DNASU Plasmid Repository • 480.965.5697 | Email

PASD1 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  PASD1
Gene Name:  PAS domain containing 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266218.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00081594

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2319nts         Open reading frame : 1 to 2319
Reference Sequence Alignment

ATGAAGATGAGAGGGGAAAAGAGAAGAGACAAGGTCAATCCAAAAAGCTCTCAAAGGAAA
TTAAACTGGATTCCATCATTTCCTACCTATGATTACTTCAACCAAGTGACGCTACAGTTA
TTAGATGGCTTTATGATTACACTGAGCACAGATGGAGTGATCATTTGTGTGGCTGAAAAC
ATCTCTTCTCTTCTTGGACATTTACCAGCTGAGATTGTGGGCAAAAAATTATTAAGCCTT
CTGCCTGATGAAGAGAAAGATGAAGTCTACCAAAAGATTATTCTCAAATTTCCTTTACTA
AACTCAGAAACACATATTGAATTTTGCTGTCATTTAAAAAGAGGAAATGTCGAACATGGT
GATAGTTCTGCTTACGAAAACGTGAAATTTATTGTGAATGTAAGAGATATTTGTAATGAG
TTTCCTGTGGTCTTTAGTGGCTTGTTTTCCAGCCACCTCTGTGCTGACTTTGCTGCATGT
GTTCCTCAGGAGGATCGGCTTTATCTTGTGGGAAATGTTTGCATTCTCAGGACTCAGCTC
CTGCAGCAACTTTACACTTCAAAGGCAGTCAGTGATGAAGCTGTACTTACACAAGATTCA
GATGAGGAACCTTTTGTGGGAGAGCTCAGTAGCTCTCAAGGTCAAAGAGGACACACTAGC
ATGAAAGCCGTGTACGTTGAACCCGCTGCTGCTGCTGCTGCTGCTGCTATCTCAGACGAC
CAAATTGATATTGCAGAGGTTGAGCAGTATGGACCACAAGAAAACGTTCACATGTTTGTA
GATTCTGATTCAACTTATTGCTCCAGTACAGTTTTCCTGGATACTATGCCTGAATCTCCA
GCCTTATCCTTGCAAGACTTTCGAGGTGAGCCTGAGGTGAATCCATTGTACAGGGCAGAC
CCAGTGGACCTGGAGTTCTCGGTGGATCAGGTGGACTCAGTGGACCAGGAGGGCCCAATG
GACCAGCAGGACCCAGAGAACCCAGTTGCCCCGTTGGACCAGGCAGGCCTGATGGATCCA
GTGGATCCAGAGGACTCAGTGGACCTGGGGGCTGCTGGCGCAAGTGCTCAGCCATTACAG
CCATCATCACCAGTTGCATATGACATCATTAGCCAGGAACTGGAACTGATGAAGAAGTTG
AAGGAGCAGCTAGAAGAGAGGACTTGGTTGCTGCATGATGCCATCCAAAACCAGCAGAAT
GCATTGGAATTGATGATGGATCACCTTCAGAAGCAGCCAAACACATTACGCCACGTTGTC
ATTCCTGATCTCCAATCTTCGGAGGCAGTGCCCAAGAAACAACAGAAACAACACGCTGGG
CAAGTGAAGCGGCCTCTCCCACATCCCAAGGACGTCAAGTGTTTCTGTGGTTTATCTTTA
TCCAACTCTCTCAAAAACACTGGGGAGCTTCAGGAGCCTTGTGTTGCCTTCAACCAGCAG
CAACTGGTGCAGCAAGAACAACACCTGAAGGAGCAGCAGCGGCAGCTGCGGGAGCAGCTG
CAACAGCTGAGAGAGCAAAGGAAGGTGCAGAAGCAGAAGAAGATGCAGGAGAAGAAGAAG
CTGCAGGAGCAGAAAATGCAGGAGAAGAAGAAGCTGCAGGAGCAGAGGCGGCAAAAGAAG
AAGAAGCTACAGGAGCGGAAGAAGTGGCAGGGGCAGATGCTACAGAAAGAGCCAGAGGAG
GAGCAGCAGAAGCAGCAGCTGCAAGAGCAGCCACTGAAGCATAATGTCATCGTGGGGAAT
GAGAGGGTGCAGATATGCCTGCAAAACCCACGTGACGTATCTGTGCCCCTCTGCAATCAC
CCTGTTAGATTTTTACAGGCCCAACCCATTGTTCCTGTCCAGAGAGCAGCTGAACAACAG
CCCTCTGGCTTCTATCAAGATGAAAACTGTGGGCAACAGGAAGATGAGAGTCAAAGTTTT
TATCCTGAGGCGTATCAAGGGCCCCCCGTGAACCAGCTGCCATTGATAGATACCTCAAAC
TCTGAGGCAATTTCTTCTTCCAGCATTCCTCAGTTTCCCATAACTTCAGACTCAACCATA
AGCACCCTGGAGACCCCACAGGATTACATCCGGCTTTGGCAAGAGTTGTCTGATTCACTC
GGTCCTGTTGTCCAAGTGAACACTTGGTCTTGCGATGAGCAGGGCACCCTGCACGGCCAA
CCCACCTACCATCAGGTGCAAGTTTCTGAGGTAGGAGTCGAGGGACCTCCTGATCCACAG
GCTTTCCAAGGCCCTGCTGCATACCAGCCAGACCAGATGAGATCTGCGGAGCAGACCAGA
TTGATGCCTGCAGAGCAACGTGACTCAAATAAGCCGTGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2465
Start on reference sequence 144
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PASD1
Symbol Nomenclature : PASD1
SYNONYM : CT63; OXTES1
Designation : PAS domain-containing protein 1|cancer/testis antigen 63
Full Nomenclature : PAS domain containing 1
GENEID : 139135
GI : 117644885
GenBank Accession : AM393031
HGNC : 20686
Vega : OTTHUMG00000024169
Target GenBank: BC040301

