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ZNF582 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF582
Gene Name:  zinc finger protein 582
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH257139.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00079623

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1554nts         Open reading frame : 1 to 1554
Reference Sequence Alignment

ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1635
Start on reference sequence 82
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF582
Symbol Nomenclature : ZNF582
Full Nomenclature : zinc finger protein 582
GENEID : 147948
HGNC : 26421
MIM : 615600
Vega : OTTHUMG00000181939
Target GenBank: BC101009

 

Reference Sequence Alignment

  

HsCD00079623      ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
NM_144690.1       ATGTCCCTTGGGTCAGAATTGTTCAGGGATGTGGCCATAGTCTTCTCCCAAGAAGAATGG
                  ************************************************************

HsCD00079623      CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
NM_144690.1       CAGTGGTTGGCACCTGCTCAGAGGGATTTGTACAGAGACGTGATGTTGGAGACCTACAGC
                  ************************************************************

HsCD00079623      AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
NM_144690.1       AACCTGGTCTCACTGGGTCTTGCCGTTTCCAAACCTGATGTGATCTCCTTCCTAGAGCAA
                  ************************************************************

HsCD00079623      GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
NM_144690.1       GGCAAAGAGCCCTGGATGGTGGAGAGAGTGGTGTCTGGAGGCCTGTGTCCAGTATTGGAG
                  ************************************************************

HsCD00079623      TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
NM_144690.1       TCCAGATATGATACCAAGGAATTATTTCCAAAGCAGCATGTTTATGAAGTCGAATCACCC
                  ************************************************************

HsCD00079623      CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
NM_144690.1       CAATGGGAGATAATGGAAAGCCTTACAAGTTATGGTCTTGAGTGTTCAAGTTTCCAAGAT
                  ************************************************************

HsCD00079623      GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
NM_144690.1       GATTGGGAATGCAGAAACCAGTTTGACAGACAACAGGGAAATCCAGACAGACATTTCCAT
                  ************************************************************

HsCD00079623      CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
NM_144690.1       CAGATGATCATCAGACATGAAGAAATGCCCACTTTTGACCAGCATGCATCCCTTACTTTT
                  ************************************************************

HsCD00079623      TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
NM_144690.1       TATCAGAAAATTCATACTAGAGAAAAACCTTTTGGGTATAATAAATGTAGAAAAGACTTC
                  ************************************************************

HsCD00079623      TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
NM_144690.1       TGGCAAAAGGAACTCCTTATTAATCATCAAGGAATTTATACTAATGAGAAACCCTATAAA
                  ************************************************************

HsCD00079623      TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
NM_144690.1       TGTAAGGAATGTGGGAAGGCCTTTAAATATGGCTCACGACTAATTCAACATGAGAATATT
                  ************************************************************

HsCD00079623      CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
NM_144690.1       CATTCTGGTAAGAAACCATATGAATGTAAGGAATGTGGAAAGGCCTTCAATTCTGGTTCA
                  ************************************************************

HsCD00079623      AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
NM_144690.1       AATTTTATACAACATCAGAGAGTTCATACTGGTGAGAAACCGTATGAATGTAAAGATTGT
                  ************************************************************

HsCD00079623      GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
NM_144690.1       GAGAAGGCCTTTAGTCGAAGCTCACAGTTGATTGAACATCAGCGAACTCACACAGGCGAG
                  ************************************************************

HsCD00079623      AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
NM_144690.1       AAACCCTATCAGTGTAAGGAATGTGGCAAGGCCTTCAATCGGATCTCACATCTTAAAGTA
                  ************************************************************

HsCD00079623      CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
NM_144690.1       CATTATAGAATTCATACTGGTGAAAAACCCTATGCATGCAAGGAATGTGGGAAAACCTTT
                  ************************************************************

HsCD00079623      AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
NM_144690.1       AGTCATCGTTCTCAGTTGATTCAACATCAGACTGTTCATACTGGCAGGAAACTCTATGAA
                  ************************************************************

HsCD00079623      TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
NM_144690.1       TGTAAGGAATGTGGGAAGGCTTTTAATCAAGGCTCAACTCTTATACGACATCAGAGAATT
                  ************************************************************

HsCD00079623      CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
NM_144690.1       CATACCGGTGAGAAACCCTATGAGTGCAAAGTATGTGGAAAGGCCTTTAGAGTGAGCTCA
                  ************************************************************

HsCD00079623      CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
NM_144690.1       CAACTCAAGCAACATCAGAGAATTCACACTGGAGAGAAACCCTACCAATGTAAGGTATGT
                  ************************************************************

HsCD00079623      GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
NM_144690.1       GGTAGGGCCTTCAAACGGGTCTCACATCTTACTGTACATTACAGAATTCATACAGGTGAA
                  ************************************************************

HsCD00079623      AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
NM_144690.1       AAGCCATATGAATGTAAGGAATGTGGGAAGGCTTTTAGTCATTGCTCACAATTGATTCAT
                  ************************************************************

HsCD00079623      CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
NM_144690.1       CATCAGGTTATTCACACTGAGAAAAAGCCCTATGAATATAAGGAATGTGAGAAGACCTTG
                  ************************************************************

HsCD00079623      AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
NM_144690.1       AGTCATGATTCAACTACCGTTCAACCTCAGAGAATGCATAATAGAGAAACACATGTGAAT
                  ************************************************************

HsCD00079623      ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
NM_144690.1       ATAATAAATGTAGAAAAGCCTTCCATCAGCAGTTACCCCTTACTAATCATCAGAGAATTT
                  ************************************************************

HsCD00079623      ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTTG
NM_144690.1       ATGTTGGCAAGCAACCATATGAATGGAAGTAATGGGGAAAGCCCATTAGCCTAG
                  ****************************************************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
SMART domain : KRAB : krueppel associated box
UniProt : Q96NG8
HPRD : 15859

Clone

Cloning Information : 257139
HIP Master Clone ID : 193187
Original Clone ID : FLH257139.01L

Organism


TAX_ID : 9606
Species Specific ID: 147948

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000018869

Reagents

Labome : ZNF582-antibody