATTTGAAAACTCATAGTGGAGAAAAGCCCTTTGTATGCAAGATATGTGGGAAAGCA
TTTCTATATTCCTCACGCCTTAATGTTCACCTGCGAACTCATACCGGAGAGAAACCCTTC
GTATGTAAAGAATGTGGGAAAGCATTTGCTGTTTCCTCACGCCTAAGTAGACATGAAAGA
ATTCACACTGGGGAGAAACCCTATGAATGCAAGGATATGAGTGTTACCATTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1658
Start on reference sequence 405
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF561
Symbol Nomenclature : ZNF561
Full Nomenclature : zinc finger protein 561
GENEID : 93134
HGNC : 28684
Vega : OTTHUMG00000157054
Target GenBank: BC032668

 

Reference Sequence Alignment

  

HsCD00079582        ------------------------------------------------------------
NM_152289.2         ATGGCAGCCATTTATTTGTCCCGTGGGTTTTTTTCCAGGGAACCAATCTGCCCTTTTGAA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      ATGGCAGCCATTTATTTGTCCCGTGGGTTTTTTTCCAGGGAACCAATCTGCCCTTTTGAA
                                                                                

HsCD00079582        ------------------------------------------------------------
NM_152289.2         GAAAAGACAAAGGTAGAAAGGATGGTGGAGGACTACCTGGCAAGTGGTTATCAGGATTCA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GAAAAGACAAAGGTAGAAAGGATGGTGGAGGACTACCTGGCAAGTGGTTATCAGGATTCA
                                                                                

HsCD00079582        ------------------------------------------------------------
NM_152289.2         GTGACGTTTGATGATGTGGCTGTGGACTTCACCCCAGAGGAGTGGGCTTTACTGGACACA
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GTGACGTTTGATGATGTGGCTGTGGACTTCACCCCAGAGGAGTGGGCTTTACTGGACACA
                                                                                

HsCD00079582        ---------------------------ATGCTGGAGAACTACATGAACCTGGCCTCTGTG
NM_152289.2         ACTGAGAAATACCTCTACAGAGATGTGATGCTGGAGAACTACATGAACCTGGCCTCTGTG
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      ACTGAGAAATACCTCTACAGAGATGTGATGCTGGAGAACTACATGAACCTGGCCTCTGTG
                                                                                

HsCD00079582        GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
NM_152289.2         GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
XM_005260151.1      ------------------------------------------------------------
XM_005260150.1      GAATGGGAAATACAACCTAGAACCAAACGGTCATCACTTCAGCAGGGTTTTTTGAAGAAT
                                                                                

HsCD00079582        CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
NM_152289.2         CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
XM_005260151.1      ---------------------ATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
XM_005260150.1      CAAATATTCAGTGGGATACAAATGACAAGAGGCTACAGTGGATGGAAACTCTGTGACTGT
                                         ***************************************

HsCD00079582        AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
NM_152289.2         AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
XM_005260151.1      AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
XM_005260150.1      AAGAATTGTGGAGAGGTCTTCAGGGAACAGTTTTGCCTTAAGACACACATGAGAGTTCAG
                    ************************************************************

HsCD00079582        AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
NM_152289.2         AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
XM_005260151.1      AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
XM_005260150.1      AATGGAGGGAATACTTCTGAGGGTAATTGTTATGGAAAAGACACCCTCAGTGTGCACAAG
                    ************************************************************

HsCD00079582        GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
NM_152289.2         GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
XM_005260151.1      GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
XM_005260150.1      GAAGCCTCTACTGGACAGGAACTTTCCAAATTTAATCCATGTGGAAAAGTCTTTACTCTA
                    ************************************************************

HsCD00079582        ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
NM_152289.2         ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
XM_005260151.1      ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
XM_005260150.1      ACTCCAGGTCTTGCTGTACATCTTGAAGTTCTCAATGCAAGACAACCCTACAAATGTAAG
                    ************************************************************

HsCD00079582        GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
NM_152289.2         GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
XM_005260151.1      GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
XM_005260150.1      GAATGTGGAAAAGGCTTTAAGTATTTTGCAAGCCTTGATAATCATATGGGAATCCACACT
                    ************************************************************

