DNASU Plasmid Repository • 480.965.5697 | Email

CAPN12 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CAPN12
Gene Name:  calpain 12
Sequence             
Original Clone ID: FLH265029.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00080408

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2157nts         Open reading frame : 1 to 2157
Reference Sequence Alignment

ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2469
Start on reference sequence 310
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN12
Symbol Nomenclature : CAPN12
Designation : CANP 12|calcium-activated neutral proteinase 12|calpain-12
Full Nomenclature : calpain 12
GENEID : 147968
GI : 147897670
GenBank Accession : BC140397
HGNC : 13249
MIM : 608839
Vega : OTTHUMG00000182525
Target GenBank: NM_144691

 

Reference Sequence Alignment

  

HsCD00080408      ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
NM_144691.3       ATGGCATCCAGCAGTGGGAGGGTCACCATCCAGCTCGTGGATGAGGAGGCTGGGGTCGGA
                  ************************************************************

HsCD00080408      GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
NM_144691.3       GCCGGGCGCCTGCAGCTTTTTCGGGGCCAGAGCTATGAGGCAATTCGGGCAGCCTGCCTG
                  ************************************************************

HsCD00080408      GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
NM_144691.3       GATTCGGGGATCCTGTTCCGCGACCCTTACTTCCCTGCTGGCCCTGATGCCCTTGGCTAT
                  ************************************************************

HsCD00080408      GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
NM_144691.3       GACCAGCTGGGGCCGGACTCGGAGAAGGCCAAAGGCGTGAAATGGATGAGGCCCCATGAG
                  ************************************************************

HsCD00080408      TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
NM_144691.3       TTCTGTGCTGAGCCGAAGTTCATCTGTGAAGACATGAGCCGCACAGACGTGTGTCAGGGG
                  ************************************************************

HsCD00080408      AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
NM_144691.3       AGCCTGGGTAACTGCTGGTTCCTTGCAGCCGCCGCCTCCCTTACTCTGTATCCCCGGCTC
                  ************************************************************

HsCD00080408      CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
NM_144691.3       CTGCGCCGGGTGGTCCCTCCTGGACAGGATTTCCAGCATGGCTACGCAGGCGTCTTCCAC
                  ************************************************************

HsCD00080408      TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
NM_144691.3       TTCCAGCTCTGGCAGTTTGGCCGCTGGATGGACGTCGTGGTGGATGACAGGCTGCCCGTG
                  ************************************************************

HsCD00080408      CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
NM_144691.3       CGTGAGGGGAAGCTGATGTTCGTGCGCTCGGAACAGCGGAATGAGTTCTGGGCCCCACTC
                  ************************************************************

HsCD00080408      CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
NM_144691.3       CTGGAGAAGGCCTACGCCAAGCTCCACGGCTCCTATGAGGTGATGCGGGGCGGCCACATG
                  ************************************************************

HsCD00080408      AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
NM_144691.3       AATGAGGCTTTTGTGGATTTCACAGGCGGCGTGGGCGAGGTGCTCTATCTGAGACAAAAC
                  ************************************************************

HsCD00080408      AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
NM_144691.3       AGCATGGGGCTGTTCTCTGCCCTGCGCCATGCCCTGGCCAAGGAGTCCCTCGTGGGCGCC
                  ************************************************************

HsCD00080408      ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
NM_144691.3       ACTGCCCTGAGTGATCGGGGTGAGTACCGCACAGAAGAGGGCCTGGTAAAGGGACACGCG
                  ************************************************************

HsCD00080408      TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
NM_144691.3       TATTCCATCACGGGCACACACAAGGTGTTCCTGGGCTTCACCAAGGTGCGGCTGCTGCGG
                  ************************************************************

HsCD00080408      CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
NM_144691.3       CTGCGGAACCCATGGGGCTGCGTGGAGTGGACGGGGGCCTGGAGCGACAGCTGCCCACGC
                  ************************************************************

HsCD00080408      TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
NM_144691.3       TGGGACACACTCCCCACCGAGTGCCGCGATGCCCTGCTGGTGAAAAAGGAGGATGGCGAG
                  ************************************************************

HsCD00080408      TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
NM_144691.3       TTCTGGATGGAGCTGCGGGACTTCCTCCTCCATTTCGACACCGTGCAGATCTGCTCGCTG
                  ************************************************************