 

Reference Sequence Alignment

  

HsCD00081594      ATGAAGATGAGAGGGGAAAAGAGAAGAGACAAGGTCAATCCAAAAAGCTCTCAAAGGAAA
NM_173493.2       ATGAAGATGAGAGGGGAAAAGAGAAGAGACAAAGTCAATCCAAAAAGCTCTCAAAGGAAA
                  ********************************.***************************

HsCD00081594      TTAAACTGGATTCCATCATTTCCTACCTATGATTACTTCAACCAAGTGACGCTACAGTTA
NM_173493.2       TTAAACTGGATTCCATCATTTCCTACCTATGATTACTTCAACCAAGTGACGCTACAGTTA
                  ************************************************************

HsCD00081594      TTAGATGGCTTTATGATTACACTGAGCACAGATGGAGTGATCATTTGTGTGGCTGAAAAC
NM_173493.2       TTAGATGGCTTTATGATTACACTGAGCACAGATGGAGTGATCATTTGTGTGGCTGAAAAC
                  ************************************************************

HsCD00081594      ATCTCTTCTCTTCTTGGACATTTACCAGCTGAGATTGTGGGCAAAAAATTATTAAGCCTT
NM_173493.2       ATCTCTTCTCTTCTTGGACATTTACCAGCTGAGATTGTGGGCAAAAAATTATTAAGCCTT
                  ************************************************************

HsCD00081594      CTGCCTGATGAAGAGAAAGATGAAGTCTACCAAAAGATTATTCTCAAATTTCCTTTACTA
NM_173493.2       CTGCCTGATGAAGAGAAAGATGAAGTCTACCAAAAGATTATTCTCAAATTTCCTTTACTA
                  ************************************************************

