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BC023181 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Plekhg5
Gene Name:  pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Sequence             
Original Clone ID: FLH265557.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00080934

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3213nts         Open reading frame : 1 to 3213

ATGGGGACAGGACCGGGCGTCTCCGGGCGCCGAGCAGCAGCCAGGCCCAGCTCTGAGCTA
CCCTCCCCAGACTCCCAGCTCCTCTGGGTCGGGGGTCATGCCCACAGCAGCGACAGCCAG
GTATGCCATCATGCCGACTGCCAGCAGCTGCACCACCGGGGGCCTCTCAACCTGTGTGAG
ACCTGTGATAGCAAGTTCCACAGCACCCTGCACTACGACGGCCATGTCCGATTTGACCTG
CCCCCGCAAGGCTCTGTCCTGGCCCGGAATGTTTCCACCCGGTCCTGTCCTCCCCGCACC
AGCCCTGCTGCAGACCTGGAGGAAGAAGAGGAAGGCTGTACCGACGGAAAAGGGGACCGG
AAGAGCGCCGGCCTAAAAATCTCTAAGAAGAAAGCAAGGAGGAGACACACAGATGACCCG
AGCAAGGAGTGCTTCACCTTGAAATTCGACCTGAACGTGGACATCGAAACAGAAATTGTG
CCAGCCATGAAGAAGAAGTCACTGGGGGAAGTACTGCTGCCTGTGTTTGAGAGGAAGGGC
ATCGCACTGGGCAAGGTAGACATCTACCTGGATCAATCCAACACACCCCTGTCCCTCACC
TTCGAGGCCTACAGGTTTGGAGGACACTACCTGCGGGTCAAAGCCAAGCCGGGGGACGAG
GGCAAGGTGGAGCAGGGAGTGAAGGATTCCAAGTCCCTCAGTCTGCCAGCCCTGAGGCCC
AGTGGGGCTGGGCCCCCTGTGTCGGAACGTGTGGACCCTCAGAGCCGCCGAGAGAGCAGT
CTGGACATCTTGGCCCCTGGCCGCCGTCGCAAGAACATGTCTGAATTCCTGGGAGAGGCA
GGCATCCCTGGACACGAGCCCCCCGCACCTTCCAGCTGTTCTCTGCCCGTTGGCAGCAGT
GGGGGTACCAGCAGTGGCATCAATGAGAGCTGGAAGAACCGGGCAGCCAGTCGCTTCAGC
GGCTTCTTCAGCTCCAGCCCGAGCACCAGTGCCTTTAGCCGGGAGGTAGACAAGATGGAA
CAGCTGGAGAGCAAGCTCCATGCCTACAGCCTTTTCGGGCTACCCCGGATGCCCAGGAGG
CTGCGCTTTGACCATGACTCCTGGGAGGAGGAAGAGGAAGATGACGAGGAGGATGAAGAG
AGTTCAGGCCTGAGGCTGGAGGACAGCTGGAGGGAGCTCACTGATGGGCATGAGAAGCTG
ACCAGGCGGCAGTGCCACCAACAGGAGGCAGTGTGGGAACTCCTCCACACAGAAGTCTCC
TACATCCGGAAACTACGTGTGATCACCAACCTGTTCCTGTGTTGTCTTCTTAACCTACAA
GAGTCGGGGCTCCTGTGTGAGGTTGAGGCTGAGCGCTTGTTCAGCAACATTCCTGAGATC
GCCAAGCTACACCGCGGCTTGTGGGGCAGCGTGATGGTGCCAGTGCTGGAGAAGGCGCGG
CGGACGCGGGCGCTGCTGCAGCCCAGCGACTTTCTCAAAGGCTTCAAGATGTTCGGCTCA
CTCTTCAAGCCATACATCCGATACTGTATGGAAGAGGAGGGCTGCATGGAGTACATGCGC
GGCCTGCTGCGTGACAACGATCTGTTCCGGGCATACGTCACGTGGGCGGAGAAGCACCAG
CAGTGCCAGCGGCTGAAGCTGAGTGACATGCTGGCCAAGCCCCACCAGCGGCTCACCAAA
TACCCACTGCTGCTCAAGTCGGTGCTAAGGAAGACAGATGATCCCCGCACCAAGGAAGCC
ATCGTCACCATGATCAGCTCGGTGGAACGCTTCATACACCACGTGAACACGTGCATGCGG
CAGCGGCAGGAGCGGCAGCGCCTGGCGGGGGTGGTGAGCCGGATCGATGCCTACGAGGTT
GTGGAGGGCAGCAACGACGAGGTGGATAAGCTCCTGAAGGAATTTCTACATCTGGACCTG
