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FBXO7 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FBXO7
Gene Name:  F-box protein 7
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266995.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 24100556
Title: Expression, purification and crystallization of the FP domain of the human F-box protein Fbxo7.
Authors: The ORFeome Collaboration
HsCD00082371

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1566nts         Open reading frame : 1 to 1566
Reference Sequence Alignment

ATGAGGCTGCGGGTGCGGCTTCTGAAGCGGACCTGGCCGCTGGAGGTGCCCGAGACGGAG
CCGACGCTGGGGCATTTGCGCTCGCACCTGAGGCAGTCCCTGCTGTGCACCTGGGGGTAC
AGTTCTAATACCCGATTTACAATTACATTGAACTACAAGGATCCCCTCACTGGAGATGAA
GAGACCTTGGCTTCATATGGGATTGTTTCTGGGGACTTGATATGTTTGATTCTTCAAGAT
GACATTCCAGCGCCTAATATACCTTCATCCACAGATTCAGAGCATTCTTCACTCCAGAAT
AATGAGCAACCCTCTTTGGCCACCAGCTCCAATCAGACTAGCATACAGGATGAACAACCA
AGTGATTCATTCCAAGGACAGGCAGCCCAGTCTGGTGTTTGGAATGACGACAGTATGTTA
GGGCCTAGTCAAAATTTTGAAGCTGAGTCAATTCAAGATAATGCGCATATGGCAGAGGGC
ACAGGTTTCTATCCCTCAGAACCCATGCTCTGTAGTGAATCGGTGGAAGGGCAAGTGCCA
CATTCATTAGAGACCTTGTATCAATCAGCTGACTGTTCTGATGCCAATGATGCCTTGATA
GTGTTGATACATCTTCTCATGTTGGAGTCAGGTTACATACCTCAGGGCACCGAAGCCAAA
GCACTGTCCATGCCGGAGAAGTGGAAGTTGAGCGGGGTGTATAAGCTGCAGTACATGCAT
CCTCTCTGCGAGGGCAGCTCCGCTACTCTCACCTGTGTGCCTTTGGGAAACCTGATTGTT
GTAAATGCTACACTAAAAATCAACAATGAGATTAGAAGTGTGAAAAGATTGCAGCTGCTA
CCAGAATCTTTTATTTGCAAAGAGAAACTAGGGGAAAATGTAGCCAACATATACAAAGAT
CTTCAGAAACTCTCTCGCCTCTTTAAAGACCAGCTGGTGTATCCTCTTTTGGCTTTTACC
CGACAAGCACTGAACCTACCAGATGTATTTGGGTTGGTCGTCCTCCCATTGGAACTGAAA
CTACGGATCTTCCGACTTCTGGATGTTCGTTCCGTCTTGTCTTTGTCTGCGGTTTGTCGT
GACCTCTTTACTGCTTCAAATGACCCACTCCTGTGGAGGTTTTTATATCTGCGTGATTTT
CGAGACAATACTGTCAGAGTTCAAGACACAGATTGGAAAGAACTGTACAGGAAGAGGCAC
ATACAAAGAAAAGAATCCCCGAAAGGGCGGTTTGTGATGCTCCTGCCATCGTCAACTCAC
ACCATTCCATTCTATCCCAACCCCTTGCACCCTAGGCCATTTCCTAGCTCCCGCCTTCCT
CCAGGAATTATCGGGGGTGAATATGACCAAAGACCAACACTTCCCTATGTTGGAGACCCA
ATCAGTTCACTCATTCCTGGTCCTGGGGAGACGCCCAGCCAGTTTCCTCCACTGAGACCA
CGCTTTGATCCAGTTGGCCCACTTCCAGGACCTAACCCCATCTTGCCAGGGCGAGGCGGC
CCCAATGACAGATTTCCCTTTAGACCCAGCAGGGGTCGGCCAACTGATGGCCGGCTGTCA
TTCATG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1849
Start on reference sequence 281
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FBXO7
Symbol Nomenclature : FBXO7
SYNONYM : FBX; FBX07; FBX7; PARK15; PKPS
Designation : F-box only protein 7
Full Nomenclature : F-box protein 7
GENEID : 25793
Locus Tag : LL22NC03-28H9.2
GI : 117644247
GenBank Accession : AM392740
HGNC : 13586
MIM : 605648
Vega : OTTHUMG00000030674
Target GenBank: BC008361

 

Reference Sequence Alignment

  