HsCD00081594      AACTCAGAAACACATATTGAATTTTGCTGTCATTTAAAAAGAGGAAATGTCGAACATGGT
NM_173493.2       AACTCAGAAACACATATTGAATTTTGCTGTCATTTAAAAAGAGGAAATGTCGAACATGGT
                  ************************************************************

HsCD00081594      GATAGTTCTGCTTACGAAAACGTGAAATTTATTGTGAATGTAAGAGATATTTGTAATGAG
NM_173493.2       GATAGTTCTGCTTACGAAAACGTGAAATTTATTGTGAATGTAAGAGATATTTGTAATGAG
                  ************************************************************

HsCD00081594      TTTCCTGTGGTCTTTAGTGGCTTGTTTTCCAGCCACCTCTGTGCTGACTTTGCTGCATGT
NM_173493.2       TTTCCTGTGGTCTTTAGTGGCTTGTTTTCCAGCCACCTCTGTGCTGACTTTGCTGCATGT
                  ************************************************************

HsCD00081594      GTTCCTCAGGAGGATCGGCTTTATCTTGTGGGAAATGTTTGCATTCTCAGGACTCAGCTC
NM_173493.2       GTTCCTCAGGAGGATCGGCTTTATCTTGTGGGAAATGTTTGCATTCTCAGGACTCAGCTC
                  ************************************************************

HsCD00081594      CTGCAGCAACTTTACACTTCAAAGGCAGTCAGTGATGAAGCTGTACTTACACAAGATTCA
NM_173493.2       CTGCAGCAACTTTACACTTCAAAGGCAGTCAGTGATGAAGCTGTACTTACACAAGATTCA
                  ************************************************************

HsCD00081594      GATGAGGAACCTTTTGTGGGAGAGCTCAGTAGCTCTCAAGGTCAAAGAGGACACACTAGC
NM_173493.2       GATGAGGAACCTTTTGTGGGAGAGCTCAGTAGCTCTCAAGGTCAAAGAGGACACACTAGC
                  ************************************************************

HsCD00081594      ATGAAAGCCGTGTACGTTGAACCCGCTGCTGCTGCTGCTGCTGCTGCTATCTCAGACGAC
NM_173493.2       ATGAAAGCCGTGTACGTTGAACCCGCTGCTGCTGCTGCTGCTGCTGCTATCTCAGACGAC
                  ************************************************************

HsCD00081594      CAAATTGATATTGCAGAGGTTGAGCAGTATGGACCACAAGAAAACGTTCACATGTTTGTA
NM_173493.2       CAAATTGATATTGCAGAGGTTGAGCAGTATGGACCACAAGAAAACGTTCACATGTTTGTA
                  ************************************************************

HsCD00081594      GATTCTGATTCAACTTATTGCTCCAGTACAGTTTTCCTGGATACTATGCCTGAATCTCCA
NM_173493.2       GATTCTGATTCAACTTATTGCTCCAGTACAGTTTTCCTGGATACTATGCCTGAATCTCCA
                  ************************************************************

HsCD00081594      GCCTTATCCTTGCAAGACTTTCGAGGTGAGCCTGAGGTGAATCCATTGTACAGGGCAGAC
NM_173493.2       GCCTTATCCTTGCAAGACTTTCGAGGTGAGCCTGAGGTGAATCCATTGTACAGGGCAGAC
                  ************************************************************

HsCD00081594      CCAGTGGACCTGGAGTTCTCGGTGGATCAGGTGGACTCAGTGGACCAGGAGGGCCCAATG
NM_173493.2       CCAGTGGACCTGGAGTTCTCGGTGGATCAGGTGGACTCAGTGGACCAGGAGGGCCCAATG
                  ************************************************************

HsCD00081594      GACCAGCAGGACCCAGAGAACCCAGTTGCCCCGTTGGACCAGGCAGGCCTGATGGATCCA
NM_173493.2       GACCAGCAGGACCCAGAGAACCCAGTTGCCCCGTTGGACCAGGCAGGCCTGATGGATCCA
                  ************************************************************

