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DNAJC7 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  DNAJC7
Gene Name:  DnaJ (Hsp40) homolog, subfamily C, member 7
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266771.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23359685
Title: Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome
Authors: The ORFeome Collaboration
HsCD00082147

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1482nts         Open reading frame : 1 to 1482
Reference Sequence Alignment

ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGAC
GACCAAGAGGCGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCC
AAGAAAGATTACAATGAAGCTTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAA
AATGCTAGCTATTATGGTAATCGAGCAGCCACCTTGATGATGCTTGGAAGGTTCCGGGAA
GCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACAGTTTTGTCCGGGGACATCTA
CGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCGCAGCTTCCAG
AGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAG
GTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATC
CTCAAGGCAGAATGTTTAGCAATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGT
GACATTCTACGAATGGATTCCACCAATGCAGATGCTCTGTATGTACGAGGTCTTTGCCTT
TATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCGTACAGGCTCTCAGGATGGCT
CCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAAAGCAAAGAAA
GAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGG
GGTACGGTTAATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCA
GTGAAGCTTGATGACACTTACATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGAC
ACAGAACAGTATGAAGAAGCAGTACGAGACTATGAAAAAGTATACCAGACAGAGAAAACA
AAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAACTGAGGAAGAGTAAGAGGAAA
GATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGATCAAGAAAGCT
TATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGAT
CCCAAGAAAAAGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATG
GGTGATTTTGATCCAAACAATATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGC
TTTGAAGCATCTGGTCCAGGGAATTTCTTTTTTCAATTTGGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1502
Start on reference sequence 21
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : DNAJC7
Symbol Nomenclature : DNAJC7
SYNONYM : DJ11; DJC7; TPR2; TTC2
Designation : TPR repeat protein 2|dnaJ homolog subfamily C member 7|tetratricopeptide repeat domain 2|tetratricopeptide repeat protein 2
Full Nomenclature : DnaJ (Hsp40) homolog, subfamily C, member 7
GENEID : 7266
GI : 117645841
GenBank Accession : AM393511
HGNC : 12392
MIM : 601964
Vega : OTTHUMG00000180862
Target GenBank: NM_003315

 

Reference Sequence Alignment

  

HsCD00082147      ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGAC
NM_003315.3       ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGAC
                  ************************************************************

HsCD00082147      GACCAAGAGGCGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCC
NM_003315.3       GACCAAGAGGCGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCC
                  ************************************************************

HsCD00082147      AAGAAAGATTACAATGAAGCTTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAA
NM_003315.3       AAGAAAGATTACAATGAAGCTTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAA
                  ************************************************************

HsCD00082147      AATGCTAGCTATTATGGTAATCGAGCAGCCACCTTGATGATGCTTGGAAGGTTCCGGGAA
NM_003315.3       AATGCTAGCTATTATGGTAATCGAGCAGCCACCTTGATGATGCTTGGAAGGTTCCGGGAA
                  ************************************************************

HsCD00082147      GCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACAGTTTTGTCCGGGGACATCTA
NM_003315.3       GCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACAGTTTTGTCCGGGGACATCTA
                  ************************************************************

HsCD00082147      CGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCGCAGCTTCCAG
NM_003315.3       CGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCGCAGCTTCCAG
                  ************************************************************

HsCD00082147      AGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
NM_003315.3       AGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
                  ************************************************************

HsCD00082147      GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAG
NM_003315.3       GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAG
                  ************************************************************

HsCD00082147      GTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATC
NM_003315.3       GTTGTTTTCTGCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATC
                  ************************************************************

HsCD00082147      CTCAAGGCAGAATGTTTAGCAATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGT
NM_003315.3       CTCAAGGCAGAATGTTTAGCAATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGT
                  ************************************************************

HsCD00082147      GACATTCTACGAATGGATTCCACCAATGCAGATGCTCTGTATGTACGAGGTCTTTGCCTT
NM_003315.3       GACATTCTACGAATGGATTCCACCAATGCAGATGCTCTGTATGTACGAGGTCTTTGCCTT
                  ************************************************************

HsCD00082147      TATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCGTACAGGCTCTCAGGATGGCT
NM_003315.3       TATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCGTACAGGCTCTCAGGATGGCT
                  ************************************************************

HsCD00082147      CCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAAAGCAAAGAAA
NM_003315.3       CCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAAAGCAAAGAAA
                  ************************************************************

HsCD00082147      GAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
NM_003315.3       GAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
                  ************************************************************

HsCD00082147      GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGG
NM_003315.3       GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGG
                  ************************************************************

HsCD00082147      GGTACGGTTAATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCA
NM_003315.3       GGTACGGTTAATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCA
                  ************************************************************

HsCD00082147      GTGAAGCTTGATGACACTTACATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGAC
NM_003315.3       GTGAAGCTTGATGACACTTACATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGAC
                  ************************************************************

HsCD00082147      ACAGAACAGTATGAAGAAGCAGTACGAGACTATGAAAAAGTATACCAGACAGAGAAAACA
NM_003315.3       ACAGAACAGTATGAAGAAGCAGTACGAGACTATGAAAAAGTATACCAGACAGAGAAAACA
                  ************************************************************

HsCD00082147      AAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAACTGAGGAAGAGTAAGAGGAAA
NM_003315.3       AAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAACTGAAGAAGAGTAAGAGGAAA
                  *******************************************.****************

HsCD00082147      GATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGATCAAGAAAGCT
NM_003315.3       GATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGATCAAGAAAGCT
                  ************************************************************

HsCD00082147      TATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
NM_003315.3       TATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
                  ************************************************************

HsCD00082147      CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGAT
NM_003315.3       CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGAT
                  ************************************************************

HsCD00082147      CCCAAGAAAAAGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATG
NM_003315.3       CCCAAGAAAAAGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATG
                  ************************************************************

HsCD00082147      GGTGATTTTGATCCAAACAATATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGC
NM_003315.3       GGTGATTTTGATCCAAACAATATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGC
                  ************************************************************

HsCD00082147      TTTGAAGCATCTGGTCCAGGGAATTTCTTTTTTCAATTTGGC---
NM_003315.3       TTTGAAGCATCTGGTCCAGGGAATTTCTTTTTTCAATTTGGCTAA
                  ******************************************   

Clone

Cloning Information : 266771
HIP Master Clone ID : 198869
IMAGE ID : 100002500
Original Clone ID : FLH266771.01L

Organism


TAX_ID : 9606
Species Specific ID: 7266

Genome

Chromosome : 17
Map Location : 17q11.2
Ensembl : ENSG00000168259

Reagents

Labome : DNAJC7-antibody