DNASU Plasmid Repository • 480.965.5697 | Email

Baz2a (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  Baz2a
Gene Name:  bromodomain adjacent to zinc finger domain, 2A
Sequence             
Original Clone ID: FLH265623.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00081000

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 5661nts         Open reading frame : 1 to 5661

ATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCTCCTGCACCAGCTGCCTCAGGA
CTGAAACCCTCTCCTTCCTCGGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTC
CCCCAGCAAGGGAAAAGTTTGAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCT
CACACTACTACTTCAGGGATTTTGAACTCTGCTCCCCACTCCTCTAGCACCTCACACCTC
CATCACCCTAACGTGGCCTACGACTGTCTTTGGAACTACTCACAGTACCCATCTGCCAAT
CCTGGCAACAACCTCAAGGACCCACCCCTTCTTTCTCAGTTCCCTGGGGGACAATACCCG
CTCAACGGTATCCTTGGGGGCAACCGACAACCTTCATCCCCAAGTCACAACACTAATCTT
CGAGCTGGGAGCCAAGAGTTCTGGGCCAATGGTACCCAGAGTCCCATGGGGCTTAACTTC
GATTCACAGGAACTGTATGATTCTTTTCCTGATCAGAATTTTGAGGTGATGCCCAATGGA
CCCCCAAGTTTTTTCACCTCCCCTCAGACTTCTCCAATGTTGGGGTCTAGTATCCAGACC
TTTGCACCTTCCCAGGATGTAAGCAGTGACATCCATCCTGATGAAGCAGCAGAAAAGGAG
CTGACTTCAGTTGTGGCAGAAAATGGCACTGGCTTGGTAGGCAGCCTGGAGCTGGAGGAA
GAGCAGCCAGAACTAAAGATGTGTGGCTACAATGGTTCTGTCTCCTCTGTGGAGTCTTTA
CACCAAGAAGTCTCCGTCCTGGTCCCTGATCCCACAGTGAGCTGTCTAGATGATCCTTCA
CATCTTCCTGATCAACTGGAAGACACTCCAATTCTCAGTGAAGACTCCCTGGAGCCCTTT
GACTCTCTGGCAGCAGAGCCAGTGAGTGGCAGTCTTTATGGTATAGATGATGCGGAGCTG
ATGGGTGCAGAAGACAAGTTGCCTCTGGAGGGCAACCCTGTGATCTCTGCCCTCGATTGC
CCTGCTCTCAGTAATGCTAATGCCTTCAGTCTCCTGGCAGACGACAGCCAGACATCAGCC
TCCATCTTTGTCAGCCCTACCTCCCCACCTGTCTTAGGGGAGTCTGTCTTGCAAGATAAC
AGCTTTGGACTGAACAGTTGCAGTGACTCTGAACAGGAAGAAATAGAGACCCAGTCTTCA
AACTTCCAACGTCCCCTGACTGAGCCAGCTCCTGACCAGCCACCTAGTACTCAACTACAT
CCAGCAGTTTCACCAACAGCCTCCCCAGCAGCCTCCTTGACAGCATCTGCAGAAATCTCT
CCAGCTGTCTCTCCAGTAGCATCCTCGCCTGTCCCTCCTGAAGTCTTTGTAGCAGTCTCT
CCAGCTTCCTCACCTGCTCTGCCAGCCATCTCTTTGGAAGCCTCTATGACAACTCCAGTA
ACTTCTCCTCAAGGTTCCCCTGAACCTTCTCCAGCAGCTGCCTTCCAGACTGTCTCCCCA
GCAAGGAAAAATGTCAGCAGTGCTCCTAAAGCGCGTGCTGATCGAGAAGAGACGACTGGA
GGAGCAGTTGCAGTCTCTGGTAGTGGTGATGTACTGAAGAGACGTATTGCTACCCCAGAA
GAAGTTCGTCTTCCCCTCCAGCATGGGTGGCGAAGAGAAGTGCGCATCAAGAAGGGCAGC
CATCGGTGGCAGGGGGAGACTTGGTACTATGGCCCCTGTGGGAAGAGAATGAAGCAATTT
CCAGAAGTTATCAAGTACCTGAGCCGAAATGTGGTGCACAGTGTCCGCCGTGAGCACTTC
AGCTTCAGTCCCCGCATGCCTGTTGGAGATTTCTTTGAAGAAAGAGATACACCAGAGGGC