HsCD00082371      ATGAGGCTGCGGGTGCGGCTTCTGAAGCGGACCTGGCCGCTGGAGGTGCCCGAGACGGAG
NM_012179.3       ATGAGGCTGCGGGTGCGGCTTCTGAAGCGGACCTGGCCGCTGGAGGTGCCCGAGACGGAG
                  ************************************************************

HsCD00082371      CCGACGCTGGGGCATTTGCGCTCGCACCTGAGGCAGTCCCTGCTGTGCACCTGGGGGTAC
NM_012179.3       CCGACGCTGGGGCATTTGCGCTCGCACCTGAGGCAGTCCCTGCTGTGCACCTGGGGGTAC
                  ************************************************************

HsCD00082371      AGTTCTAATACCCGATTTACAATTACATTGAACTACAAGGATCCCCTCACTGGAGATGAA
NM_012179.3       AGTTCTAATACCCGATTTACAATTACATTGAACTACAAGGATCCCCTCACTGGAGATGAA
                  ************************************************************

HsCD00082371      GAGACCTTGGCTTCATATGGGATTGTTTCTGGGGACTTGATATGTTTGATTCTTCAAGAT
NM_012179.3       GAGACCTTGGCTTCATATGGGATTGTTTCTGGGGACTTGATATGTTTGATTCTTCAAGAT
                  ************************************************************

HsCD00082371      GACATTCCAGCGCCTAATATACCTTCATCCACAGATTCAGAGCATTCTTCACTCCAGAAT
NM_012179.3       GACATTCCAGCGCCTAATATACCTTCATCCACAGATTCAGAGCATTCTTCACTCCAGAAT
                  ************************************************************

HsCD00082371      AATGAGCAACCCTCTTTGGCCACCAGCTCCAATCAGACTAGCATACAGGATGAACAACCA
NM_012179.3       AATGAGCAACCCTCTTTGGCCACCAGCTCCAATCAGACTAGCATGCAGGATGAACAACCA
                  ********************************************.***************

HsCD00082371      AGTGATTCATTCCAAGGACAGGCAGCCCAGTCTGGTGTTTGGAATGACGACAGTATGTTA
NM_012179.3       AGTGATTCATTCCAAGGACAGGCAGCCCAGTCTGGTGTTTGGAATGACGACAGTATGTTA
                  ************************************************************

HsCD00082371      GGGCCTAGTCAAAATTTTGAAGCTGAGTCAATTCAAGATAATGCGCATATGGCAGAGGGC
NM_012179.3       GGGCCTAGTCAAAATTTTGAAGCTGAGTCAATTCAAGATAATGCGCATATGGCAGAGGGC
                  ************************************************************

HsCD00082371      ACAGGTTTCTATCCCTCAGAACCCATGCTCTGTAGTGAATCGGTGGAAGGGCAAGTGCCA
NM_012179.3       ACAGGTTTCTATCCCTCAGAACCCATGCTCTGTAGTGAATCGGTGGAAGGGCAAGTGCCA
                  ************************************************************

HsCD00082371      CATTCATTAGAGACCTTGTATCAATCAGCTGACTGTTCTGATGCCAATGATGCCTTGATA
NM_012179.3       CATTCATTAGAGACCTTGTATCAATCAGCTGACTGTTCTGATGCCAATGATGCCTTGATA
                  ************************************************************

HsCD00082371      GTGTTGATACATCTTCTCATGTTGGAGTCAGGTTACATACCTCAGGGCACCGAAGCCAAA
NM_012179.3       GTGTTGATACATCTTCTCATGTTGGAGTCAGGTTACATACCTCAGGGCACCGAAGCCAAA
                  ************************************************************

HsCD00082371      GCACTGTCCATGCCGGAGAAGTGGAAGTTGAGCGGGGTGTATAAGCTGCAGTACATGCAT
NM_012179.3       GCACTGTCCATGCCGGAGAAGTGGAAGTTGAGCGGGGTGTATAAGCTGCAGTACATGCAT
                  ************************************************************

HsCD00082371      CCTCTCTGCGAGGGCAGCTCCGCTACTCTCACCTGTGTGCCTTTGGGAAACCTGATTGTT
NM_012179.3       CCTCTCTGCGAGGGCAGCTCCGCTACTCTCACCTGTGTGCCTTTGGGAAACCTGATTGTT
                  ************************************************************

HsCD00082371      GTAAATGCTACACTAAAAATCAACAATGAGATTAGAAGTGTGAAAAGATTGCAGCTGCTA
NM_012179.3       GTAAATGCTACACTAAAAATCAACAATGAGATTAGAAGTGTGAAAAGATTGCAGCTGCTA
                  ************************************************************

