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Detailed Vector Information: pPPL

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pPPL              
Source: None
Description: ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
Comments: None
Publications: None
Authors: Debra T. Hansen
EvNO00813763

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AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
       DyNA Vector Map

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Vector Name: pPPL

pPPL in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: ligation independent cloning (LIC) clone with pBR322 origin and Ampicillin Resistance and ROP and ORF frame 2 Tetracycline resistance and ORF frame 1 and pBRrevBam and T7 and T7 term primer and BseRI Stuffer and MBP signal sequence (M1-A26 of NP_290668) and RBS and MBP (K27-T392 of NP_290668) and HIS10 and TEV and FC sequencing primer pET26 pelB seq R and FC sequencing primer MBP pelB seqF and FC sequencing primer pET-PPL seq 1 F and FC sequencing primer pET-PPL seq 1 R and FC sequencing primer pET-PPL seq 2 F and T7 term and FC sequencing primer pET-PPL seq 2 R and AmpR; ampicillin resistance in bacterial;
Comments: TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949
Size (bp): 6447
Parent Vector: Name: pET PPL His6 MBP LIC cloning vector (2K-T)
Description:
Properties: ligation independent cloning (LIC)
Author Name: Debra T. Hansen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
CDS ORF frame 1 3283 3741
CDS BseRI Stuffer 5456 5867
CDS Ampicillin Resistance 209 1069
CDS ORF frame 2 Tetracycline resistance 3088 3873
Miscellaneous Feature MBP signal sequence (M1-A26 of NP_290668) 4147 4224
Miscellaneous Feature ROP 2258 2449
Miscellaneous Feature TEV 5428 5445
Miscellaneous Feature MBP (K27-T392 of NP_290668) 4276 5373
Replication Origin pBR322 origin 1224 184
Terminator T7 term 5941 6069
primer T7 term primer 5983 6001
primer pBRrevBam 3907 3926
primer FC sequencing primer pET-PPL seq 2 R 6102 6122
primer FC sequencing primer pET-PPL seq 2 F 4678 4702
primer FC sequencing primer pET-PPL seq 1 R 5943 5964
primer FC sequencing primer pET-PPL seq 1 F 3979 4000
primer FC sequencing primer pET26 pelB seq R 6026 6047
primer FC sequencing primer MBP pelB seqF 5250 5274
promoter T7 4010 4028
promoter AmpR 139 167
ribosome binding site RBS 4130 4139
tag HIS10 4234 4263

 

Species Specific ID: None
Target GenBank: None