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MGC3207 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MRI1
Gene Name:  methylthioribose-1-phosphate isomerase 1
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266645.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082021

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 966nts         Open reading frame : 1 to 966
Reference Sequence Alignment

ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
GTCCGGGAGAGACGTGAAACGGAGCTATGCGAGCATTGGGAAGAGCATACCAGGCAGAGG
GAACTGCCACTGCGAGGGCCCCTGGGCGGGACTGTGCTCGAGACCCGGCCCTACAACCAG
GGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAGATCCCCGCCACCCTTATCACC
GACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTGTCAGCTGTGGTCGTGGGAGCT
GACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTGGGCACCTACCAGCTGGCCATT
GTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCCCCCAGCTCTTCATGTGACCTC
CGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGACCGGGCCAGGAGCTGACCGAT
GTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTTTGGAATCCTGCCTTCGATGTC
ACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAACTGGGGGTCTTTGCCCCTGAG
GAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGGGATGGAACCCTAGATGGACCC
CAGATG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1026
Start on reference sequence 58
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MRI1
Symbol Nomenclature : MRI1
SYNONYM : MRDI; MTNA; Ypr118w
Designation : M1Pi|MTR-1-P isomerase|S-methyl-5-thioribose-1-phosphate isomerase 1|mediator of RhoA-dependent invasion|methylthioribose-1-phosphate isomerase|methylthioribose-1-phosphate isomerase homolog|translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Full Nomenclature : methylthioribose-1-phosphate isomerase 1
GENEID : 84245
Locus Tag : UNQ6390/PRO21135
GI : 117645271
GenBank Accession : AM393224
HGNC : 28469
MIM : 615105
Vega : OTTHUMG00000180867
Target GenBank: AL834276

 

Reference Sequence Alignment

  

HsCD00082021        ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
NM_032285.2         ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
NM_001031727.2      ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTG
                    ************************************************************

HsCD00082021        CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
NM_032285.2         CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
NM_001031727.2      CTGCCCAAGCAGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATC
                    ************************************************************

HsCD00082021        CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
NM_032285.2         CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
NM_001031727.2      CGCGCCATGAAGGTGCGGGGCGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCC
                    ************************************************************

HsCD00082021        GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
NM_032285.2         GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
NM_001031727.2      GTGGAGCTGCAGGCGGGCGCCGGGGGACCGGGACTCGCCGCGCTCGTGGCCTTCGTGCGC
                    ************************************************************

HsCD00082021        GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
NM_032285.2         GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
NM_001031727.2      GACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTGTCAACATGGCCCGCGCCGCC
                    ************************************************************

HsCD00082021        CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
NM_032285.2         CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
NM_001031727.2      CGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTACGGAAGAGGCG
                    ************************************************************

HsCD00082021        GTCCGGGAGAGACG-----------------------------------------TGAAA
NM_032285.2         GTCCGGGAGAGACG-----------------------------------------TGAAA
NM_001031727.2      GTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC
                    ************                                           : **.

HsCD00082021        CGGAGCTATGCGAGCATTGGGAAGAGCATACCAGGCAGAGGGAACTGCCACTGCGAGGGC
NM_032285.2         CGGAGCTATGCGAGCATTGGGAAGAGCATACCAGGCAGAGGGAACTGCCACTGCGAGGGC
NM_001031727.2      CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGC
                    **.***::** *..*.*:**... .**.:.* .   ... **.:   **.*:***. ***

HsCD00082021        CCCTGGGCGGGACTGTGCTCG---------------------------------------
NM_032285.2         CCCTGGGCGGGACTGTGCTCG---------------------------------------
NM_001031727.2      AAGGTGACTGTGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACA
                    ..   *.* * .***: * .                                        

HsCD00082021        ------------------------------------------------------------
NM_032285.2         ------------------------------------------------------------
NM_001031727.2      GCCCTAGGTGTGATTCGCTCACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACA
                                                                                

HsCD00082021        -AGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
NM_032285.2         -AGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
NM_001031727.2      GAGACCCGGCCCTACAACCAGGGAGCCCGGCTGACGGCCTTTGAGCTGGTCTATGAGCAG
                     ***********************************************************

HsCD00082021        ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
NM_032285.2         ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
NM_001031727.2      ATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTGCCATGGCCCATAGGGGCGTG
                    ************************************************************

HsCD00082021        TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
NM_032285.2         TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
NM_001031727.2      TCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGCCAACAAGGTG
                    ************************************************************

HsCD00082021        GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
NM_032285.2         GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
NM_001031727.2      GGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC
                    ************************************************************

HsCD00082021        CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
NM_032285.2         CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
NM_001031727.2      CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGA
                    ************************************************************

HsCD00082021        CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
NM_032285.2         CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
NM_001031727.2      CCGGGCCAGGAGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTT
                    ************************************************************

HsCD00082021        TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
NM_032285.2         TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
NM_001031727.2      TGGAATCCTGCCTTCGATGTCACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAA
                    ************************************************************

HsCD00082021        CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
NM_032285.2         CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
NM_001031727.2      CTGGGGGTCTTTGCCCCTGAGGAGCTCCGGACAGCCCTAACCACCACCATCTCTTCCAGG
                    ************************************************************

HsCD00082021        GATGGAACCCTAGATGGACCCCAGATG---
NM_032285.2         GATGGAACCCTAGATGGACCCCAGATGTAA
NM_001031727.2      GATGGAACCCTAGATGGACCCCAGATGTAA
                    ***************************   

Pathways

Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of polyamines
Reactome : Methionine salvage pathway
Reactome : Sulfur amino acid metabolism

Protein

UniProt : Q9BV20
HPRD : 14548

Clone

Cloning Information : 266645
HIP Master Clone ID : 198743
IMAGE ID : 100001969
Original Clone ID : FLH266645.01L

Organism


TAX_ID : 9606
Species Specific ID: 84245

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000037757

Reagents

Labome : MRI1-antibody