Gene:
Gene Symbol:
SOS1
Gene Name:
son of sevenless homolog 1 (Drosophila)
Original Clone ID:
FLH265857.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081233
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAGGCGCAGCAGCTGCCCTACGAGTTTTTCAGCGAAGAGAACGCGCCCAAGTGGCGG
GGACTACTGGTGCCTGCGCTGAAAAAGGTCCAGGGGCAAGTTCATCCTACTCTCGAGTCT
AATGATGATGCTCTTCAGTATGTTGAAGAATTAATTTTGCAATTATTAAATATGCTATGC
CAAGCTCAGCCCCGAAGTGCTTCAGATGTAGAGGAACGTGTTCAAAAAAGTTTCCCTCAT
CCAATTGATAAATGGGCAATAGCTGATGCCCAATCAGCTATTGAAAAGAGGAAGCGAAGA
AACCCTTTATCTCTCCCAGTAGAAAAAATTCATCCTTTATTAAAGGAGGTCCTAGGTTAT
AAAATTGACCACCAGGTTTCTGTTTACATAGTAGCAGTCTTAGAATACATTTCTGCAGAC
ATTTTAAAGCTGGTTGGGAATTATGTAAGAAATATACGGCATTATGAAATTACAAAACAA
GATATTAAAGTGGCAATGTGTGCTGACAAGGTATTGATGGATATGTTTCATCAAGATGTA
GAAGATATTAATATATTATCTTTAACTGACGAAGAGCCTTCCACCTCAGGAGAACAAACT
TACTATGATTTGGTAAAAGCATTTATGGCAGAAATTCGACAATATATAAGGGAACTAAAT
CTAATTATAAAAGTTTTTAGAGAGCCCTTTGTCTCCAATTCAAAATTGTTTTCAGCTAAT
GATGTAGAAAATATATTTAGTCGCATAGTAGATATACATGAACTTAGTGTAAAGTTACTG
GGCCATATAGAAGATACAGTAGAAATGACAGATGAAGGCAGTCCCCATCCACTAGTAGGA
AGCTGCTTTGAAGACTTAGCAGAGGAACTGGCATTTGATCCATATGAATCGTATGCTCGA
GATATTTTGCGACCTGGTTTTCATGATCGTTTCCTTAGTCAGTTATCAAAGCCTGGGGCA
GCACTTTATTTGCAGTCAATAGGCGAAGGTTTCAAAGAAGCTGTTCAATATGTTTTACCC
AGGCTGCTTCTGGCCCCTGTTTACCACTGTCTCCATTACTTTGAACTTTTGAAGCAGTTA
GAAGAAAAAAGTGAAGATCAAGAAGACAAGGAATGTTTAAAACAAGCAATAACAGCTTTG
CTTAATGTTCAGAGTGGTATGGAAAAAATATGTTCTAAAAGTCTTGCAAAACGAAGACTG
AGTGAATCTGCATGTCGGTTTTATAGTCAGCAAATGAAGGGGAAACAACTAGCAATCAAG
AAGATGAACGAGATTCAGAAGAATATTGATGGTTGGGAGGGAAAAGACATTGGACAGTGT
TGTAATGAATTTATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACATGAGAGACAC
ATATTTCTCTTTGATGGCTTAATGATTTGCTGTAAATCAAATCATGGGCAGCCAAGACTT
CCTGGTGCTAGCAATGCAGAATATCGTCTTAAAGAAAAGTTTTTTATGCGAAAGGTACAA
ATTAATGATAAAGATGACACCAATGAATACAAGCATGCTTTTGAAATAATTTTAAAAGAT
GAAAATAGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAAAACAATTGGATGGCAGCA
TTGATATCTTTACAGTACCGGAGTACACTGGAAAGGATGCTTGATGTAACAATGCTACAG
GAAGAGAAAGAGGAGCAGATGAGGCTGCCTAGTGCTGATGTTTATAGATTTGCAGAGCCT
GACTCTGAAGAGAATATTATATTTGAAGAGAACATGCAGCCCAAGGCTGGAATTCCAATT
ATCAAAGCAGGAACTGTTATTAAACTTATAGAGAGGCTTACGTACCATATGTACGCAGAT
CCCAATTTTGTTCGGACATTTCTTACAACATACAGATCCTTTTGCAAACCTCAAGAACTA
CTGAGTCTTATAATAGAAAGGTTTGAAATTCCAGAGCCTGAGCCAACAGAAGCTGATCGC
ATAGCTATAGAGAATGGAGATCAACCCTTGAGTGCAGAACTGAAAAGATTTAGAAAAGAA
TATATACAGCCTGTGCAACTGCGAGTATTAAATGTATGTCGGCACTGGGTAGAGCACCAC
TTCTATGATTTTGAAAGAGATGCATATCTTTTGCAACGAATGGAAGAATTTATTGGAACA
GTAAGAGGTAAAGCAATGAAAAAATGGGTTGAATCCATCACTAAAATAATCCAAAGGAAA
AAAATTGCAAGAGACAATGGACCAGGTCATAATATTACATTTCAGAGTTCACCTCCCACA
GTTGAGTGGCATATAAGCAGACCTGGGCACATAGAGACTTTTGACCTGCTCACCTTACAC
CCAATAGAAATTGCTCGACAACTCACTTTACTTGAATCAGATCTATACCGAGCTGTACAG
CCATCAGAATTAGTTGGAAGTGTGTGGACAAAAGAAGACAAAGAAATTAACTCTCCTAAT
CTTCTGAAAATGATTCGACATACCACCAACCTCACTCTGTGGTTTGAGAAATGTATTGTA
GAAACTGAAAATTTAGAAGAAAGAGTAGCTGTGGTGAGTCGAATTATTGAGATTCTACAA
GTCTTTCAAGAGTTGAACAACTTTAATGGTGTCCTTGAGGTTGTCAGTGCTATGAATTCA
