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BPIL3 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB6
Gene Name:  BPI fold containing family B, member 6
Sequence             
Original Clone ID: FLH265868.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00081244

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1359nts         Open reading frame : 1 to 1359
Reference Sequence Alignment

ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCT
GGGGCACTGCTGCGGTTGGGCATGGACATCATGAACCAGGTCCAGAGCGCCATGGATGAG
AGTCATATCCTGGAGAAGATGGCAGCCGAGGCAGGCAAGAAACAGCCAGGGATGAAACCT
ATCAAGGGCATCACCAATTTGAAGGTGAAGGATGTCCAGCTGCCCGTCATCACACTGAAC
TTTGTACCTGGAGTGGGCATCTTCCAATGTGTGTCCACAGGCATGACCGTCACTGGCAAG
AGCTTCATGGGAGGGAACATGGAGATCATCGTGGCCCTGAACATCACAGCCACCAACCGG
CTTCTGCGGGATGAGGAGACAGGCCTCCCCGTGTTCAAGAGTGAGGGCTGTGAGGTCATC
CTGGTCAATGTGAAGACTAACCTGCCTAGCAACATGCTCCCCAAGATGGTCAACAAGTTC
CTGGACAGCACCCTGCACAAAGTCCTCCCTGGGCTGATGTGTCCCGCCATCGATGCAGTC
CTGGTGTATGTGAACAGGAAGTGGACCAACCTCAGTGACCCCATGCCTGTGGGCCAGATG
GGCACCGTCAAATATGTTCTGATGTCCGCACCAGCCACCACAGCCAGCTACATCCAACTG
GACTTCAGTCCTGTGGTGCAGCAGCAAAAGGGCAAAACCATCAAGCTTGCTGATGCCGGG
GAGGCCCTCACGTTCCCTGAGGGTTATGCCAAAGGCTCGTCGCAGCTGCTGCTCCCAGCC
ACCTTCCTCTCTGCAGAGCTTGCCCTTCTGCAGAAGTCCTTTCATGTGAATATCCAGGAT
ACAATGATTGGTGAGCTGCCCCCACAAACCACCAAGACCCTGGCTCGCTTCATTCCTGAA
GTGGCTGTAGCTTATCCCAAGTCAAAGCCCTTGACGACCCAGATCAAGATAAAGAAGCCT
CCCAAGGTCACTATGAAGACAGGCAAGAGCCTGCTGCACCTCCACAGCACCCTGGAGATG
TTCGCAGCTCGGTGGCGGAGCAAGGCTCCAATGTCCCTCTTTCTCCTAGAAGTGCACTTC
AATCTGAAGGTCCAGTACTCAGTGCACGAGAACCAGCTGCAGATGGCCACTTCTTTGGAC
AGATTACTGAGCTTGTCCCGGAAGTCCTCATCGATTGGCAACTTCAATGAGAGGGAATTA
ACTGGCTTCATCACCAGCTATCTCGAAGAAGCCTACATCCCAGTTGTCAATGATGTGCTT
CAAGTGGGGCTCCCACTCCCGGACTTTCTGGCCATGAATTACAATCTGGCTGAGCTGGAC
ATAGTAGAGAATGCCCTGATGCTGGACTTGAAGCTGGGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1362
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB6
Symbol Nomenclature : BPIFB6
SYNONYM : BPIL3; LPLUNC6
Designation : BPI fold-containing family B member 6|bactericidal/permeability-increasing protein-like 3
Full Nomenclature : BPI fold containing family B, member 6
GENEID : 128859
GI : 147898030
GenBank Accession : BC140306
HGNC : 16504
MIM : 614110
Vega : OTTHUMG00000032238
Target GenBank: NM_174897

 

Reference Sequence Alignment

  

HsCD00081244      ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCT
NM_174897.2       ATGCTGCGGATCCTGTGCCTGGCACTCTGCAGCCTGCTGACTGGCACGCGAGCTGACCCT
                  ************************************************************

