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Dnmbp (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Dnmbp
Gene Name:  dynamin binding protein
Sequence             
Original Clone ID: FLH265562.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00080939

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 4740nts         Open reading frame : 1 to 4740

ATGGAGCCTGGATCCATGGTCCGAGCCATCTTTGACTTCTGCCCTAGCGTCTCAGAGGAA
CTGCCACTCTTCGTGGGGGACGTTATTGAGGTGCTGGCCGTGGTGGATGAATTTTGGCTC
CTAGGGAAGAAGGAAGATGTTACAGGACAGTTCCCCAGCAGTTTTGTGGAGATCGTGACA
ATTCCCAGTCTGAAAGAGGGCGAGAGGCTGTTTGTCTGCATCTGCGAATTCGTATCTCGA
GAGCTGAACAGTCTTTCCCTCCATCGGGGAGACCTGGTGATTCTCGACGACAGCGCTCCC
ACTGCAGGATGGCTGCAGGGCCGAAGCTGCTGGGGCGCTTGGGGCTTCTTCCCATCCTCC
TGTGTCCAGGAGCTCTGCCTCTCCTCTCGGAGCCGACGCTGGCACGCACAGAGTGCGTTG
CTTCAGGCCCCAGAGTACTCCCTGGGACAAGCACGGGCCCTCATGGGCCTTTCTGCCCAG
TTGGACGAGGAGTTGGATTTCAGGGAAGGAGACCTGATCACCATTATCGGGGTTCCTGAG
CCGGGCTGGTTTGAGGGAGAGTTAGAGGGTCGGAGGGGCATTTTCCCAGAAGGTTTTGTA
GAGCTGCTGGGGCCCCTAAGGACTGTGGATGAGTCCGTAAACTCCAGAAGTGGAGATGAC
TCCGCTGTCAACGGTGAGGTGGATGTCCCTCCAGAAGAAGCAGAGAGTGGAGGCGACGAG
GACGACCAGCAGTCGGGGACCTATGGAATCGCCCTCTACAGATTCCAAGCCCTGGAGACG
AATGAGTTGGACTTTGAGGTAGGGGACAGAATTCAAATACTGGGGACCTTGGAGGATGGC
TGGCTGGAAGGATGTCTGAAGGGCAAGACAGGGGTCTTTCCCCATCGCTTTGTGAAGTTA
TGTCCTAGCAACAGGACAGAGGAAACCACGGCTCAGCCCCAGGAAAGCAGTTTCCCCAAG
GACTCTGAAAGCTCTGTGGGCAAGTCAGGGGACTCCGTGGTGGAGGAAGCAAGACAGGAA
CCATGGGAGTGTGAAGAAGAAAGACCTGACTATGATCTCCCAGGACAAGCCTCCGTGCCC
CAAGATCACGTAGCACCCGAGTGGACTGGAGATACGATCTCAGGTCAGGATAAAGATGCT
TCAGGGAGCTCTCCTGATGTGGATCTTGAAAGGCCTCTTGCTAAAGACCTTTCCACACCT
GACCCCTCAGAGGAAGTCAACGGTGTTTCTTCCCAGCCTCAGGTACCTATTCATCCCAAA
GTGCAGAAGAGCCAACACTATCTAACAGCAGGAGGGAGCCACCAAACCTCTGACCCATTC
TCTGAGCTTGTCCCACTAGAAGCCAGGACCAGAGACTACTCCAGCCTGCCTCCTAGAAGA
ACATACGCCCAAGGTTGGTCATTCCAGAAGCCAGCGTCCCACCTCCAGAGGGCCTCTTCC
CTTACAGCCTCAAGGCTAGACAGACCCAGCCACTTTTGCCATCCAGCCATGGCCAGCTAT
GCTCAGAAGCACCAAACATCTACAGAAAATACTGCCAGCCTCCACGATCCACCAGAGAGA