HsCD00082371      CCAGAATCTTTTATTTGCAAAGAGAAACTAGGGGAAAATGTAGCCAACATATACAAAGAT
NM_012179.3       CCAGAATCTTTTATTTGCAAAGAGAAACTAGGGGAAAATGTAGCCAACATATACAAAGAT
                  ************************************************************

HsCD00082371      CTTCAGAAACTCTCTCGCCTCTTTAAAGACCAGCTGGTGTATCCTCTTTTGGCTTTTACC
NM_012179.3       CTTCAGAAACTCTCTCGCCTCTTTAAAGACCAGCTGGTGTATCCTCTTCTGGCTTTTACC
                  ************************************************ ***********

HsCD00082371      CGACAAGCACTGAACCTACCAGATGTATTTGGGTTGGTCGTCCTCCCATTGGAACTGAAA
NM_012179.3       CGACAAGCACTGAACCTACCAGATGTATTTGGGTTGGTCGTCCTCCCATTGGAACTGAAA
                  ************************************************************

HsCD00082371      CTACGGATCTTCCGACTTCTGGATGTTCGTTCCGTCTTGTCTTTGTCTGCGGTTTGTCGT
NM_012179.3       CTACGGATCTTCCGACTTCTGGATGTTCGTTCCGTCTTGTCTTTGTCTGCGGTTTGTCGT
                  ************************************************************

HsCD00082371      GACCTCTTTACTGCTTCAAATGACCCACTCCTGTGGAGGTTTTTATATCTGCGTGATTTT
NM_012179.3       GACCTCTTTACTGCTTCAAATGACCCACTCCTGTGGAGGTTTTTATATCTGCGTGATTTT
                  ************************************************************

HsCD00082371      CGAGACAATACTGTCAGAGTTCAAGACACAGATTGGAAAGAACTGTACAGGAAGAGGCAC
NM_012179.3       CGAGACAATACTGTCAGAGTTCAAGACACAGATTGGAAAGAACTGTACAGGAAGAGGCAC
                  ************************************************************

HsCD00082371      ATACAAAGAAAAGAATCCCCGAAAGGGCGGTTTGTGATGCTCCTGCCATCGTCAACTCAC
NM_012179.3       ATACAAAGAAAAGAATCCCCGAAAGGGCGGTTTGTGATGCTCCTGCCATCGTCAACTCAC
                  ************************************************************

HsCD00082371      ACCATTCCATTCTATCCCAACCCCTTGCACCCTAGGCCATTTCCTAGCTCCCGCCTTCCT
NM_012179.3       ACCATTCCATTCTATCCCAACCCCTTGCACCCTAGGCCATTTCCTAGCTCCCGCCTTCCT
                  ************************************************************

HsCD00082371      CCAGGAATTATCGGGGGTGAATATGACCAAAGACCAACACTTCCCTATGTTGGAGACCCA
NM_012179.3       CCAGGAATTATCGGGGGTGAATATGACCAAAGACCAACACTTCCCTATGTTGGAGACCCA
                  ************************************************************

HsCD00082371      ATCAGTTCACTCATTCCTGGTCCTGGGGAGACGCCCAGCCAGTTTCCTCCACTGAGACCA
NM_012179.3       ATCAGTTCACTCATTCCTGGTCCTGGGGAGACGCCCAGCCAGTTTCCTCCACTGAGACCA
                  ************************************************************

HsCD00082371      CGCTTTGATCCAGTTGGCCCACTTCCAGGACCTAACCCCATCTTGCCAGGGCGAGGCGGC
NM_012179.3       CGCTTTGATCCAGTTGGCCCACTTCCAGGACCTAACCCCATCTTGCCAGGGCGAGGCGGC
                  ************************************************************

HsCD00082371      CCCAATGACAGATTTCCCTTTAGACCCAGCAGGGGTCGGCCAACTGATGGCCGGCTGTCA
NM_012179.3       CCCAATGACAGATTTCCCTTTAGACCCAGCAGGGGTCGGCCAACTGATGGCCGGCTGTCA
                  ************************************************************

HsCD00082371      TTCATG---
NM_012179.3       TTCATGTGA
                  ******   

Protein

SMART domain : FBOX : A Receptor for Ubiquitination Targets
UniProt : Q9Y3I1
HPRD : 07292

Clone

Cloning Information : 266995
HIP Master Clone ID : 199093
IMAGE ID : 100002588
Original Clone ID : FLH266995.01L

Organism


TAX_ID : 9606
Species Specific ID: 25793

Genome

Chromosome : 22
Map Location : 22q12.3
Ensembl : ENSG00000100225

Reagents

Labome : FBXO7-antibody