HsCD00081244      GGGGCACTGCTGCGGTTGGGCATGGACATCATGAACCAGGTCCAGAGCGCCATGGATGAG
NM_174897.2       GGGGCACTGCTGCGGTTGGGCATGGACATCATGAACCAGGTCCAGAGCGCCATGGATGAG
                  ************************************************************

HsCD00081244      AGTCATATCCTGGAGAAGATGGCAGCCGAGGCAGGCAAGAAACAGCCAGGGATGAAACCT
NM_174897.2       AGTCATATCCTGGAGAAGATGGCAGCCGAGGCAGGCAAGAAACAGCCAGGGATGAAACCT
                  ************************************************************

HsCD00081244      ATCAAGGGCATCACCAATTTGAAGGTGAAGGATGTCCAGCTGCCCGTCATCACACTGAAC
NM_174897.2       ATCAAGGGCATCACCAATTTGAAGGTGAAGGATGTCCAGCTGCCCGTCATCACACTGAAC
                  ************************************************************

HsCD00081244      TTTGTACCTGGAGTGGGCATCTTCCAATGTGTGTCCACAGGCATGACCGTCACTGGCAAG
NM_174897.2       TTTGTACCTGGAGTGGGCATCTTCCAATGTGTGTCCACAGGCATGACCGTCACTGGCAAG
                  ************************************************************

HsCD00081244      AGCTTCATGGGAGGGAACATGGAGATCATCGTGGCCCTGAACATCACAGCCACCAACCGG
NM_174897.2       AGCTTCATGGGAGGGAACATGGAGATCATCGTGGCCCTGAACATCACAGCCACCAACCGG
                  ************************************************************

HsCD00081244      CTTCTGCGGGATGAGGAGACAGGCCTCCCCGTGTTCAAGAGTGAGGGCTGTGAGGTCATC
NM_174897.2       CTTCTGCGGGATGAGGAGACAGGCCTCCCCGTGTTCAAGAGTGAGGGCTGTGAGGTCATC
                  ************************************************************

HsCD00081244      CTGGTCAATGTGAAGACTAACCTGCCTAGCAACATGCTCCCCAAGATGGTCAACAAGTTC
NM_174897.2       CTGGTCAATGTGAAGACTAACCTGCCTAGCAACATGCTCCCCAAGATGGTCAACAAGTTC
                  ************************************************************

HsCD00081244      CTGGACAGCACCCTGCACAAAGTCCTCCCTGGGCTGATGTGTCCCGCCATCGATGCAGTC
NM_174897.2       CTGGACAGCACCCTGCACAAAGTCCTCCCTGGGCTGATGTGTCCCGCCATCGATGCAGTC
                  ************************************************************

HsCD00081244      CTGGTGTATGTGAACAGGAAGTGGACCAACCTCAGTGACCCCATGCCTGTGGGCCAGATG
NM_174897.2       CTGGTGTATGTGAACAGGAAGTGGACCAACCTCAGTGACCCCATGCCTGTGGGCCAGATG
                  ************************************************************

HsCD00081244      GGCACCGTCAAATATGTTCTGATGTCCGCACCAGCCACCACAGCCAGCTACATCCAACTG
NM_174897.2       GGCACCGTCAAATATGTTCTGATGTCCGCACCAGCCACCACAGCCAGCTACATCCAACTG
                  ************************************************************

HsCD00081244      GACTTCAGTCCTGTGGTGCAGCAGCAAAAGGGCAAAACCATCAAGCTTGCTGATGCCGGG
NM_174897.2       GACTTCAGTCCTGTGGTGCAGCAGCAAAAGGGCAAAACCATCAAGCTTGCTGATGCCGGG
                  ************************************************************

