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Explanation of Terms


Gene: Gene Symbol:  PA5178
Gene Name:  LysM domain/BON superfamily protein
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH043831.01F
Keyword: None
Species: Pseudomonas aeruginosa
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
PaCD00008227

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 438nts         Open reading frame : 1 to 438

ATGGGTATTTTCGCGTTCGTGAAGGAAGCCGGTGAAAAGCTGTGGGATACGCTGACGGGG
CATGAGGCACAGGCGGCCGAGTCGCTGAAGGAGCACGTCGCCAAGGTCGGCCTGGGCAAC
CCGAACATCCAGGTTTCGGTGGAAGGCGACAAGGTCATCGCCTCGGGCGAGGTGGCCAGC
CAGGAGGAGAAAGAGAAGATCCTGCTGGCGCTGGGCAACGTCGCCGGCGTCTCCTCGGTG
GAAGACAAGATCACCGTGGCCCAGGCCGCTCCGGAGGCGCGCTTCGTCACCGTGAAGAAA
GGCGACACCCTGAGCGCCATCGCCAAGGCCGAATACGGCAATGCCAACGCCTACATGAAG
ATCTTCGAGGCCAACAAGCCGATGCTCAGCCATCCGGACAAGATCTACCCCGGCCAGGTC
CTGCGCATTCCCGAGGGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 438
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

 

Gene Symbol : PA5178
GENEID : 881721
Locus Tag : PA5178
GI : 49076341
GenBank Accession : AY657274
Pseudomonas aeruginosa (PA) number : PA5178
Target GenBank: NP_253865

 

Protein

SMART domain : LysM : Lysin motif
UniProt : Q9HU11

Clone

Cloning Information : 43831
HIP Master Clone ID : 38213
Original Clone ID : FLH043831.01F

Organism


TAX_ID : 208964
Species Specific ID: 881721

Reagents

Labome : PA5178-cDNA