HsCD00081594      GTGGATCCAGAGGACTCAGTGGACCTGGGGGCTGCTGGCGCAAGTGCTCAGCCATTACAG
NM_173493.2       GTGGATCCAGAGGACTCAGTGGACCTGGGGGCTGCTGGCGCAAGTGCTCAGCCATTACAG
                  ************************************************************

HsCD00081594      CCATCATCACCAGTTGCATATGACATCATTAGCCAGGAACTGGAACTGATGAAGAAGTTG
NM_173493.2       CCATCATCACCAGTTGCATATGACATCATTAGCCAGGAACTGGAACTGATGAAGAAGTTG
                  ************************************************************

HsCD00081594      AAGGAGCAGCTAGAAGAGAGGACTTGGTTGCTGCATGATGCCATCCAAAACCAGCAGAAT
NM_173493.2       AAGGAGCAGCTAGAAGAGAGGACTTGGTTGCTGCATGATGCCATCCAAAACCAGCAGAAT
                  ************************************************************

HsCD00081594      GCATTGGAATTGATGATGGATCACCTTCAGAAGCAGCCAAACACATTACGCCACGTTGTC
NM_173493.2       GCATTGGAATTGATGATGGATCACCTTCAGAAGCAGCCAAACACATTACGCCACGTTGTC
                  ************************************************************

HsCD00081594      ATTCCTGATCTCCAATCTTCGGAGGCAGTGCCCAAGAAACAACAGAAACAACACGCTGGG
NM_173493.2       ATTCCTGATCTCCAATCTTCGGAGGCAGTGCCCAAGAAACAACAGAAACAACACGCTGGG
                  ************************************************************

HsCD00081594      CAAGTGAAGCGGCCTCTCCCACATCCCAAGGACGTCAAGTGTTTCTGTGGTTTATCTTTA
NM_173493.2       CAAGTGAAGCGGCCTCTCCCACATCCCAAGGACGTCAAGTGTTTCTGTGGTTTATCTTTA
                  ************************************************************

HsCD00081594      TCCAACTCTCTCAAAAACACTGGGGAGCTTCAGGAGCCTTGTGTTGCCTTCAACCAGCAG
NM_173493.2       TCCAACTCTCTCAAAAACACTGGGGAGCTTCAGGAGCCTTGTGTTGCCTTCAACCAGCAG
                  ************************************************************

HsCD00081594      CAACTGGTGCAGCAAGAACAACACCTGAAGGAGCAGCAGCGGCAGCTGCGGGAGCAGCTG
NM_173493.2       CAACTGGTGCAGCAAGAACAACACCTGAAGGAGCAGCAGCGGCAGCTGCGGGAGCAGCTG
                  ************************************************************

HsCD00081594      CAACAGCTGAGAGAGCAAAGGAAGGTGCAGAAGCAGAAGAAGATGCAGGAGAAGAAGAAG
NM_173493.2       CAACAGCTGAGAGAGCAAAGGAAGGTGCAGAAGCAGAAGAAGATGCAGGAGAAGAAGAAG
                  ************************************************************

HsCD00081594      CTGCAGGAGCAGAAAATGCAGGAGAAGAAGAAGCTGCAGGAGCAGAGGCGGCAAAAGAAG
NM_173493.2       CTGCAGGAGCAGAAAATGCAGGAGAAGAAGAAGCTGCAGGAGCAGAGGCGGCAAAAGAAG
                  ************************************************************

HsCD00081594      AAGAAGCTACAGGAGCGGAAGAAGTGGCAGGGGCAGATGCTACAGAAAGAGCCAGAGGAG
NM_173493.2       AAGAAGCTACAGGAGCGGAAGAAGTGGCAGGGGCAGATGCTACAGAAAGAGCCAGAGGAG
                  ************************************************************

HsCD00081594      GAGCAGCAGAAGCAGCAGCTGCAAGAGCAGCCACTGAAGCATAATGTCATCGTGGGGAAT
NM_173493.2       GAGCAGCAGAAGCAGCAGCTGCAAGAGCAGCCACTGAAGCATAATGTCATCGTGGGGAAT
                  ************************************************************