HsCD00081244      GAGGCCCTCACGTTCCCTGAGGGTTATGCCAAAGGCTCGTCGCAGCTGCTGCTCCCAGCC
NM_174897.2       GAGGCCCTCACGTTCCCTGAGGGTTATGCCAAAGGCTCGTCGCAGCTGCTGCTCCCAGCC
                  ************************************************************

HsCD00081244      ACCTTCCTCTCTGCAGAGCTTGCCCTTCTGCAGAAGTCCTTTCATGTGAATATCCAGGAT
NM_174897.2       ACCTTCCTCTCTGCAGAGCTTGCCCTTCTGCAGAAGTCCTTTCATGTGAATATCCAGGAT
                  ************************************************************

HsCD00081244      ACAATGATTGGTGAGCTGCCCCCACAAACCACCAAGACCCTGGCTCGCTTCATTCCTGAA
NM_174897.2       ACAATGATTGGTGAGCTGCCCCCACAAACCACCAAGACCCTGGCTCGCTTCATTCCTGAA
                  ************************************************************

HsCD00081244      GTGGCTGTAGCTTATCCCAAGTCAAAGCCCTTGACGACCCAGATCAAGATAAAGAAGCCT
NM_174897.2       GTGGCTGTAGCTTATCCCAAGTCAAAGCCCTTGACGACCCAGATCAAGATAAAGAAGCCT
                  ************************************************************

HsCD00081244      CCCAAGGTCACTATGAAGACAGGCAAGAGCCTGCTGCACCTCCACAGCACCCTGGAGATG
NM_174897.2       CCCAAGGTCACTATGAAGACAGGCAAGAGCCTGCTGCACCTCCACAGCACCCTGGAGATG
                  ************************************************************

HsCD00081244      TTCGCAGCTCGGTGGCGGAGCAAGGCTCCAATGTCCCTCTTTCTCCTAGAAGTGCACTTC
NM_174897.2       TTCGCAGCTCGGTGGCGGAGCAAGGCTCCAATGTCCCTCTTTCTCCTAGAAGTGCACTTC
                  ************************************************************

HsCD00081244      AATCTGAAGGTCCAGTACTCAGTGCACGAGAACCAGCTGCAGATGGCCACTTCTTTGGAC
NM_174897.2       AATCTGAAGGTCCAGTACTCAGTGCATGAGAACCAGCTGCAGATGGCCACTTCTTTGGAC
                  ************************** *********************************

HsCD00081244      AGATTACTGAGCTTGTCCCGGAAGTCCTCATCGATTGGCAACTTCAATGAGAGGGAATTA
NM_174897.2       AGATTACTGAGCTTGTCCCGGAAGTCCTCATCGATTGGCAACTTCAATGAGAGGGAATTA
                  ************************************************************

HsCD00081244      ACTGGCTTCATCACCAGCTATCTCGAAGAAGCCTACATCCCAGTTGTCAATGATGTGCTT
NM_174897.2       ACTGGCTTCATCACCAGCTATCTCGAAGAAGCCTACATCCCAGTTGTCAATGATGTGCTT
                  ************************************************************

HsCD00081244      CAAGTGGGGCTCCCACTCCCGGACTTTCTGGCCATGAATTACAATCTGGCTGAGCTGGAC
NM_174897.2       CAAGTGGGGCTCCCACTCCCGGACTTTCTGGCCATGAATTACAACCTGGCTGAGCTGGAC
                  ******************************************** ***************

HsCD00081244      ATAGTAGAGAATGCCCTGATGCTGGACTTGAAGCTGGGC---
NM_174897.2       ATAGTAGAGAATGCCCTGATGCTGGACTTGAAGCTGGGCTGA
                  ***************************************   

Protein

SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
UniProt : Q8NFQ5
HPRD : 16561

Clone

Cloning Information : 265868
HIP Master Clone ID : 197966
IMAGE ID : 100014317
Original Clone ID : FLH265868.01L

Organism


TAX_ID : 9606
Species Specific ID: 128859

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000167104

Reagents

Labome : BPIFB6-antibody