HsCD00080408      AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
NM_144691.3       AGCCCGGAGGTGCTGGGCCCCAGCCCGGAGGGGGGCGGCTGGCACGTCCACACCTTCCAA
                  ************************************************************

HsCD00080408      GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
NM_144691.3       GGCCGCTGGGTGCGTGGCTTCAACTCCGGCGGGAGCCAGCCTAATGCTGAAACCTTCTGG
                  ************************************************************

HsCD00080408      ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
NM_144691.3       ACCAATCCTCAGTTCCGTTTAACGCTGCTGGAGCCTGATGAGGAGGATGACGAGGATGAG
                  ************************************************************

HsCD00080408      GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
NM_144691.3       GAAGGGCCCTGGGGGGGCTGGGGGGCTGCAGGGGCACGGGGCCCAGCGCGGGGGGGCCGC
                  ************************************************************

HsCD00080408      ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
NM_144691.3       ACGCCCAAGTGCACGGTCCTTCTGTCCCTCATCCAGCGCAACCGGCGGCGCCTGAGAGCC
                  ************************************************************

HsCD00080408      AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
NM_144691.3       AAGGGCCTCACTTACCTCACCGTTGGCTTCCACGTGTTCCAGATTCCAGAGGAGCTGCTG
                  ************************************************************

HsCD00080408      GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
NM_144691.3       GGCCTCTGGGATTCCCCGCGCAGCCATGCGCTCCTGCCCCGGCTGCTGCGCGCCGACCGC
                  ************************************************************

HsCD00080408      TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
NM_144691.3       TCGCCCCTCAGCGCCCGCCGCGACGTGACCCGCCGCTGCTGCCTGCGTCCAGGCCACTAC
                  ************************************************************

HsCD00080408      CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
NM_144691.3       CTGGTGGTGCCGAGCACCGCCCACGCCGGCGACGAGGCTGACTTCACTCTGCGTGTCTTC
                  ************************************************************

HsCD00080408      TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
NM_144691.3       TCCGAGCGCCGCCACACGGCCGTGGAGATCGACGACGTGATCAGCGCAGACCTGCAGTCT
                  ************************************************************

HsCD00080408      CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
NM_144691.3       CTCCAGGGCCCCTACCTGCCCCTGGAGCTGGGGTTGGAGCAGCTGTTTCAGGAGCTGGCT
                  ************************************************************

HsCD00080408      GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
NM_144691.3       GGAGAGGAGGAAGAACTCAATGCCTCTCAGCTCCAGGCCTTACTAAGCATTGCCCTGGAG
                  ************************************************************

HsCD00080408      CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
NM_144691.3       CCTGCCAGGGCCCATACCTCCACCCCCAGAGAGATCGGGCTCAGGACCTGTGAGCAGCTG
                  ************************************************************

HsCD00080408      CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
NM_144691.3       CTGCAGTGTTTCGGGCATGGGCAAAGCCTGGCCTTACACCACTTCCAGCAGCTCTGGGGC
                  ************************************************************

HsCD00080408      TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
NM_144691.3       TACCTCCTGGAGTGGCAGGCCATATTTAACAAGTTCGATGAGGACACCTCTGGAACCATG
                  ************************************************************

HsCD00080408      AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
NM_144691.3       AACTCCTACGAGCTGAGGCTGGCACTGAATGCAGCAGGCTTCCACCTGAACAACCAGCTG
                  ************************************************************

HsCD00080408      ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
NM_144691.3       ACCCAGACCCTCACCAGCCGCTACCGGGATAGCCGTCTGCGTGTGGACTTCGAGCGGTTC
                  ************************************************************

HsCD00080408      GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
NM_144691.3       GTGTCCTGTGTGGCCCACCTCACCTGCATCTTCTGCCACTGCAGCCAGCACCTGGATGGG
                  ************************************************************

HsCD00080408      GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCC---
NM_144691.3       GGTGAGGGGGTCATCTGCCTGACCCACAGACAGTGGATGGAGGTGGCCACCTTCTCCTAG
                  *********************************************************   

Pathways

Panther : Huntington disease

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
UniProt : Q6ZSI9
HPRD : 07007

Clone

Cloning Information : 265029
HIP Master Clone ID : 197127
IMAGE ID : 100014486
Original Clone ID : FLH265029.01L

Organism


TAX_ID : 9606
Species Specific ID: 147968

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000182472

Reagents

Labome : CAPN12-antibody