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BAG2 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BAG2
Gene Name:  BCL2-associated athanogene 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266177.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23359685
Title: Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome
Authors: The ORFeome Collaboration
HsCD00081553

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 633nts         Open reading frame : 1 to 633
Reference Sequence Alignment

ATGGCTCAGGCGAAGATCAACGCTAAAGCCAACGAGGGGCGCTTCTGCCGCTCCTCCTCC
ATGGCTGACCGCTCCAGCCGCCTGCTGGAGAGCCTGGACCAGCTGGAGCTCAGGGTTGAA
GCTTTGAGAGAAGCAGCAACTGCTGTTGAGCAAGAGAAAGAAATCCTTCTGGAAATGATC
CACAGTATCCAAAATAGCCAGGACATGAGGCAGATCAGTGACGGAGAAAGAGAAGAATTA
AATCTGACTGCAAACCGTTTGATGGGAAGAACTCTCACCGTTGAAGTGTCAGTAGAAACA
ATTAGAAACCCCCAGCAGCAAGAATCCCTAAAGCATGCCACAAGGATTATTGATGAGGTG
GTCAATAAGTTTCTGGATGATTTGGGAAATGCCAAGAGTCATTTAATGTCGCTCTACAGT
GCATGTTCATCTGAGGTGCCACATGGGCCAGTTGATCAGAAGTTTCAATCCATAGTAATT
GGCTGTGCTCTTGAAGATCAGAAGAAAATTAAGAGAAGATTAGAGACTCTGCTTAGAAAT
ATTGAAAACTCTGACAAGGCCATCAAGCTATTAGAGCATTCTAAAGGAGCTGGTTCCAAA
ACTCTGCAACAAAATGCTGAAAGCAGATTCAAT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 744
Start on reference sequence 109
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BAG2
Symbol Nomenclature : BAG2
SYNONYM : BAG-2; dJ417I1.2
Designation : BAG family molecular chaperone regulator 2|BAG-family molecular chaperone regulator-2|bcl-2-associated athanogene 2|dJ417I1.2 (BAG-family molecular chaperone regulator 2)
Full Nomenclature : BCL2-associated athanogene 2
GENEID : 9532
Locus Tag : RP3-496N17.2
GI : 117644833
GenBank Accession : AM393005
HGNC : 938
MIM : 603882
Target GenBank: AL050287

 

Reference Sequence Alignment

  

HsCD00081553      ATGGCTCAGGCGAAGATCAACGCTAAAGCCAACGAGGGGCGCTTCTGCCGCTCCTCCTCC
NM_004282.3       ATGGCTCAGGCGAAGATCAACGCTAAAGCCAACGAGGGGCGCTTCTGCCGCTCCTCCTCC
                  ************************************************************

HsCD00081553      ATGGCTGACCGCTCCAGCCGCCTGCTGGAGAGCCTGGACCAGCTGGAGCTCAGGGTTGAA
NM_004282.3       ATGGCTGACCGCTCCAGCCGCCTGCTGGAGAGCCTGGACCAGCTGGAGCTCAGGGTTGAA
                  ************************************************************

HsCD00081553      GCTTTGAGAGAAGCAGCAACTGCTGTTGAGCAAGAGAAAGAAATCCTTCTGGAAATGATC
NM_004282.3       GCTTTGAGAGAAGCAGCAACTGCTGTTGAGCAAGAGAAAGAAATCCTTCTGGAAATGATC
                  ************************************************************

HsCD00081553      CACAGTATCCAAAATAGCCAGGACATGAGGCAGATCAGTGACGGAGAAAGAGAAGAATTA
NM_004282.3       CACAGTATCCAAAATAGCCAGGACATGAGGCAGATCAGTGACGGAGAAAGAGAAGAATTA
                  ************************************************************

HsCD00081553      AATCTGACTGCAAACCGTTTGATGGGAAGAACTCTCACCGTTGAAGTGTCAGTAGAAACA
NM_004282.3       AATCTGACTGCAAACCGTTTGATGGGAAGAACTCTCACCGTTGAAGTGTCAGTAGAAACA
                  ************************************************************

HsCD00081553      ATTAGAAACCCCCAGCAGCAAGAATCCCTAAAGCATGCCACAAGGATTATTGATGAGGTG
NM_004282.3       ATTAGAAACCCCCAGCAGCAAGAATCCCTAAAGCATGCCACAAGGATTATTGATGAGGTG
                  ************************************************************

HsCD00081553      GTCAATAAGTTTCTGGATGATTTGGGAAATGCCAAGAGTCATTTAATGTCGCTCTACAGT
NM_004282.3       GTCAATAAGTTTCTGGATGATTTGGGAAATGCCAAGAGTCATTTAATGTCGCTCTACAGT
                  ************************************************************

HsCD00081553      GCATGTTCATCTGAGGTGCCACATGGGCCAGTTGATCAGAAGTTTCAATCCATAGTAATT
NM_004282.3       GCATGTTCATCTGAGGTGCCACATGGGCCAGTTGATCAGAAGTTTCAATCCATAGTAATT
                  ************************************************************

HsCD00081553      GGCTGTGCTCTTGAAGATCAGAAGAAAATTAAGAGAAGATTAGAGACTCTGCTTAGAAAT
NM_004282.3       GGCTGTGCTCTTGAAGATCAGAAGAAAATTAAGAGAAGATTAGAGACTCTGCTTAGAAAT
                  ************************************************************

HsCD00081553      ATTGAAAACTCTGACAAGGCCATCAAGCTATTAGAGCATTCTAAAGGAGCTGGTTCCAAA
NM_004282.3       ATTGAAAACTCTGACAAGGCCATCAAGCTATTAGAGCATTCTAAAGGAGCTGGTTCCAAA
                  ************************************************************

HsCD00081553      ACTCTGCAACAAAATGCTGAAAGCAGATTCAAT---
NM_004282.3       ACTCTGCAACAAAATGCTGAAAGCAGATTCAATTAG
                  *********************************   

Protein

SMART domain : BAG : BAG domains, present in regulator of Hsp70 proteins
UniProt : O95816
HPRD : 04859

Clone

Cloning Information : 266177
HIP Master Clone ID : 198275
IMAGE ID : 100001689
Original Clone ID : FLH266177.01L

Organism


TAX_ID : 9606
Species Specific ID: 9532

Genome

Chromosome : 6
Map Location : 6p12.1-p11.2

Reagents

Labome : BAG2-antibody