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ZNF366 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF366
Gene Name:  zinc finger protein 366
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266760.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082136

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2232nts         Open reading frame : 1 to 2232
Reference Sequence Alignment

ATGCAGAAGGAAATGAAGATGATCAAAGACGAGGATGTGCATTTCGACTTGGCTGTGAAG
AAGACCCCCTCCTTTCCCCACTGCCTGCAGCCAGTGGCTTCTCGGGGAAAGGCTCCCCAA
AGACACCCCTTCCCGGAAGCTCTCCGAGGGCCATTTTCCCAGTTTCGGTATGAACCTCCC
CCAGGAGACCTAGATGGGTTCCCCGGGGTCTTCGAAGGAGCAGGGTCTAGGAAACGGAAG
AGCATGCCCACAAAGATGCCCTATAACCACCCTGCAGAAGAAGTCACCCTCGCCCTCCAC
TCAGAGGAGAACAAAAACCACGGCCTTCCCAACCTCCCTTTGCTGTTCCCGCAGCCCCCG
CGCCCCAAGTATGACTCTCAGATGATCGACCTGTGCAACGTGGGCTTCCAATTCTACCGC
AGCCTGGAACACTTTGGGGGCAAGCCCGTCAAGCAGGAACCCATTAAGCCCAGCGCCGTG
TGGCCCCAGCCAACGCCCACTCCATTCCTGCCCACGCCCTACCCCTACTACCCCAAAGTC
CACCCGGGCCTCATGTTCCCCTTCTTCGTGCCCTCGTCCTCGCCCTTCCCCTTCAGCCGG
CACACCTTCCTGCCCAAGCAGCCCCCGGAACCTCTGCTGCCCCGGAAAGCCGAGCCCCAG
GAGAGCGAGGAGACCAAGCAGAAGGTGGAGAGGGTGGACGTGAACGTGCAGATCGATGAC
AGCTACTACGTGGACGTGGGCGGCTCGCAGAAGCGCTGGCAGTGCCCCACCTGCGAGAAG
TCCTACACCTCCAAGTACAACCTGGTCACCCACATCCTGGGCCACAGTGGGATCAAGCCG
CACGCGTGCACGCACTGCGGGAAGCTCTTCAAGCAGCTCAGCCACCTGCATACCCACATG
CTGACCCACCAGGGCACGCGGCCCCACAAGTGCCAGGTGTGCCACAAGGCCTTCACCCAG
ACCAGCCACCTGAAGCGCCACATGATGCAGCACAGCGAGGTGAAGCCGCACAACTGCCGC
GTGTGCGGCCGCGGCTTTGCCTACCCCAGCGAGCTCAAGGCCCACGAAGCCAAGCACGCC
AGTGGGCGCGAGAACATCTGTGTGGAGTGCGGCCTCGACTTCCCCACCTTGGCCCAGCTG
AAGAGACACCTCACCACGCACCGGGGCCCCATCCAGTACAACTGCTCCGAGTGCGACAAG
ACCTTCCAGTACCCGAGCCAGCTGCAGAACCACATGATGAAGCACAAGGACATCCGGCCC
TACATCTGCTCAGAGTGTGGCATGGAGTTTGTGCAGCCGCACCACCTCAAGCAGCACTCC
CTCACCCACAAGGGTGTGAAGGAGCATAAGTGTGGGATTTGTGGGCGGGAGTTCACCCTG
CTGGCCAACATGAAGCGACACGTGCTGATCCACACCAACATCCGCGCCTACCAGTGTCAC
CTCTGCTACAAGAGCTTCGTGCAGAAGCAGACCCTCAAGGCACACATGATCGTCCACTCT
GACGTGAAGCCTTTCAAATGCAAGCTTTGTGGGAAGGAATTCAACCGGATGCACAACCTG
ATGGGCCACATGCACCTGCACTCAGACAGCAAACCCTTCAAGTGCCTCTATTGCCCAAGC
AAATTCACCCTGAAGGGGAACCTGACACGCCACATGAAAGTCAAGCATGGAGTCATGGAG
CGGGGCCTTCATTCCCAAGGTCTGGGAAGGGGGAGAATCGCCCTGGCACAGACAGCCGGT
GTCCTGAGGAGTCTGGAGCAGGAGGAGCCCTTTGACCTCTCTCAGAAGCGCCGGGCCAAG
GTGCCGGTGTTCCAGTCAGACGGGGAGAGTGCCCAGGGCAGCCACTGCCACGAGGAGGAA
GAGGAGGATAACTGCTACGAGGTGGAGCCCTACAGCCCTGGCCTGGCCCCCCAGAGCCAG
CAGCTCTGCACACCCGAGGATCTGTCCACCAAGTCGGAGCACGCCCCCGAGGTGCTGGAG
GAAGCCTGCAAGGAGGAGAAGGAGGATGCATCCAAGGGAGAATGGGAGAAGAGGAGCAAG
GGTGACCTTGGGGCAGAGGGCGGCCAGGAGAGAGACTGTGCCGGCAGAGATGAGTGTCTC
AGTCTCAGGGCTTTTCAGAGTACCCGGCGGGGCCCCTCTTTTTCTGATTACTTATACTTC
AAGCACAGAGATGAGAGTTTGAAAGAATTACTGGAGAGGAAAATGGAAAAACAAGCAGTG
CTTTTAGGTATC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2311
Start on reference sequence 77
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF366
Symbol Nomenclature : ZNF366
SYNONYM : DCSCRIPT
Full Nomenclature : zinc finger protein 366
GENEID : 167465
GI : 117644835
GenBank Accession : AM393006
HGNC : 18316
MIM : 610159
Vega : OTTHUMG00000100965
Target GenBank: BX647592