HsCD00079582        GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
NM_152289.2         GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
XM_005260151.1      GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
XM_005260150.1      GATGAGAAACTCTGTGAATTTCAGGAATATGGGAGAGCTGTCACAGCTTCTTCACACCTA
                    ************************************************************

HsCD00079582        AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
NM_152289.2         AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
XM_005260151.1      AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
XM_005260150.1      AAGCAGTGTGTAGCAGTTCATACAGGAAAGAAATCCAAAAAGACTAAGAAATGTGGGAAA
                    ************************************************************

HsCD00079582        TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
NM_152289.2         TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
XM_005260151.1      TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
XM_005260150.1      TCCTTCACTAATTTTTCTCAACTTTATGCACCTGTGAAAACTCATAAAGGAGAGAAGTCC
                    ************************************************************

HsCD00079582        TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
NM_152289.2         TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
XM_005260151.1      TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
XM_005260150.1      TTTGAATGTAAAGAATGTGGAAGATCCTTTAGAAATTCCTCATGCCTTAATGATCACATT
                    ************************************************************

HsCD00079582        CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
NM_152289.2         CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
XM_005260151.1      CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
XM_005260150.1      CAAATTCACACTGGAATAAAACCACACAAGTGTACTTACTGTGGGAAAGCCTTCACTAGA
                    ************************************************************

HsCD00079582        TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
NM_152289.2         TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
XM_005260151.1      TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
XM_005260150.1      TCAACTCAACTTACTGAACATGTAAGAACTCACACTGGAATAAAACCCTATGAATGTAAG
                    ************************************************************

HsCD00079582        GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
NM_152289.2         GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
XM_005260151.1      GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
XM_005260150.1      GAATGTGGCCAAGCCTTTGCTCAGTACTCGGGCCTTTCTATACACATACGAAGTCACAGT
                    ************************************************************

HsCD00079582        GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
NM_152289.2         GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
XM_005260151.1      GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
XM_005260150.1      GGAAAGAAACCCTATCAGTGTAAGGAATGTGGGAAAGCCTTCACTACATCCACAAGTCTT
                    ************************************************************

HsCD00079582        ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
NM_152289.2         ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
XM_005260151.1      ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
XM_005260150.1      ATTCAGCATACAAGAATTCACACAGGAGAGAAGCCTTATGAATGTGTTGAATGTGGGAAG
                    ************************************************************

HsCD00079582        ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
NM_152289.2         ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
XM_005260151.1      ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
XM_005260150.1      ACCTTCATTACTTCTTCCCGTCGTAGTAAACATTTGAAAACTCATAGTGGAGAAAAGCCC
                    ************************************************************

HsCD00079582        TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
NM_152289.2         TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
XM_005260151.1      TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
XM_005260150.1      TTTGTATGCAAGATATGTGGGAAAGCATTTCTATATTCCTCACGCCTTAATGTTCACCTG
                    ************************************************************

HsCD00079582        CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
NM_152289.2         CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
XM_005260151.1      CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
XM_005260150.1      CGAACTCATACCGGAGAGAAACCCTTCGTATGTAAAGAATGTGGGAAAGCATTTGCTGTT
                    ************************************************************

HsCD00079582        TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
NM_152289.2         TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
XM_005260151.1      TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
XM_005260150.1      TCCTCACGCCTAAGTAGACATGAAAGAATTCACACTGGGGAGAAACCCTATGAATGCAAG
                    ************************************************************

HsCD00079582        GATATGAGTGTTACCATTTTG
NM_152289.2         GATATGAGTGTTACCATTTAG
XM_005260151.1      GATATGAGTGTTACCATTTAG
XM_005260150.1      GATATGAGTGTTACCATTTAG
                    *******************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8N587
HPRD : 15843

Clone

Cloning Information : 257091
HIP Master Clone ID : 193139
Original Clone ID : FLH257091.01L

Organism


TAX_ID : 9606
Species Specific ID: 93134

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000171469

Reagents

Labome : ZNF561-antibody