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ZNF620 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF620
Gene Name:  zinc finger protein 620
Sequence             
Original Clone ID: FLH269865.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082988

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1269nts         Open reading frame : 1 to 1269
Reference Sequence Alignment

ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
CTGGAGAATTATGCAAATGTGGCTTCCCTGGCATTCCCATTCACCACGCCTGTTCTGGTC
TCCCAGCTGGAGCAAGGGGAACTGCCATGGGGCCTCGATCCCTGGGAACCTATGGGCAGG
GAGGCTCTCAGAGGTATCTGTCCAGGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
CAAGCATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1418
Start on reference sequence 150
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF620
Symbol Nomenclature : ZNF620
Full Nomenclature : zinc finger protein 620
GENEID : 253639
GI : 157170327
GenBank Accession : BC153008
HGNC : 28742
Vega : OTTHUMG00000156044
Target GenBank: NM_175888

 

Reference Sequence Alignment

  

HsCD00082988        ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
XM_005265013.1      ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
XM_005265012.1      ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
XM_005265011.1      ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
NM_175888.3         ATGTTCCAGACCGCTTGGCGCCAGGAACCAGTGACCTTTGAGGATGTGGCTGTGTACTTC
                    ************************************************************

HsCD00082988        ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
XM_005265013.1      ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
XM_005265012.1      ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
XM_005265011.1      ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
NM_175888.3         ACCCAGAATGAATGGGCCAGCCTGGACTCTGTGCAGAGGGCCCTGTACAGGGAAGTGATG
                    ************************************************************

HsCD00082988        CTGGAGAATTATGCAAATGTGGCTTCCCTGGCATTCCCATTCACCACGCCTGTTCTGGTC
XM_005265013.1      CTGGAGAATTATGCAAATGTGGCTTCCCTG------------------------------
XM_005265012.1      CTGGAGAATTATGCAAATGTGGCTTCCCTGGCATTCCCATTCACCACGCCTGTTCTGGTC
XM_005265011.1      CTGGAGAATTATGCAAATGTGGCTTCCCTGGCATTCCCATTCACCACGCCTGTTCTGGTC
NM_175888.3         CTGGAGAATTATGCAAATGTGGCTTCCCTGGCATTCCCATTCACCACGCCTGTTCTGGTC
                    ******************************                              

HsCD00082988        TCCCAGCTGGAGCAAGGGGAACTGCCATGGGGCCTCGATCCCTGGGAACCTATGGGCAGG
XM_005265013.1      ------------------------------------------------------------
XM_005265012.1      TCCCAGCTGGAGCAAGGGGAACTGCCATGGGGCCTCGATCCCTGGGAACCTATGGGCAGG
XM_005265011.1      TCCCAGCTGGAGCAAGGGGAACTGCCATGGGGCCTCGATCCCTGGGAACCTATGGGCAGG
NM_175888.3         TCCCAGCTGGAGCAAGGGGAACTGCCATGGGGCCTCGATCCCTGGGAACCTATGGGCAGG
                                                                                

HsCD00082988        GAGGCTCTCAGAGGTATCTGTCCAGGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
XM_005265013.1      ------------------------GGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
XM_005265012.1      GAGGCTCTCAGAGGTATCTGTCCAGGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
XM_005265011.1      GAGGCTCTCAGAGGTATCTGTCCAGGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
NM_175888.3         GAGGCTCTCAGAGGTATCTGTCCAGGGGATGAGGCCAGAACTGAGAAGGAAGGATTAACT
                                            ************************************

HsCD00082988        CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
XM_005265013.1      CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
XM_005265012.1      CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
XM_005265011.1      CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
NM_175888.3         CCAAAGGATCATGTGTCCAAAGAAACAGAGTCCTTCAGACTGATGGTGGGGGGCCTGCCA
                    ************************************************************

HsCD00082988        GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
XM_005265013.1      GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
XM_005265012.1      GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
XM_005265011.1      GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
NM_175888.3         GGGAATGTTTCCCAGCACCTTGACTTTGGGAGCAGCCTAGAGCAGCCACAAGGTCATTGG
                    ************************************************************

HsCD00082988        ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
XM_005265013.1      ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
XM_005265012.1      ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
XM_005265011.1      ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
NM_175888.3         ATAATTAAGACAAAGTCAAAGAGGAGACATTTCACAGATACCTCAGCCAGGCACCATGAG
                    ************************************************************