 

Reference Sequence Alignment

  

HsCD00082136      ATGCAGAAGGAAATGAAGATGATCAAAGACGAGGATGTGCATTTCGACTTGGCTGTGAAG
NM_152625.1       ATGCAGAAGGAAATGAAGATGATCAAAGACGAGGATGTGCATTTCGACTTGGCTGTGAAG
                  ************************************************************

HsCD00082136      AAGACCCCCTCCTTTCCCCACTGCCTGCAGCCAGTGGCTTCTCGGGGAAAGGCTCCCCAA
NM_152625.1       AAGACCCCCTCCTTTCCCCACTGCCTGCAGCCAGTGGCTTCTCGGGGAAAGGCTCCCCAA
                  ************************************************************

HsCD00082136      AGACACCCCTTCCCGGAAGCTCTCCGAGGGCCATTTTCCCAGTTTCGGTATGAACCTCCC
NM_152625.1       AGACACCCCTTCCCGGAAGCTCTCCGAGGGCCATTTTCCCAGTTTCGGTATGAACCTCCC
                  ************************************************************

HsCD00082136      CCAGGAGACCTAGATGGGTTCCCCGGGGTCTTCGAAGGAGCAGGGTCTAGGAAACGGAAG
NM_152625.1       CCAGGAGACCTAGATGGGTTCCCCGGGGTCTTCGAAGGAGCAGGGTCTAGGAAACGGAAG
                  ************************************************************

HsCD00082136      AGCATGCCCACAAAGATGCCCTATAACCACCCTGCAGAAGAAGTCACCCTCGCCCTCCAC
NM_152625.1       AGCATGCCCACAAAGATGCCCTATAACCACCCTGCAGAAGAAGTCACCCTCGCCCTCCAC
                  ************************************************************

HsCD00082136      TCAGAGGAGAACAAAAACCACGGCCTTCCCAACCTCCCTTTGCTGTTCCCGCAGCCCCCG
NM_152625.1       TCAGAGGAGAACAAAAACCACGGCCTTCCCAACCTCCCTTTGCTGTTCCCGCAGCCCCCG
                  ************************************************************

HsCD00082136      CGCCCCAAGTATGACTCTCAGATGATCGACCTGTGCAACGTGGGCTTCCAATTCTACCGC
NM_152625.1       CGCCCCAAGTATGACTCTCAGATGATCGACCTGTGCAACGTGGGCTTCCAATTCTACCGC
                  ************************************************************

HsCD00082136      AGCCTGGAACACTTTGGGGGCAAGCCCGTCAAGCAGGAACCCATTAAGCCCAGCGCCGTG
NM_152625.1       AGCCTGGAACACTTTGGGGGCAAGCCCGTCAAGCAGGAACCCATTAAGCCCAGCGCCGTG
                  ************************************************************

HsCD00082136      TGGCCCCAGCCAACGCCCACTCCATTCCTGCCCACGCCCTACCCCTACTACCCCAAAGTC
NM_152625.1       TGGCCCCAGCCAACGCCCACTCCATTCCTGCCCACGCCCTACCCCTACTACCCCAAAGTC
                  ************************************************************

HsCD00082136      CACCCGGGCCTCATGTTCCCCTTCTTCGTGCCCTCGTCCTCGCCCTTCCCCTTCAGCCGG
NM_152625.1       CACCCGGGCCTCATGTTCCCCTTCTTCGTGCCCTCGTCCTCGCCCTTCCCCTTCAGCCGG
                  ************************************************************

HsCD00082136      CACACCTTCCTGCCCAAGCAGCCCCCGGAACCTCTGCTGCCCCGGAAAGCCGAGCCCCAG
NM_152625.1       CACACCTTCCTGCCCAAGCAGCCCCCGGAACCTCTGCTGCCCCGGAAAGCCGAACCCCAG
                  *****************************************************.******

