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OPN1SW (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN1SW
Gene Name:  opsin 1 (cone pigments), short-wave-sensitive
Sequence             
Original Clone ID: FLH269601.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082822

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1047nts         Open reading frame : 1 to 1047
Reference Sequence Alignment

ATGAGAAAAATGTCGGAGGAAGAGTTTTATCTGTTCAAAAATATCTCTTCAGTGGGGCCG
TGGGATGGGCCTCAGTACCACATTGCCCCTGTCTGGGCCTTCTACCTCCAGGCAGCTTTC
ATGGGCACTGTCTTCCTTATAGGGTTCCCACTCAATGCCATGGTGCTGGTGGCCACACTG
CGCTACAAAAAGTTGCGGCAGCCCCTCAACTACATTCTGGTCAACGTGTCCTTCGGAGGC
TTCCTCCTCTGCATCTTCTCTGTCTTCCCTGTCTTCGTCGCCAGCTGTAACGGATACTTC
GTCTTCGGTCGCCATGTTTGTGCTTTGGAGGGCTTCCTGGGCACTGTAGCAGGTCTGGTT
ACAGGATGGTCACTGGCCTTCCTGGCCTTTGAGCGCTACATTGTCATCTGTAAGCCCTTC
GGCAACTTCCGCTTCAGCTCCAAGCATGCACTGACGGTGGTCCTGGCTACCTGGACCATT
GGTATTGGCGTCTCCATCCCACCCTTCTTTGGCTGGAGCCGGTTCATCCCTGAGGGCCTG
CAGTGTTCCTGTGGCCCTGACTGGTACACCGTGGGCACCAAATACCGCAGCGAGTCCTAT
ACGTGGTTCCTCTTCATCTTCTGCTTCATTGTGCCTCTCTCCCTCATCTGCTTCTCCTAC
ACTCAGCTGCTGAGGGCCCTGAAAGCTGTTGCAGCTCAGCAGCAGGAGTCAGCTACGACC
CAGAAGGCTGAACGGGAGGTGAGCCGCATGGTGGTTGTGATGGTAGGATCCTTCTGTGTC
TGCTACGTGCCCTACGCGGCCTTCGCCATGTACATGGTCAACAACCGTAACCATGGGCTG
GACTTACGGCTTGTCACCATTCCTTCATTCTTCTCCAAGAGTGCTTGCATCTACAATCCC
ATCATCTACTGCTTCATGAATAAGCAGTTCCAAGCTTGCATCATGAAGATGGTGTGTGGG
AAGGCCATGACAGATGAATCCGACACATGCAGCTCCCAGAAAACAGAAGTTTCTACTGTC
TCGTCTACCCAAGTTGGCCCCAACTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1054
Start on reference sequence 8
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN1SW
Symbol Nomenclature : OPN1SW
SYNONYM : BCP; BOP; CBT
Designation : blue cone photoreceptor pigment|blue-sensitive opsin|short-wave-sensitive opsin 1
Full Nomenclature : opsin 1 (cone pigments), short-wave-sensitive
GENEID : 611
GI : 162318801
GenBank Accession : BC156719
HGNC : 1012
MIM : 613522
Vega : OTTHUMG00000158311
Target GenBank: NM_001708

 

Reference Sequence Alignment

  

HsCD00082822      ATGAGAAAAATGTCGGAGGAAGAGTTTTATCTGTTCAAAAATATCTCTTCAGTGGGGCCG
NM_001708.2       ATGAGAAAAATGTCGGAGGAAGAGTTTTATCTGTTCAAAAATATCTCTTCAGTGGGGCCG
                  ************************************************************

HsCD00082822      TGGGATGGGCCTCAGTACCACATTGCCCCTGTCTGGGCCTTCTACCTCCAGGCAGCTTTC
NM_001708.2       TGGGATGGGCCTCAGTACCACATTGCCCCTGTCTGGGCCTTCTACCTCCAGGCAGCTTTC
                  ************************************************************

HsCD00082822      ATGGGCACTGTCTTCCTTATAGGGTTCCCACTCAATGCCATGGTGCTGGTGGCCACACTG
NM_001708.2       ATGGGCACTGTCTTCCTTATAGGGTTCCCACTCAATGCCATGGTGCTGGTGGCCACACTG
                  ************************************************************

HsCD00082822      CGCTACAAAAAGTTGCGGCAGCCCCTCAACTACATTCTGGTCAACGTGTCCTTCGGAGGC
NM_001708.2       CGCTACAAAAAGTTGCGGCAGCCCCTCAACTACATTCTGGTCAACGTGTCCTTCGGAGGC
                  ************************************************************

HsCD00082822      TTCCTCCTCTGCATCTTCTCTGTCTTCCCTGTCTTCGTCGCCAGCTGTAACGGATACTTC
NM_001708.2       TTCCTCCTCTGCATCTTCTCTGTCTTCCCTGTCTTCGTCGCCAGCTGTAACGGATACTTC
                  ************************************************************