ACAGCACCCATGCCTGGAACCTCCCCTGAAGAGACCCGACAGCTGCTGCTGGAAGGGAGC
CTGAGGATGAAGGAGGGGAAAGATAGCAAGATGGACGTGTACTGCTTCCTCTTCACTGAC
CTACTCTTGGTGACCAAGGCCGTGAAGAAAGCAGAGAGGACGAAGGTCATCAGGCCACCG
CTACTGGTGGACAAGATTGTGTGCCGGGAACTTCGGGACCCTGGTTCCTTCCTCCTCATC
TACCTGAATGAATTCCACAGTGCTGTGGGGGCCTACACTTTCCAGGCCAGCAGCCAGGCC
TTGTGCCGGAGCTGGGTGGACACTATTTACAACGCACAGAACCAGCTGCAGCAGCTGCGT
GCACAGCTCAGTGCACAGGAGCACCCAGGCAGCCAGCATCTGCAGAGCCTGGAAGAGGAG
GAAGATGAGCAGGAGGAGGAGGGTGAAGAGAGCGGTACCTCAGCTGCCAGCTCCCCCACC
ATCCTTCGAAAGAGCAGCAACAGCCTCGACTCTGAGCACTGCACCTCAGATGGCTCCACG
GAGACCCTGGCCATGGTTGTGGTAGAGCCTGGGGCGACACTGTCTTCCCCGGAGTTTGAA
GGCGGTCCCGTCAGCTCCCAGTCGGACGAATCTTCTCTCAGTAACACTGCTTCCTCTGTC
ACTCCCACCAGCGAGCTGCTGCCCCTGGGCCCTGTGGATGGCCGCTCCTGCTCCATGGAC
TCCGCCTATGGCACCCTGTCCCCCACTTCCCTTCAAGACTTTGTGGCTCCACACCCCGTG
GTCGAGCCAGCGCCTGTGCCTCAGACCCCCTCTCCCCAGCCCTCACCCCGCCTCCGCCGT
CGCACTCCTGTCCAGCTGCTCCCTCGCCCGCCCCGCCTGCTCAAGTCCAAATCGGAGGCT
AGCCTCTTACAATTACTGTCAGGAACCCCAGCCGCCCGTGGCGTGCCTCCGGCTCCCAGC
CGCAGCCTGTCAGAACTGTGCCTGATCTCTGTGGCCCCTGGGGTTAGGACTCAGCGCCCC
CTTCAGGAAGGTGGTCCTGGCTGGAATGGTCCAGGGATGTGTGACCCCTGCCATGGCCCT
CAGTTGTCAGAATCTGAGAACAGACCTAGCCACATGACGGGGGGACCTGCAGATTCTGCC
CGAAGGAGATGCAGAGAGATGCCTTCTGGGACTATGTCCAGGGTGCAGTCTGAGCCTCCC
TCAGGGGTCTCTGCTCAACACAGGAAGCTGACCCTGGCCCAGCTCTACCGGATCAGGACC
ACCCTGCTGCTCAACTCCACGCTCACTGCCTCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3285
Start on reference sequence 70
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Plekhg5
Symbol Nomenclature : Plekhg5
SYNONYM : BC023181; Syx
Designation : PH domain-containing family G member 5|RhoA-specific guanine exchange factor SYX1|RhoA-specific guanine exchange factor SYX2|pleckstrin homology domain-containing family G member 5|synectin-binding RhoA exchange factor
Full Nomenclature : pleckstrin homology domain containing, family G (with RhoGef domain) member 5
GENEID : 269608
Locus Tag : RP23-284C20.1
GI : 151556611
GenBank Accession : BC148338
Mouse Genome Informatics : 2652860
Vega : OTTMUSG00000010225
Target GenBank: NM_001004156

 

Clone

Cloning Information : 265557
HIP Master Clone ID : 197655
IMAGE ID : 100015302
Original Clone ID : FLH265557.01F

Organism


TAX_ID : 10090
Species Specific ID: 269608

Genome

Chromosome : 4
Map Location : 4 E2|4
Ensembl : ENSMUSG00000039713

Reagents

Labome : Plekhg5-antibody