CCAGAGAGAAGGCCTGGCTTACAAGACAGGGGGCCTGCCACAGATATAACCACAGCGTCA
CAGGGGGACAGCCTGGACCTAGATTCCAAGTTGACCCAACAGCTGATAGAGTTTGAGAAG
AGTCTGTCAGGGCCCAGCACAGAGCCAGAGACGATTGTACGCCGCTTTTCAATCATGGAC
TTTTACTCTGAGAAGGATATCGTGAGAGGCTCCTCAAATTCACTACCCTCACAGGCTTTC
CCCGAGAGGAGAAAGACCCTGAGGCCACCACCTCCTAGACCCCGTACCCCAACACCCATT
TCCTCCCACTTGCTAGTTGACCAGAGCCCGAAACCTGTACCCACCTTGGTTGTGCGGCCC
TCCCGACCAGCTCCTCTGCCTCCTCCTGCCCAGCAGAGGATGAATACGGCCTCCCCCAAG
CCCACCTCCTGTGCCCATCCTGGCTGGGAGGCCCCAGAGAAGGAGGACTCTGAGCACATG
GAGAAGAGCCCAGCCCAGACTTTTCCGTGCCCCTCCATGCTGGCAAGGATCCGGGACGTG
GAACAGGACCTGGACACGTGTACCAGGGCTCAGGAAGAGCTGAACTTGCTGTTGGAGGAA
AAGCAAGATGACCCGTCGAGGGCAGAGACTCTTGAGACTCTCAGATCCTACGAGAGCACC
ATTCAGAGCCTGACCCTGGAGCTTCAGCAGCTGAGAGACATGACGCTCCTCTCTTCCCAG
TCTTCATCCCTGGCGGCCCCCTTTGGGTCTGTGTCCACTGAAAACCCAGAGCAGAGGATG
CTGGAGAAGAGAGCCAAGGTGGTGGCGGAGCTTTTGCAGACGGAGAGAGACTACATCCGG
GATCTGGAGATGTGCATTGAGAGGGTCATGGTGCCCCTGCAGCAGGCACAGGTACCAAAC
GTTGATTTTGAAGGACTTTTTGGAAATATGCAAACAGTGATTAAAGTCTCAAAGCAATTG
TTGGCGGCCTTGGAAATCAGCGATGCTGTAGGTATGAGCTCATGCGATTGTTTGGTCCCA
GGACCCGTGTTTCTTGACCACCGAGACGAGCTTGAGGGAACGTACAGGGTTTACTGCCAG
AACCACGATGAAGCCATCTCCCTGCTGGAAATGTATGAGAAGGACGAGAAGACCCAGAAG
CACCTTCAGGACTACCTGGCAGATCTCAAGGGATGCACCAATTACATCAACCTGGGCTCC
TTCCTCATCAAGCCCGTACAGAGAATAATGCGTTACCCGCTGCTGCTGATGGAGTTGCTG
AATTCCACCCCAGAATCCCACCCGGATAAAGTGCCTTTAACCAACGCGGTCCTCGCCGTC
AAGGAGATCAATGTTAACATTAATGAGTACAAGCGTCGGAAGGATCTGGTGCTTAAGTAC
CGCAAAGGGGATGAAGATAGCCTGATGGAAAAGATCTCCAAGCTGAACATACACTCCATC
ATCAAGAAGTCCAGCCGCGTTAGCAGCCACCTCAAACACCTCACTGGCTTCGCTCCGCAG
CTAAAAGATGAAGTATTTGAAGAGACAGAAAAGAACTTCCGGATGCAGGAGAGATTGATT
AAATCTTTTATCCGAGACCTGTCCCTCTACCTCCAGCATATACGGGAATCTGCATGTGTG
AAGGTGGTGGCAGCCATGAGCATATGGGATCTGTGCATGGAGAGGGGACATCATGACCTG
GAGCAGTTCGAGAAGGTGCATCGCTACATCAGTGACCAGCTCTTCACACGGTTTAAAGAG
AGGACCGAGCGGCTCGTCATCAATCCCTTAAACCAGTTGTTGAACATGTTCACGGGGCCC
TACAAGCTGGTGCAGAAGCGGTTCGACAAGCTCCTGGACTTCTACAACTGCACTGAGCGA
GCGGAGAAGCTGAAGGACAAGAAGACTCTGGAGGAGCTGCAGTCAGCCCGAAACAACTAC