HsCD00082136      GAGAGCGAGGAGACCAAGCAGAAGGTGGAGAGGGTGGACGTGAACGTGCAGATCGATGAC
NM_152625.1       GAGAGCGAGGAGACCAAGCAGAAGGTGGAGAGGGTGGACGTGAACGTGCAGATCGATGAC
                  ************************************************************

HsCD00082136      AGCTACTACGTGGACGTGGGCGGCTCGCAGAAGCGCTGGCAGTGCCCCACCTGCGAGAAG
NM_152625.1       AGCTACTACGTGGACGTGGGCGGCTCGCAGAAGCGCTGGCAGTGCCCCACCTGCGAGAAG
                  ************************************************************

HsCD00082136      TCCTACACCTCCAAGTACAACCTGGTCACCCACATCCTGGGCCACAGTGGGATCAAGCCG
NM_152625.1       TCCTACACCTCCAAGTACAACCTGGTCACCCACATCCTGGGCCACAGTGGGATCAAGCCG
                  ************************************************************

HsCD00082136      CACGCGTGCACGCACTGCGGGAAGCTCTTCAAGCAGCTCAGCCACCTGCATACCCACATG
NM_152625.1       CACGCGTGCACGCACTGCGGGAAGCTCTTCAAGCAGCTCAGCCACCTGCATACCCACATG
                  ************************************************************

HsCD00082136      CTGACCCACCAGGGCACGCGGCCCCACAAGTGCCAGGTGTGCCACAAGGCCTTCACCCAG
NM_152625.1       CTGACCCACCAGGGCACGCGGCCCCACAAGTGCCAGGTGTGCCACAAGGCCTTCACCCAG
                  ************************************************************

HsCD00082136      ACCAGCCACCTGAAGCGCCACATGATGCAGCACAGCGAGGTGAAGCCGCACAACTGCCGC
NM_152625.1       ACCAGCCACCTGAAGCGCCACATGATGCAGCACAGCGAGGTGAAGCCGCACAACTGCCGC
                  ************************************************************

HsCD00082136      GTGTGCGGCCGCGGCTTTGCCTACCCCAGCGAGCTCAAGGCCCACGAAGCCAAGCACGCC
NM_152625.1       GTGTGCGGCCGCGGCTTTGCCTACCCCAGCGAGCTCAAGGCCCACGAAGCCAAGCACGCC
                  ************************************************************

HsCD00082136      AGTGGGCGCGAGAACATCTGTGTGGAGTGCGGCCTCGACTTCCCCACCTTGGCCCAGCTG
NM_152625.1       AGTGGGCGCGAGAACATCTGTGTGGAGTGCGGCCTCGACTTCCCCACCTTGGCCCAGCTG
                  ************************************************************

HsCD00082136      AAGAGACACCTCACCACGCACCGGGGCCCCATCCAGTACAACTGCTCCGAGTGCGACAAG
NM_152625.1       AAGAGACACCTCACCACGCACCGGGGCCCCATCCAGTACAACTGCTCCGAGTGCGACAAG
                  ************************************************************

HsCD00082136      ACCTTCCAGTACCCGAGCCAGCTGCAGAACCACATGATGAAGCACAAGGACATCCGGCCC
NM_152625.1       ACCTTCCAGTACCCGAGCCAGTTGCAGAACCACATGATGAAGCACAAGGACATCCGGCCC
                  ********************* **************************************

HsCD00082136      TACATCTGCTCAGAGTGTGGCATGGAGTTTGTGCAGCCGCACCACCTCAAGCAGCACTCC
NM_152625.1       TACATCTGCTCAGAGTGTGGCATGGAGTTTGTGCAGCCGCACCACCTCAAGCAGCACTCC
                  ************************************************************

HsCD00082136      CTCACCCACAAGGGTGTGAAGGAGCATAAGTGTGGGATTTGTGGGCGGGAGTTCACCCTG
NM_152625.1       CTCACCCACAAGGGTGTGAAGGAGCATAAGTGTGGGATTTGTGGGCGGGAGTTCACCCTG
                  ************************************************************

HsCD00082136      CTGGCCAACATGAAGCGACACGTGCTGATCCACACCAACATCCGCGCCTACCAGTGTCAC
NM_152625.1       CTGGCCAACATGAAGCGACACGTGCTGATCCACACCAACATCCGCGCCTACCAGTGTCAC
                  ************************************************************

HsCD00082136      CTCTGCTACAAGAGCTTCGTGCAGAAGCAGACCCTCAAGGCACACATGATCGTCCACTCT
NM_152625.1       CTCTGCTACAAGAGCTTCGTGCAGAAGCAGACCCTCAAGGCACACATGATCGTCCACTCT
                  ************************************************************

