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Ppef1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Ppef1
Gene Name:  protein phosphatase with EF hand calcium-binding domain 1
Sequence             
Original Clone ID: FLH265574.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00080951

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1950nts         Open reading frame : 1 to 1950

ATGGGCTGTGGCACTTCTTCAAAAAAAGGCAACAAATCTAAGAAAGTGATAAAAGCCGCG
TTAGTCATCCAAAATTGGTACCGGCGGTACAGAGCTCGACTGAGGGTCAGACAGCATTAT
GCCCTCGCCATCTTCCAGTCTATTGAATATTCTGACGAGCAAGGCCAAATGCAGCTATCC
AGCTTCTTTTCGTTCATGTTGGAAAACTACACAAAGACAAATAATGAAGATTCAGCATTA
GTAACTCGAATTTTTGACAACACCAGGCGGGAAAGCCAAATCAAGGATAGAGATGACTTT
TTGGGGTTGATAGAAGTGCCTGACTCCTATGATGGTCCTCGGCTGCAGTTTCCTCTGACG
TTTACTGACATCCATATACTTCTTCAGGCCTTCAAGCAACAGCAGATACTTCATGCTCAC
TACGTCTTAGAGGTGCTATTTGAAGCTAGGAAAGTCCTGAAGCAAATGCCGAATTTCTCT
CATGTAAAAACCTTTCCCGCCAAAGAGATAACAATATGTGGTGATTTGCATGGGAAACTG
GATGATCTTATGCTGATTTTCTATAAGAATGGCCTGCCTTCAGAGAACAATCCATACGTT
TTCAATGGTGACTTTGTAGATCGAGGAAACAATTCCATGGAGATCCTAATGATCCTGCTT
GTTTGTTTTCTTGTCTACCCCTCTGACCTGCACCTCAATAGAGGGAACCATGAAGACTTT
ATGATGAACCTGAGGTATGGCTTCACAAAAGAAATTTTACAAAAATACAAGCTACACGGG
AGAAAAATCTTACAAGTCTTGGAAGAAGTCTATACCTGGCTCCCAATCGGTACAATTATT
GACAACGAAATCCTGGTCATACATGGTGGGATATCAGAATCCACAGACTTGAATACACTC
CACCAGTTACAAAGAAATAAAATGAAATCTGTGTTGATGCCGCCAGTGTTGGGAAATCAA
GAGACTGGCGAAAAGAGAAATAAATCAGCGTCAAATTATGTTGAACCAAGGAAGGTTGAA
CCAGACAAGACTCCTTCTGAAGACTTGACAAAGCAAGAATGGGAACAAATAGTTGACATT
CTGTGGAGTGACCCCAGAGGCAAAAAAGGCTGTTATCCAAACACAAGCCGAGGAGGGGGC
TGCTATTTTGGACCAGATGTTACCTCTAAGGTTCTTAGTAAAAACCAGTTGAAGATGCTC
ATTAGGTCTCATGAGTGTAAACCCGACGGGTATGAAGTCTCTCATGATGGAAAGGTCATT
ACTGTATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAACCGAGGAGCTTACATCAGA
CTAAGTTATGGTACAATGCCACAATTTTTCCAGTACCAAGTAACTAGTACATCATGCCTG
AACCCTCTTCACCAAAGGATGAATGCTATGGAATCCAGTGCTTTCAAGATACTAAAGGAA
AAAATGATTTCACGAAAAACTGATCTCATCAACGCTTTCGAGCTCCGTGACCACAGTAGG
TCAGGAAGGATTTCATTGGCCGAGTGGGCATTTTCTATGGAGAACATTTTGGGGCTGAAC
TTACCATGGAGATCTCTAAGTTCACACCTGGTAACCATTGATTCCAGCGGAAGTGTTGAC
TACATGTCCAGCTTTGACGATATCCGTATTGAAAAGCCTACGAAAGATATGAAGTCCAAT
CTAACTGAAACTATGTACAGATACCGATCTGACCTGAAAATCATATTTAATATCATCGAC
AGTGATCAATCAGGTCTGATCTCCATGGATGAATTTCGGACCATGTGGAAACTTTTCAAT
GCTCATTACAAAGCTCATATAGATGATTCCCAAATTGATGAGCTTGCCAGCATAGTGGAC
TTCAACAAAGATGGCAACATCGACTTTAATGAGTTTTTAAAGGCTTTCTATGTAGTACAT
AAATATGATAAGCCAGGGACCAGTCTTGCT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2073
Start on reference sequence 121
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Ppef1
Symbol Nomenclature : Ppef1
SYNONYM : PPEF-1
Designation : DRES10|PPEF 1|protein phosphatase with EF calcium-binding domain|serine/threonine-protein phosphatase with EF-hands 1
Full Nomenclature : protein phosphatase with EF hand calcium-binding domain 1
GENEID : 237178
Locus Tag : RP23-263P17.1
GI : 151556657
GenBank Accession : BC148447
Mouse Genome Informatics : 1097157
Vega : OTTMUSG00000019590
Target GenBank: NM_011147

 

Clone

Cloning Information : 265574
HIP Master Clone ID : 197672
IMAGE ID : 100015437
Original Clone ID : FLH265574.01F

Organism


TAX_ID : 10090
Species Specific ID: 237178

Genome

Chromosome : X
Map Location : X F4|X 73.95 cM
Ensembl : ENSMUSG00000062168

Reagents

Labome : Ppef1-antibody