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Ear10 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Ear10
Gene Name:  eosinophil-associated, ribonuclease A family, member 10
Sequence             
Original Clone ID: FLH265961.01F
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00081337

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 468nts         Open reading frame : 1 to 468

ATGGGTCCAAAGCTGCTTGAGTCCCGAATTTGCCTCCTGCTGCTGCTAGGACTTGTCCTA
ATGCTTGCCTCATGCCTGGGACAAACCACTTCCCAGTGGTTTGCCATCCAGCATATCAAT
AATAATGCCAACCTCCAATGTAATGTTGAAATGCAGCGTATTAACAGGTTTAGAAGAACA
TGTAAGGGCTTAAATACTTTTCTTCATACAAGTTTTGCTAATGCTGTTGGTGTGTGTGGA
AATCCAAGTGGCTTGTGCAGTGACAATATAAGTCAAAACTGTCATAATAGTTCATATCGG
GTACATATAACTGTCTGTAACATCACCAGTTGGAGGAGAACACCTTATACCCAATGCAGA
TACCAAGCTAAAAGATCATTGGAGTACTACACAGTTGCCTGTGACCCCAGAACTCCACAG
GACAGTCCCATGTATCCAGTGGTTCCAGTTCACTTGGATGGGACATTT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 471
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Ear10
Symbol Nomenclature : Ear10
SYNONYM : Raf2
Designation : ribonuclease A f2
Full Nomenclature : eosinophil-associated, ribonuclease A family, member 10
GENEID : 93725
GI : 147898142
GenBank Accession : BC140220
Mouse Genome Informatics : 1890464
Target GenBank: NM_053112

 

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
UniProt : Q923L6

Clone

Cloning Information : 265961
HIP Master Clone ID : 198059
IMAGE ID : 100014257
Original Clone ID : FLH265961.01F

Organism


TAX_ID : 10090
Species Specific ID: 93725

Genome

Chromosome : 14
Map Location : 14 C1|14
Ensembl : ENSMUSG00000090166

Reagents

Labome : Ear10-siRNA