HsCD00081594      GAGAGGGTGCAGATATGCCTGCAAAACCCACGTGACGTATCTGTGCCCCTCTGCAATCAC
NM_173493.2       GAGAGGGTGCAGATATGCCTGCAAAACCCACGTGACGTATCTGTGCCCCTCTGCAATCAC
                  ************************************************************

HsCD00081594      CCTGTTAGATTTTTACAGGCCCAACCCATTGTTCCTGTCCAGAGAGCAGCTGAACAACAG
NM_173493.2       CCTGTTAGATTTTTACAGGCCCAACCCATTGTTCCTGTCCAGAGAGCAGCTGAACAACAG
                  ************************************************************

HsCD00081594      CCCTCTGGCTTCTATCAAGATGAAAACTGTGGGCAACAGGAAGATGAGAGTCAAAGTTTT
NM_173493.2       CCCTCTGGCTTCTATCAAGATGAAAACTGTGGGCAACAGGAAGATGAGAGTCAAAGTTTT
                  ************************************************************

HsCD00081594      TATCCTGAGGCGTATCAAGGGCCCCCCGTGAACCAGCTGCCATTGATAGATACCTCAAAC
NM_173493.2       TATCCTGAGGCGTATCAAGGGCCCCCCGTGAACCAGCTGCCATTGATAGATACCTCAAAC
                  ************************************************************

HsCD00081594      TCTGAGGCAATTTCTTCTTCCAGCATTCCTCAGTTTCCCATAACTTCAGACTCAACCATA
NM_173493.2       TCTGAGGCAATTTCTTCTTCCAGCATTCCTCAGTTTCCCATAACTTCAGACTCAACCATA
                  ************************************************************

HsCD00081594      AGCACCCTGGAGACCCCACAGGATTACATCCGGCTTTGGCAAGAGTTGTCTGATTCACTC
NM_173493.2       AGCACCCTGGAGACCCCACAGGATTACATCCGGCTTTGGCAAGAGTTGTCTGATTCACTC
                  ************************************************************

HsCD00081594      GGTCCTGTTGTCCAAGTGAACACTTGGTCTTGCGATGAGCAGGGCACCCTGCACGGCCAA
NM_173493.2       GGTCCTGTTGTCCAAGTGAACACTTGGTCTTGCGATGAGCAGGGCACCCTGCACGGCCAA
                  ************************************************************

HsCD00081594      CCCACCTACCATCAGGTGCAAGTTTCTGAGGTAGGAGTCGAGGGACCTCCTGATCCACAG
NM_173493.2       CCCACCTACCATCAGGTGCAAGTTTCTGAGGTAGGAGTCGAGGGACCTCCTGATCCACAG
                  ************************************************************

HsCD00081594      GCTTTCCAAGGCCCTGCTGCATACCAGCCAGACCAGATGAGATCTGCGGAGCAGACCAGA
NM_173493.2       GCTTTCCAAGGCCCTGCTGCATACCAGCCAGACCAGATGAGATCTGCGGAGCAGACCAGA
                  ************************************************************

HsCD00081594      TTGATGCCTGCAGAGCAACGTGACTCAAATAAGCCGTGC---
NM_173493.2       TTGATGCCTGCAGAGCAACGTGACTCAAATAAGCCGTGCTAA
                  ***************************************   

Protein

SMART domain : PAS : PAS domain
UniProt : Q8IV76
HPRD : 06613

Clone

Cloning Information : 266218
HIP Master Clone ID : 198316
IMAGE ID : 100002018
Original Clone ID : FLH266218.01L

Organism


TAX_ID : 9606
Species Specific ID: 139135

Genome

Chromosome : X
Map Location : Xq28
Ensembl : ENSG00000166049

Reagents

Labome : PASD1-antibody