HsCD00082822      GTCTTCGGTCGCCATGTTTGTGCTTTGGAGGGCTTCCTGGGCACTGTAGCAGGTCTGGTT
NM_001708.2       GTCTTCGGTCGCCATGTTTGTGCTTTGGAGGGCTTCCTGGGCACTGTAGCAGGTCTGGTT
                  ************************************************************

HsCD00082822      ACAGGATGGTCACTGGCCTTCCTGGCCTTTGAGCGCTACATTGTCATCTGTAAGCCCTTC
NM_001708.2       ACAGGATGGTCACTGGCCTTCCTGGCCTTTGAGCGCTACATTGTCATCTGTAAGCCCTTC
                  ************************************************************

HsCD00082822      GGCAACTTCCGCTTCAGCTCCAAGCATGCACTGACGGTGGTCCTGGCTACCTGGACCATT
NM_001708.2       GGCAACTTCCGCTTCAGCTCCAAGCATGCACTGACGGTGGTCCTGGCTACCTGGACCATT
                  ************************************************************

HsCD00082822      GGTATTGGCGTCTCCATCCCACCCTTCTTTGGCTGGAGCCGGTTCATCCCTGAGGGCCTG
NM_001708.2       GGTATTGGCGTCTCCATCCCACCCTTCTTTGGCTGGAGCCGGTTCATCCCTGAGGGCCTG
                  ************************************************************

HsCD00082822      CAGTGTTCCTGTGGCCCTGACTGGTACACCGTGGGCACCAAATACCGCAGCGAGTCCTAT
NM_001708.2       CAGTGTTCCTGTGGCCCTGACTGGTACACCGTGGGCACCAAATACCGCAGCGAGTCCTAT
                  ************************************************************

HsCD00082822      ACGTGGTTCCTCTTCATCTTCTGCTTCATTGTGCCTCTCTCCCTCATCTGCTTCTCCTAC
NM_001708.2       ACGTGGTTCCTCTTCATCTTCTGCTTCATTGTGCCTCTCTCCCTCATCTGCTTCTCCTAC
                  ************************************************************

HsCD00082822      ACTCAGCTGCTGAGGGCCCTGAAAGCTGTTGCAGCTCAGCAGCAGGAGTCAGCTACGACC
NM_001708.2       ACTCAGCTGCTGAGGGCCCTGAAAGCTGTTGCAGCTCAGCAGCAGGAGTCAGCTACGACC
                  ************************************************************

HsCD00082822      CAGAAGGCTGAACGGGAGGTGAGCCGCATGGTGGTTGTGATGGTAGGATCCTTCTGTGTC
NM_001708.2       CAGAAGGCTGAACGGGAGGTGAGCCGCATGGTGGTTGTGATGGTAGGATCCTTCTGTGTC
                  ************************************************************

HsCD00082822      TGCTACGTGCCCTACGCGGCCTTCGCCATGTACATGGTCAACAACCGTAACCATGGGCTG
NM_001708.2       TGCTACGTGCCCTACGCGGCCTTCGCCATGTACATGGTCAACAACCGTAACCATGGGCTG
                  ************************************************************

HsCD00082822      GACTTACGGCTTGTCACCATTCCTTCATTCTTCTCCAAGAGTGCTTGCATCTACAATCCC
NM_001708.2       GACTTACGGCTTGTCACCATTCCTTCATTCTTCTCCAAGAGTGCTTGCATCTACAATCCC
                  ************************************************************

HsCD00082822      ATCATCTACTGCTTCATGAATAAGCAGTTCCAAGCTTGCATCATGAAGATGGTGTGTGGG
NM_001708.2       ATCATCTACTGCTTCATGAATAAGCAGTTCCAAGCTTGCATCATGAAGATGGTGTGTGGG
                  ************************************************************

HsCD00082822      AAGGCCATGACAGATGAATCCGACACATGCAGCTCCCAGAAAACAGAAGTTTCTACTGTC
NM_001708.2       AAGGCCATGACAGATGAATCCGACACATGCAGCTCCCAGAAAACAGAAGTTTCTACTGTC
                  ************************************************************

HsCD00082822      TCGTCTACCCAAGTTGGCCCCAACTAA
NM_001708.2       TCGTCTACCCAAGTTGGCCCCAACTGA
                  *************************.*

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : Disease
Reactome : Diseases associated with visual transduction
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Retinoid cycle disease events
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : The retinoid cycle in cones (daylight vision)
Reactome : Visual phototransduction
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

UniProt : P03999
UniProt : Q0PJU0
HPRD : 01836

Clone

Cloning Information : 269601
HIP Master Clone ID : 201103
IMAGE ID : 100062210
Original Clone ID : FLH269601.01X

Organism


TAX_ID : 9606
Species Specific ID: 611

Genome

Chromosome : 7
Map Location : 7q32.1
Ensembl : ENSG00000128617

Reagents

Labome : OPN1SW-antibody