ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
ligation independent cloning (LIC) clone, bacterial expression expression with Operator O2 and araC promoter and T7 term and Operator O1 and B-lactamase gene and CAP site and T7 term primer and pBR322 origin and Operator I1 and I2 and arabinose BAD promoter and rrnB T1 T2 and RBS and ARAC3F Primer and PCBSREV Primer and ORICOL Primer and BADT1A Primer and BADT1B Primer and AMP5R Primer and -35 AP and F1ORI Primer and ARAC5R and -10 AP and rep_origin and ROP and araC; ampicillin resistance in bacterial;
Comments:
TEV protease cleavage site is designed for use with ligation-independent cloning into the BseRI site. This TEV site contains only the amino acids DNLYFQ. Cleavage occurs after the Q, and requires a compatible amino acid C-terminal to the Q, such as S, G, A, M. See Kapust et al 2002 Biochem Biophys Res Commun 294:949