HsCD00082136      GACGTGAAGCCTTTCAAATGCAAGCTTTGTGGGAAGGAATTCAACCGGATGCACAACCTG
NM_152625.1       GACGTGAAGCCTTTCAAATGCAAGCTTTGTGGGAAGGAATTCAACCGGATGCACAACCTG
                  ************************************************************

HsCD00082136      ATGGGCCACATGCACCTGCACTCAGACAGCAAACCCTTCAAGTGCCTCTATTGCCCAAGC
NM_152625.1       ATGGGCCACATGCACCTGCACTCAGACAGCAAACCCTTCAAGTGCCTCTATTGCCCAAGC
                  ************************************************************

HsCD00082136      AAATTCACCCTGAAGGGGAACCTGACACGCCACATGAAAGTCAAGCATGGAGTCATGGAG
NM_152625.1       AAATTCACCCTGAAGGGGAACCTGACACGCCACATGAAAGTCAAGCATGGAGTCATGGAG
                  ************************************************************

HsCD00082136      CGGGGCCTTCATTCCCAAGGTCTGGGAAGGGGGAGAATCGCCCTGGCACAGACAGCCGGT
NM_152625.1       CGGGGCCTTCATTCCCAAGGTCTGGGAAGGGGGAGAATCGCCCTGGCACAGACAGCCGGT
                  ************************************************************

HsCD00082136      GTCCTGAGGAGTCTGGAGCAGGAGGAGCCCTTTGACCTCTCTCAGAAGCGCCGGGCCAAG
NM_152625.1       GTCCTGAGGAGTCTGGAGCAGGAGGAGCCCTTTGACCTCTCTCAGAAGCGCCGGGCCAAG
                  ************************************************************

HsCD00082136      GTGCCGGTGTTCCAGTCAGACGGGGAGAGTGCCCAGGGCAGCCACTGCCACGAGGAGGAA
NM_152625.1       GTGCCGGTGTTCCAGTCAGACGGGGAGAGTGCCCAGGGCAGCCACTGCCACGAGGAGGAA
                  ************************************************************

HsCD00082136      GAGGAGGATAACTGCTACGAGGTGGAGCCCTACAGCCCTGGCCTGGCCCCCCAGAGCCAG
NM_152625.1       GAGGAGGATAACTGCTACGAGGTGGAGCCCTACAGCCCTGGCCTGGCCCCCCAGAGCCAG
                  ************************************************************

HsCD00082136      CAGCTCTGCACACCCGAGGATCTGTCCACCAAGTCGGAGCACGCCCCCGAGGTGCTGGAG
NM_152625.1       CAGCTCTGCACACCCGAGGATCTGTCCACCAAGTCGGAGCACGCCCCCGAGGTGCTGGAG
                  ************************************************************

HsCD00082136      GAAGCCTGCAAGGAGGAGAAGGAGGATGCATCCAAGGGAGAATGGGAGAAGAGGAGCAAG
NM_152625.1       GAAGCCTGCAAGGAGGAGAAGGAGGATGCATCCAAGGGAGAATGGGAGAAGAGGAGCAAG
                  ************************************************************

HsCD00082136      GGTGACCTTGGGGCAGAGGGCGGCCAGGAGAGAGACTGTGCCGGCAGAGATGAGTGTCTC
NM_152625.1       GGTGACCTTGGGGCAGAGGGCGGCCAGGAGAGAGACTGTGCCGGCAGAGATGAGTGTCTC
                  ************************************************************

HsCD00082136      AGTCTCAGGGCTTTTCAGAGTACCCGGCGGGGCCCCTCTTTTTCTGATTACTTATACTTC
NM_152625.1       AGTCTCAGGGCTTTTCAGAGTACCCGGCGGGGCCCCTCTTTTTCTGATTACTTATACTTC
                  ************************************************************

HsCD00082136      AAGCACAGAGATGAGAGTTTGAAAGAATTACTGGAGAGGAAAATGGAAAAACAAGCAGTG
NM_152625.1       AAGCACAGAGATGAGAGTTTGAAAGAATTACTGGAGAGGAAAATGGAAAAACAAGCAGTG
                  ************************************************************

HsCD00082136      CTTTTAGGTATC---
NM_152625.1       CTTTTAGGTATCTAA
                  ************   

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q5HYI9
UniProt : Q8N895
HPRD : 18329

Clone

Cloning Information : 266760
HIP Master Clone ID : 198858
IMAGE ID : 100002489
Original Clone ID : FLH266760.01L

Organism


TAX_ID : 9606
Species Specific ID: 167465

Genome

Chromosome : 5
Map Location : 5q13.2|5q13.2
Ensembl : ENSG00000178175

Reagents

Labome : ZNF366-antibody