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ZNF664 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF664
Gene Name:  zinc finger protein 664
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH265150.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00080529

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 783nts         Open reading frame : 1 to 783
Reference Sequence Alignment

ATGATCTACAAGTGCCCCATGTGTAGGGAATTTTTCTCTGAGAGAGCAGATCTTTTTATG
CATCAGAAAATTCACACAGCTGAGAAGCCCCATAAATGTGACAAGTGTGATAAGGGTTTC
TTTCATATATCAGAACTTCATATTCATTGGAGAGACCATACAGGAGAGAAGGTCTATAAA
TGTGATGATTGTGGTAAGGATTTTAGCACTACAACAAAACTTAATAGACATAAGAAAATC
CACACAGTGGAGAAGCCCTATAAATGTTACGAGTGTGGCAAAGCCTTCAATTGGAGCTCC
CATCTTCAAATTCATATGAGAGTTCATACAGGTGAGAAACCGTATGTCTGTAGTGAGTGT
GGAAGGGGCTTTAGTAATAGTTCAAACCTTTGCATGCATCAGAGAGTCCACACCGGAGAG
AAGCCCTTTAAATGTGAAGAGTGTGGGAAGGCCTTCAGGCACACCTCCAGCCTCTGCATG
CATCAAAGAGTCCACACAGGAGAGAAACCCTATAAATGTTATGAGTGTGGGAAGGCGTTC
AGTCAGAGTTCGAGCCTCTGCATCCACCAGAGAGTCCACACTGGAGAGAAACCCTATAGA
TGTTGTGGATGTGGGAAGGCCTTCAGTCAGAGTTCGAGCCTGTGCATCCACCAGAGAGTC
CACACAGGAGAGAAACCTTTCAAATGTGATGAGTGCGGAAAGGCCTTCAGTCAGAGTACG
AGCCTCTGCATCCACCAGAGAGTCCACACAAAGGAGAGAAACCATCTCAAAATATCAGTT
ATA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2616
Start on reference sequence 1831
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF664
Symbol Nomenclature : ZNF664
SYNONYM : ZFOC1; ZNF176
Designation : zinc finger Organ of Corti 1|zinc finger protein 176|zinc finger protein from organ of Corti
Full Nomenclature : zinc finger protein 664
GENEID : 144348
GI : 117644379
GenBank Accession : AM392806
HGNC : 25406
Vega : OTTHUMG00000168596
Target GenBank: AL834266

 

Reference Sequence Alignment

  

HsCD00080529        ATGATCTACAAGTGCCCCATGTGTAGGGAATTTTTCTCTGAGAGAGCAGATCTTTTTATG
NM_152437.2         ATGATCTACAAGTGCCCCATGTGTAGGGAATTTTTCTCTGAGAGAGCAGATCTTTTTATG
NM_001204298.1      ATGATCTACAAGTGCCCCATGTGTAGGGAATTTTTCTCTGAGAGAGCAGATCTTTTTATG
                    ************************************************************

HsCD00080529        CATCAGAAAATTCACACAGCTGAGAAGCCCCATAAATGTGACAAGTGTGATAAGGGTTTC
NM_152437.2         CATCAGAAAATTCACACAGCTGAGAAGCCCCATAAATGTGACAAGTGTGATAAGGGTTTC
NM_001204298.1      CATCAGAAAATTCACACAGCTGAGAAGCCCCATAAATGTGACAAGTGTGATAAGGGTTTC
                    ************************************************************

HsCD00080529        TTTCATATATCAGAACTTCATATTCATTGGAGAGACCATACAGGAGAGAAGGTCTATAAA
NM_152437.2         TTTCATATATCAGAACTTCATATTCATTGGAGAGACCATACAGGAGAGAAGGTCTATAAA
NM_001204298.1      TTTCATATATCAGAACTTCATATTCATTGGAGAGACCATACAGGAGAGAAGGTCTATAAA
                    ************************************************************

HsCD00080529        TGTGATGATTGTGGTAAGGATTTTAGCACTACAACAAAACTTAATAGACATAAGAAAATC
NM_152437.2         TGTGATGATTGTGGTAAGGATTTTAGCACTACAACAAAACTTAATAGACATAAGAAAATC
NM_001204298.1      TGTGATGATTGTGGTAAGGATTTTAGCACTACAACAAAACTTAATAGACATAAGAAAATC
                    ************************************************************

HsCD00080529        CACACAGTGGAGAAGCCCTATAAATGTTACGAGTGTGGCAAAGCCTTCAATTGGAGCTCC
NM_152437.2         CACACAGTGGAGAAGCCCTATAAATGTTACGAGTGTGGCAAAGCCTTCAATTGGAGCTCC
NM_001204298.1      CACACAGTGGAGAAGCCCTATAAATGTTACGAGTGTGGCAAAGCCTTCAATTGGAGCTCC
                    ************************************************************