HsCD00082988        GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
XM_005265013.1      GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
XM_005265012.1      GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
XM_005265011.1      GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
NM_175888.3         GCCTATGAGGTCAAGAATGGAGAGAAGTTTGAGAAATTAGGAAAAAATATTAGCGTCAGC
                    ************************************************************

HsCD00082988        ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
XM_005265013.1      ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
XM_005265012.1      ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
XM_005265011.1      ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
NM_175888.3         ACACAACTCACTACAAATCAGACAAATCCTAGTGGTCAGATATCTTATGAATGTGGACAA
                    ************************************************************

HsCD00082988        TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
XM_005265013.1      TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
XM_005265012.1      TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
XM_005265011.1      TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
NM_175888.3         TGTGGCAGATATTTCATTCAAATGGCAGACTTCCACCGACATGAGAAATGTCACACTGGT
                    ************************************************************

HsCD00082988        GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
XM_005265013.1      GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
XM_005265012.1      GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
XM_005265011.1      GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
NM_175888.3         GAAAAGTCTTTTGAATGCAAAGAATGTGGAAAATACTTCAGATATAACTCATTACTTATT
                    ************************************************************

HsCD00082988        CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
XM_005265013.1      CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
XM_005265012.1      CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
XM_005265011.1      CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
NM_175888.3         CGGCATCAGATAATTCACACTGGAAAGAAACCATTTAAATGTAAAGAATGTGGAAAAGGT
                    ************************************************************

HsCD00082988        TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
XM_005265013.1      TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
XM_005265012.1      TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
XM_005265011.1      TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
NM_175888.3         TTAAGTTCAGACACAGCCTTGATTCAGCATCAGAGAATCCACACTGGAGAAAAGCCCTAT
                    ************************************************************

HsCD00082988        GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
XM_005265013.1      GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
XM_005265012.1      GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
XM_005265011.1      GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
NM_175888.3         GAATGTAAGGAGTGCGGCAAGGCCTTCAGTAGCAGCTCTGTCTTCCTCCAGCACCAGAGG
                    ************************************************************

HsCD00082988        TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
XM_005265013.1      TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
XM_005265012.1      TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
XM_005265011.1      TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
NM_175888.3         TTCCACACTGGGGAGAAGCTCTATGAATGTAACGAATGTTGGAAAACTTTCAGTTGCAGC
                    ************************************************************

HsCD00082988        TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
XM_005265013.1      TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
XM_005265012.1      TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
XM_005265011.1      TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
NM_175888.3         TCAAGTTTCACTGTCCATCAGCGAATGCACACTGGGGAGAAACCTTATGAATGTAAAGAG
                    ************************************************************

HsCD00082988        TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
XM_005265013.1      TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
XM_005265012.1      TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
XM_005265011.1      TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
NM_175888.3         TGTGGAAAACGATTAAGCTCCAACACAGCCTTGACTCAGCATCAGCGAATTCACACTGGG
                    ************************************************************

HsCD00082988        GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
XM_005265013.1      GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
XM_005265012.1      GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
XM_005265011.1      GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
NM_175888.3         GAGAAGCCCTTTGAATGTAAGGAGTGTGGGAAGGCATTCAATCAGAAAATAACCCTGATT
                    ************************************************************

HsCD00082988        CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
XM_005265013.1      CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
XM_005265012.1      CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
XM_005265011.1      CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
NM_175888.3         CAGCACCAGCGAGTTCACACTGGCGAGAAACCTTATGAGTGTAAAGTGTGTGGTAAAACC
                    ************************************************************

HsCD00082988        TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
XM_005265013.1      TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
XM_005265012.1      TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
XM_005265011.1      TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
NM_175888.3         TTCAGCTGGTGTGGAAGATTCATTCTGCATCAGAAACTACACACTCAGAAGACACCTGTC
                    ************************************************************

HsCD00082988        CAAGCATAA
XM_005265013.1      CAAGCATAG
XM_005265012.1      CAAGCATAG
XM_005265011.1      CAAGCATAG
NM_175888.3         CAAGCATAG
                    ********.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q6ZNG0
HPRD : 15878

Clone

Cloning Information : 269865
HIP Master Clone ID : 201367
IMAGE ID : 100016316
Original Clone ID : FLH269865.01X

Organism


TAX_ID : 9606
Species Specific ID: 253639

Genome

Chromosome : 3
Map Location : 3p22.1
Ensembl : ENSG00000177842

Reagents

Labome : ZNF620-antibody