GAGGCCCTGAACTCGCAGCTGCTGGACGAGCTGCCCAAGTTCCAGCAGTACGCCCAGAGC
CTCTTCACCAACTGCATCCACGGCTATGCCGAAGCCCACTGTGACTTCGTGCAGCAAGCC
CTGGAGCAGCTGCAGCCGCTGCTGTCGTTACTGAAAGCCACCGACCGAGAAGGCAACTTG
ATCGCCATCTTCCTTGAGGAGCACAGCCGGGTGCTACAGCAGCTCCAGGTCTTCACATTC
TTCCCCGAGTCCCTCCCAGCGCCCAGGAAGCCCTTTGAGAGGAAAACCACAGACCGCCAG
TCATCCCGGAAGACCCTTCTGGGCATGCCAAGCTACATGCTGCAGTCGGAAGAGCTGCGG
AGCTCCCTGCTGGCGAGGTACCCGCCGGAAAAGCTCTTCCACGTCCAGCGGAACTTCAAC
GCTGCGCAGGACTTGGATGTCTCCCTTTTGGAAGGCGACCTGGTGGGCGTGATCAAAAAG
AAAGACCCCATGGGCAGCCAGAACCGCTGGCTCGTGGACAACGGAGTCACCAAAGGCTTT
GTCTACAGCTCCTTCCTGAAGCCTTACAATCCTCGCTGCAGCCATTCGGATGCCTCCGTG
GCCAGCCACTCCTCCACGGAGTCAGAGCACAGTGGCTCCTCCCCCGGGTGCCATCGACAG
AACAGCCACAGTGCCTTGACCTTCAACTCCAACAACATGACTGTGTCCTTCACCTCAGGA
CTTGCCCTAACGCAGCCTCAGGATGCATCTCCACTGAAGGACTGTGCCCACGAAACTCTC
GCTGTGTCCTGGAACACAGGGCATCCAGAGACTGGACCTTCCACGTGCTCTTCCGACCCA
GGCTTTTCCTGCCAGCGCAGGCTGGGGAACCCTGCAGATGGTGCCAGAGACATCAGCCAA
CCTGCTTCAACCTTGAGGGGCTGCCAGCGAGGCTCCCCGCATTCAGAAGTGGTCGGTTAC
TCTGTGCCAGGGCAAAATGACCAAGGCAGTGACTCTATAAAAGGCTCTGCAAGAGTCTGT
CAGGCTCCAGAAGACAGAGACAGAGGGGTCGGGAGCAGCGAGACAGAGGGCAACCAGGTC
TATTTTGCCATTTATACTTTCAAGGCACGAAACCCAAATGAACTGAGTGTGTTAGCCAAT
CAGAGACTCAGGATCCATGAGTTTAAAGACGTCACAGGCAATACAGAGTGGTGGTTGGCT
GAAGTTAACGGAAGGAAGGGCTACGTCCCATCCAACTATATCCGGAAAACCGAGTACACC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4761
Start on reference sequence 19
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Dnmbp
Symbol Nomenclature : Dnmbp
SYNONYM : 2410003L07Rik; 2410003M15Rik; TUBA
Designation : dynamin-binding protein|scaffold protein Tuba
Full Nomenclature : dynamin binding protein
GENEID : 71972
GI : 151555198
GenBank Accession : BC148369
Mouse Genome Informatics : 1917352
Target GenBank: NM_028029

 

Clone

Cloning Information : 265562
HIP Master Clone ID : 197660
IMAGE ID : 100015342
Original Clone ID : FLH265562.01F

Organism


TAX_ID : 10090
Species Specific ID: 71972

Genome

Chromosome : 19
Map Location : 19|19 D1

Reagents

Labome : Dnmbp-antibody