TTGCAGTGGGTCCAGTTATCAGCAGAGGAGATTCCTTCCAGAATTCAAGCAATCACTGGC
AAACGAGGCCGACCTCGAAACAATGAGAAGGCTAAGAACAAGGAAGTTCCCAAAGTGAAG
CGGGGCCGAGGTCGGCCTCCTAAGATCAAAATGCCTGAGCTGTTGAATAAAACAGATAAC
CGACTTCCAAAGAAACTGGAAACCCAAGAAATACTGAGTGAGGACGATAAAGCAAAGATG
ACTAAAAACAAAAAGAAGATGAGGCAGAAGGTCCAACGGGGAGAAAGTCAGACTCCTGTC
CAAGGGCAGGCCAGAAACAAGAGGAAGCAAGACACCAAGAGCTTGAAGCAGAAGGACACT
AAGAAGAAATTGAAGGCTGAGAAAGAGAAGATGAAGACAAAGCAGGAAAAGCTGAAGGAA
AAGGTAAAGCGAGAAAAGAAAGAAAAGGTAAAAGCGAAGGGGAAGGAAGGGCCCAGAGCC
AGGCCATCCTGTAGAGCAGACAAGACCCTTGCCACACAGAAGCGGCTAGAGGAGCAGCAG
AGGCAGCAGGCTATCCTGGAGGAGATGAAGAAGCCCACAGAGGATATGTGTCTGTCTGAC
CACCAGCCCCTGCCTGACTTCACACGCATCCCTGGTTTGACACTGTCCAGTAGGGCTTTC
TCAGATTGCTTGACCATCGTGGAGTTCCTTCACAGTTTTGGCAAAGTGCTAGGCTTTGAC
CTTACCAAAGATGTTCCTAGTCTAGGAGTCCTGCAGGAGGGACTCTTATGTCAAGGTGAC
AGCTTGGACAAAGTGCAGGACCTGCTGGTGCGACTGCTGAAGGCTGCACTCCATGATCCT
GGTCTGCCCCCCTACTGTCAGTCCCTGAAGATATTGGGGGAGAAGATGTCAGAGATCCCA
TTGACCAGAGATAATGTGTCTGAGATACTGCGCTGCTTCCTCATGGCATATAGAGTGGAG
CCATCCTTCTGTGACAGTCTGCGTACCCAGCCTTTTCAGGCCCAGCCACCTCAACAGAAG
GCTGCTATTCTAGCCTTCCTTGTGCATGAGCTTAACAGCTCCACCATTATCATCAATGAG
ATTGACAAGACTCTGGAAAGTGTGTCTAGCTGCAGGAAGAACAAGTGGATTGTTGAAGGC
CGACTCCGGAGACTGAAAACTGCTCTGGCCAAGCGAACTGGGCGGCCTGAGGTTATGATG
GAAGGGGCAGAAGACGGCCTAGGACGGAGGCGCAGTTCTCGGATCATGGAGGAAACCAGT
GGCATAGAAGAGGAGGAAGAGGAAGAAAATACAACAGCTGTCCATGGCCGCAGGGGTCGA
AAAGAAGGAGAGATTGATGTTGCAGCATCTAGCATTCCAGAGCTAGAGCGCCATATAGAA
AAACTCAGTAAGCGTCAGCTCTTCTTTAGAAAAAAGCTGCTTCACTCATCCCAGATGCTT
CGGGCAGTGTCCTTGGGTCAAGACCGCTATAGACGCCATTACTGGGTATTACCGTATCTT
GCTGGTATCTTTGTGGAAGGATCGGAAGGGAGCACAGTTACTGAAGATGAAATAAAGCAA
GAAACTGAGTCCTTGATGGAAGTAGTCACTTCAACACCCAGCTCTGCCCGAGCCTCTGTA
AAGAGAGAATTAACTGGCTCCAATGCCTCTACTTCTCCTGCCCGGTCCCGAGGCCGACCT
CGAAAACCTAAGCCTGGGTCTCTGCAGCCTCAGCACCTTCAGTCCACCATTAGGGAATGT
GATTCAGAGCAAGCCCAGACTCAAGTTCACCCAGAACCCCAGCCTCAGCTTCAGGCCCCT
ACCCAGCCCCATCTTCAGCCAAGTAGTGGGTTCCTAGAGCCAGAAGGTTCCCCTTTCTCT
CTGGGTCAGAGCCAGCATGACCTCAGCCAGTCTGCCTTCTTGTCTTGGCTGAGCCAGACT
CAGAGCCACAACTCCCTGTTGAGCAGCTCAGTCCTCACGCCGGACAGCAGCCCAGGGAAA
CTAGACTCTGCTCCGTCTCAGTCCTTGGAGGAGCCCGAGCCTGATGAGGCTCAGTCCTGC
CCTGGTCCTCAAGGTCCCTGGTTTAACTTCTCAGCCCAGATACCCTGTGATGCTGCTCCT
ACACCACCTCCTGCAGTCTCTGAGGACCAACCTACTCCCTCCCTCCAGCTGCTGGCCTCC
TCTAAACCAATGAATACACCCGGTGCTGCCAATCCTTGTTCCCCAGTGCAGCTCTCTTCC
ACTCACTTGCCTGGAGGGACCCCTAAGAGGCTATCAGGGGACTCTGAAGAAATGTCACAG
AGTCCCACTGGGCTGGGGCAACCAAAGCGGAGGGGGAGACCCCCTAGCAAGTTCTTCAAG