HsCD00080529        CATCTTCAAATTCATATGAGAGTTCATACAGGTGAGAAACCGTATGTCTGTAGTGAGTGT
NM_152437.2         CATCTTCAAATTCATATGAGAGTTCATACAGGTGAGAAACCGTATGTCTGTAGTGAGTGT
NM_001204298.1      CATCTTCAAATTCATATGAGAGTTCATACAGGTGAGAAACCGTATGTCTGTAGTGAGTGT
                    ************************************************************

HsCD00080529        GGAAGGGGCTTTAGTAATAGTTCAAACCTTTGCATGCATCAGAGAGTCCACACCGGAGAG
NM_152437.2         GGAAGGGGCTTTAGTAATAGTTCAAACCTTTGCATGCATCAGAGAGTCCACACCGGAGAG
NM_001204298.1      GGAAGGGGCTTTAGTAATAGTTCAAACCTTTGCATGCATCAGAGAGTCCACACCGGAGAG
                    ************************************************************

HsCD00080529        AAGCCCTTTAAATGTGAAGAGTGTGGGAAGGCCTTCAGGCACACCTCCAGCCTCTGCATG
NM_152437.2         AAGCCCTTTAAATGTGAAGAGTGTGGGAAGGCCTTCAGGCACACCTCCAGCCTCTGCATG
NM_001204298.1      AAGCCCTTTAAATGTGAAGAGTGTGGGAAGGCCTTCAGGCACACCTCCAGCCTCTGCATG
                    ************************************************************

HsCD00080529        CATCAAAGAGTCCACACAGGAGAGAAACCCTATAAATGTTATGAGTGTGGGAAGGCGTTC
NM_152437.2         CATCAAAGAGTCCACACAGGAGAGAAACCCTATAAATGTTATGAGTGTGGGAAGGCGTTC
NM_001204298.1      CATCAAAGAGTCCACACAGGAGAGAAACCCTATAAATGTTATGAGTGTGGGAAGGCGTTC
                    ************************************************************

HsCD00080529        AGTCAGAGTTCGAGCCTCTGCATCCACCAGAGAGTCCACACTGGAGAGAAACCCTATAGA
NM_152437.2         AGTCAGAGTTCGAGCCTCTGCATCCACCAGAGAGTCCACACTGGAGAGAAACCCTATAGA
NM_001204298.1      AGTCAGAGTTCGAGCCTCTGCATCCACCAGAGAGTCCACACTGGAGAGAAACCCTATAGA
                    ************************************************************

HsCD00080529        TGTTGTGGATGTGGGAAGGCCTTCAGTCAGAGTTCGAGCCTGTGCATCCACCAGAGAGTC
NM_152437.2         TGTTGTGGATGTGGGAAGGCCTTCAGTCAGAGTTCGAGCCTGTGCATCCACCAGAGAGTC
NM_001204298.1      TGTTGTGGATGTGGGAAGGCCTTCAGTCAGAGTTCGAGCCTGTGCATCCACCAGAGAGTC
                    ************************************************************

HsCD00080529        CACACAGGAGAGAAACCTTTCAAATGTGATGAGTGCGGAAAGGCCTTCAGTCAGAGTACG
NM_152437.2         CACACAGGAGAGAAACCTTTCAAATGTGATGAGTGCGGAAAGGCCTTCAGTCAGAGTACG
NM_001204298.1      CACACAGGAGAGAAACCTTTCAAATGTGATGAGTGCGGAAAGGCCTTCAGTCAGAGTACG
                    ************************************************************

HsCD00080529        AGCCTCTGCATCCACCAGAGAGTCCACACAAAGGAGAGAAACCATCTCAAAATATCAGTT
NM_152437.2         AGCCTCTGCATCCACCAGAGAGTCCACACAAAGGAGAGAAACCATCTCAAAATATCAGTT
NM_001204298.1      AGCCTCTGCATCCACCAGAGAGTCCACACAAAGGAGAGAAACCATCTCAAAATATCAGTT
                    ************************************************************

HsCD00080529        ATA---
NM_152437.2         ATATAA
NM_001204298.1      ATATAA
                    ***   

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8N3J9
HPRD : 15723

Clone

Cloning Information : 265150
HIP Master Clone ID : 197248
IMAGE ID : 100001761
Original Clone ID : FLH265150.01L

Organism


TAX_ID : 9606
Species Specific ID: 144348

Genome

Chromosome : 12
Map Location : 12q24.31
Ensembl : ENSG00000179195

Reagents

Labome : ZNF664-antibody