TCACCTGTTTACAGACTAGACCACACATTTGAGCAAATACCAAGTCGCCAGAAGAAAATT
TTAGAAGAAGCTCATGAATTGAGTGAAGATCACTATAAGAAATATTTGGCAAAACTCAGG
TCTATTAATCCACCATGTGTGCCTTTCTTTGGAATTTATCTCACTAATATCTTGAAAACA
GAAGAAGGCAACCCTGAGGTCCTAAAAAGACATGGAAAAGAGCTTATAAACTTTAGCAAA
AGGAGGAAAGTAGCAGAAATAACAGGAGAGATCCAGCAGTACCAAAATCAGCCTTACTGT
TTACGAGTAGAATCAGATATCAAAAGGTTCTTTGAAAACTTGAATCCGATGGGAAATAGC
ATGGAGAAGGAATTTACAGATTATCTTTTCAACAAATCCCTAGAAATAGAACCACGAAAC
CCTAAGCCTCTCCCAAGATTTCCAAAAAAATATAGCTATCCCCTAAAATCTCCTGGTGTT
CGTCCATCAAACCCAAGACCAGGTACCATGAGGCATCCCACACCTCTGCAGCAGGAGCCA
AGGAAAATTAGTTATAGTAGGATCCCTGAAAGTGAAACAGAAAGTACAGCATCTGCACCA
AATTCTCCAAGAACACCGTTAACACCTCCGCCTGCTTCTGGTGCTTCCAGTACCACAGAT
GTTTGCAGTGTATTTGATTCCGATCATTCGAGCCCTTTTCACTCAAGCAATGATACCGTC
TTTATCCAAGTTACTCTGCCCCATGGCCCAAGATCTGCTTCTGTATCATCTATAAGTTTA
ACCAAAGGCACTGATGAAGTGCCTGTCCCTCCTCCTGTTCCTCCACGAAGACGACCAGAA
TCTGCCCCAGCAGAATCTTCACCATCTAAGATTATGTCTAAGCATTTGGACAGTCCCCCA
GCCATTCCTCCTAGGCAACCCACATCAAAAGCCTATTCACCACGATATTCAATATCAGAC
CGGACCTCTATCTCAGACCCTCCTGAAAGCCCTCCCTTATTACCACCACGAGAACCTGTG
AGGACACCTGATGTTTTCTCAAGCTCACCACTACATCTCCAACCTCCCCCTTTGGGCAAA
AAAAGTGACCATGGCAATGCCTTCTTCCCAAACAGCCCTTCCCCCTTTACACCACCTCCT
CCTCAAACACCTTCTCCTCACGGCACAAGAAGGCATCTGCCATCACCACCATTGACACAA
GAAGTGGACCTTCATTCCATTGCTGGGCCGCCTGTTCCTCCACGACAAAGCACTTCTCAA
CATATCCCTAAACTCCCTCCAAAAACTTACAAAAGGGAGCACACACACCCATCCATGCAC
AGAGATGGACCACCACTGTTGGAGAATGCCCATTCTTCC
Protein sequence predicted based on nucleotide sequence
MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESNDDALQYVEELILQLLNMLC QAQPRSASDVEERVQKSFPHPIDKWAIADAQSAIEKRKRRNPLSLPVEKIHPLLKEVLGY KIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDV EDINILSLTDEEPSTSGEQTYYDLVKAFMAEIRQYIRELNLIIKVFREPFVSNSKLFSAN DVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYAR DILRPGFHDRFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQL EEKSEDQEDKECLKQAITALLNVQSGMEKICSKSLAKRRLSESACRFYSQQMKGKQLAIK KMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRL PGASNAEYRLKEKFFMRKVQINDKDDTNEYKHAFEIILKDENSVIFSAKSAEEKNNWMAA LISLQYRSTLERMLDVTMLQEEKEEQMRLPSADVYRFAEPDSEENIIFEENMQPKAGIPI IKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADR IAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDAYLLQRMEEFIGT VRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLH PIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIV ETENLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKI LEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSK RRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRN PKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESTASAP NSPRTPLTPPPASGASSTTDVCSVFDSDHSSPFHSSNDTVFIQVTLPHGPRSASVSSISL TKGTDEVPVPPPVPPRRRPESAPAESSPSKIMSKHLDSPPAIPPRQPTSKAYSPRYSISD RTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPP PQTPSPHGTRRHLPSPPLTQEVDLHSIAGPPVPPRQSTSQHIPKLPPKTYKREHTHPSMH RDGPPLLENAHSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4002
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