CAGGTGGAGCAGCATTACTTAACCCAGCTGACAGCCCAGCCTATCCCCCCTGAGATGTGC
TCGGGCTGGTGGTGGATCCGAGACCCTGAGACACTGGATGTCCTGCTCAAGGCACTGCAT
CCCCGAGGCATCCGGGAGAAGGCGCTTCACAAACATCTTAGCAAGCACAAGGACTTTTTG
CAGGAAGTTTGTTTACAGCCCTTAACTGATCCCATCTTTGAGCCTAATGAGCTCCCTGCC
TTGGAAGAAGGCGTTATGAGCTGGTCCCCCAAAGAGAAGACGTACGAGACAGACCTAGCT
GTGCTCCAGTGGGTGGAGGAGCTGGAGCAGCGGGTTGTCCTCTCCGATCTGCAGATTCGG
GGCTGGACATGCCCTACCCCAGACTCCACCAGAGAAGACTTGACCTACTGTGAGCATCTG
CCTGACTCCCCGGAGGATATCCCTTGGAGGGGTCGGGGCAGGGAAGGAACAGTACCTCAG
CGGCAGAACAACAACCCTCTGGACCTCGCTGTGATGCGATTGGCTGTTCTGGAGCAAAAT
GTGGAGCGGCGGTACTTGCGGGAGCCCCTCTGGGCAGCCCATGAGGTGGTAGTGGAGAAG
GCCCTACTGAGCACACCCAATGGTGCCCCTGATGGCACCTCAACTGAGATATCCTATGAG
ATCACCCCTCGTGTCCGAGTTTGGCGGCAGACACTTGAAAGGTGCCGTAGTGCAGCCCAA
GTGTGCTTGTGCATGGGCCAGCTAGAAAGGTCCATCGCGTGGGAGAAGTCTGTCAACAAA
GTGACCTGCCTGGTCTGCCGGAAGGGCGATAATGATGAGTTTCTCCTGCTGTGTGATGGG
TGTGACCGAGGCTGCCACATTTACTGTCATCGGCCCAAGATGGAGGCTGTTCCAGAAGGA
GATTGGTTCTGTGCTGTCTGTCTGTCCCAGCAGGTAGAGGAAGAGTACACTCAGAGGCCT
GGTTTTCCAAAACGAGGTCAGAAGCGGAAAAGTAGTTTTCCACTGACCTTCCCAGAAGGT
GACAGCCGGCGGCGGATGTTGTCAAGGAGCCGAGATAGTCCAGCAGTGCCTCGGTACCCA
GAAGACGGGCTGTCTCCCCCCAAAAGACGGCGACATTCGATGAGAAGTCACCACAGTGAT
CTCACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCTTTC
CTAGAGCCTGTGAACCCTCGCTTGGTGAGTGGATACCGACGTGTCATCAAGAACCCTATG
GATTTTTCCACCATGCGAGAACGCCTGCTCCGTGGAGGGTACACTAGCTCAGAAGAGTTT
GCAGCTGATGCTCTGCTGGTTTTTGACAACTGCCAGACCTTCAATGAGGATGACTCTGAA
GTGGGCAAGGCTGGGCACGTCATGCGACGCTTCTTTGAGAGCCGCTGGGAGGAATTTTAT
CAGGGAAAACAGGCCAATCTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 5819
Start on reference sequence 156
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Baz2a
Symbol Nomenclature : Baz2a
SYNONYM : AA415431; C030005G16Rik; C78388; Tip5; Walp3; mKIAA0314
Designation : TTF-I-interacting protein 5|bromodomain adjacent to zinc finger domain protein 2A|transcription termination factor I-interacting protein 5
Full Nomenclature : bromodomain adjacent to zinc finger domain, 2A
GENEID : 116848
GI : 151555256
GenBank Accession : BC148495
Mouse Genome Informatics : 2151152
Target GenBank: NM_054078

 

Clone

Cloning Information : 265623
HIP Master Clone ID : 197721
IMAGE ID : 100015492
Original Clone ID : FLH265623.01F

Organism


TAX_ID : 10090
Species Specific ID: 116848

Genome

Chromosome : 10
Map Location : 10 D3|10
Ensembl : ENSMUSG00000040054

Reagents

Labome : Baz2a-antibody