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ZNF654 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF654
Gene Name:  zinc finger protein 654
Sequence             
Original Clone ID: FLH269873.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082996

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1746nts         Open reading frame : 1 to 1746
Reference Sequence Alignment

ATGTTATGTAACAAGGAATTTTTAGGTGGTCACATTGTAAGGCATGCCCAGGCTCATCAG
AAAAAAGGCAGTTTTGCATGTGTAATATGTGGTAGGAAATTTAGAAACAGAGGACTTATG
CAGAAGCATTTGAAGAATCATGTTAAGAAGATACAGAGGCAGCAAATTGCTGCAGCTCAA
CAGGATGATCAGGAAGTCACTGCTTTGGAAGAAATAAATTGTTCTAGTTCTTCCATTTCA
TTTGAAAATGGGAATTCTGATAGTAAGGATTTGGAAGTGGAGACACTTACTGCTTCTAGT
GAAGGAAACAAAGAAGTCATCCCTGAGCATGTGGCTGAATTCATTGAAATTCCCATAAGT
GTACCAGAAGATGTTATTGAAAATGTTATTGAAAATGGCAGTCCTAATAATTCTTTAAAT
AATGTTTTCAAGCCTTTAACTGAATGTGGGGATGATTATGAGGAGGAAGAGGATGAAGAA
GGTGATTATGAAGAAGATGATTATGACCTGAATCAAGAAACTTCAGTAATTCATAAAATC
AATGGAACTGTGTGCCATCCAAAAGACATATATGCCACAGATCAAGAAGGAAACTTTAAG
TGTCCTGCTCTTGGTTGTGTCCGGATATTTAAAAGAATTGGGTTTCTAAATAAACATGCA
ATGACCGTACATCCAACCGATTTAAATGTGCGACAAACAGTAATGAAGTGGAGCAAAGGA
AAATGCAAATTTTGTCAAAGGCAATTTGAAGATTCTCAACATTTTATAGACCACCTTAAT
AGACATAGCTATCCAAATGTGTATTTTTGTTTGCATTTTAATTGCAACGAGTCGTTTAAG
CTGCCGTTCCAGCTTGCCCAGCACACAAAAAGTCACAGGATATTTCAGGCTCAGTGTAGT
TTTCCAGAATGCCATGAGCTTTTTGAAGATCTTCCTCTGCTGTATGAACATGAAGCTCAA
CACTATTTAAGTAAAACACCAGAGTCATCTGCACAACCAAGTGAAACAATTCTTTGGGAT
GTTCAGACAGACTCAAATCCTAATCAGGAAAAAGACTCATCTAGTAATGAGAAACAAACT
ATTAGTCTGCCAGTTTCTACTAGCAAATCAAGGAAAGAGTCTACAGAACCAAAGACATGT
ATAGAAAGTATGGAAAAGAAAACAGACAGTTTAGTTCAGAATGGAAACGAACGTTCTGAT
GACACTGTTTCAAATATAAGCTTGATAGACCAAAAGATGCCTGACATAGAGCCAAATTCT
GAAAATAATTGTAGTAGTAGTGATATAGTCAATGGACACAGTGAAATAGAGCAAACACCT
TTAGTTTCATCAGATCCTGCTTTGAAAATTGATACAAACAGAATCAGGACAGAAAATGGT
TCCATTTTGCCCAGTGTTGTACCACAAGAACACAACACCTTGCCAGTATCTCAGGCACCT
TCCAAACCAAATCTGACAAGTGAACATACTTCATATGGCTTAATTTTAACAAAACCATAC
GTCAGACCATTGCCTCCCAGTTACCTTGATGAACGGTATCTTAGTATGCCAAAACGCAGA
AAATTTCTGACTGATAGAGTAGATGCCTGTTCTGATCAAGATAACGTGTATAAAAAATCA
GTGAAAAGATTAAGATGTGGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAG
GCTCATCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAAGT
GCCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1945
Start on reference sequence 200
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF654
Symbol Nomenclature : ZNF654
Designation : melanoma-associated antigen
Full Nomenclature : zinc finger protein 654
GENEID : 55279
GI : 157170075
GenBank Accession : BC153004
HGNC : 25612
Vega : OTTHUMG00000159097
Target GenBank: NM_018293

 

Reference Sequence Alignment

  

HsCD00082996      ATGTTATGTAACAAGGAATTTTTAGGTGGTCACATTGTAAGGCATGCCCAGGCTCATCAG
NM_018293.2       ATGTTATGTAACAAGGAATTTTTAGGTGGTCACATTGTAAGGCATGCCCAGGCTCATCAG
                  ************************************************************

HsCD00082996      AAAAAAGGCAGTTTTGCATGTGTAATATGTGGTAGGAAATTTAGAAACAGAGGACTTATG
NM_018293.2       AAAAAAGGCAGTTTTGCATGTGTAATATGTGGTAGGAAATTTAGAAACAGAGGACTTATG
                  ************************************************************

HsCD00082996      CAGAAGCATTTGAAGAATCATGTTAAGAAGATACAGAGGCAGCAAATTGCTGCAGCTCAA
NM_018293.2       CAGAAGCATTTGAAGAATCATGTTAAGAAGATACAGAGGCAGCAAATTGCTGCAGCTCAA
                  ************************************************************

HsCD00082996      CAGGATGATCAGGAAGTCACTGCTTTGGAAGAAATAAATTGTTCTAGTTCTTCCATTTCA
NM_018293.2       CAGGATGATCAGGAAGTCACTGCTTTGGAAGAAATAAATTGTTCTAGTTCTTCCATTTCA
                  ************************************************************

HsCD00082996      TTTGAAAATGGGAATTCTGATAGTAAGGATTTGGAAGTGGAGACACTTACTGCTTCTAGT
NM_018293.2       TTTGAAAATGGGAATTCTGATAGTAAGGATTTGGAAGTGGAGACACTTACTGCTTCTAGT
                  ************************************************************

HsCD00082996      GAAGGAAACAAAGAAGTCATCCCTGAGCATGTGGCTGAATTCATTGAAATTCCCATAAGT
NM_018293.2       GAAGGAAACAAAGAAGTCATCCCTGAGCATGTGGCTGAATTCATTGAAATTCCCATAAGT
                  ************************************************************

HsCD00082996      GTACCAGAAGATGTTATTGAAAATGTTATTGAAAATGGCAGTCCTAATAATTCTTTAAAT
NM_018293.2       GTACCAGAAGATGTTATTGAAAATGTTATTGAAAATGGCAGTCCTAATAATTCTTTAAAT
                  ************************************************************

HsCD00082996      AATGTTTTCAAGCCTTTAACTGAATGTGGGGATGATTATGAGGAGGAAGAGGATGAAGAA
NM_018293.2       AATGTTTTCAAGCCTTTAACTGAATGTGGGGATGATTATGAGGAGGAAGAGGATGAAGAA
                  ************************************************************

HsCD00082996      GGTGATTATGAAGAAGATGATTATGACCTGAATCAAGAAACTTCAGTAATTCATAAAATC
NM_018293.2       GGTGATTATGAAGAAGATGATTATGACCTGAATCAAGAAACTTCAGTAATTCATAAAATC
                  ************************************************************

HsCD00082996      AATGGAACTGTGTGCCATCCAAAAGACATATATGCCACAGATCAAGAAGGAAACTTTAAG
NM_018293.2       AATGGAACTGTGTGCCATCCAAAAGACATATATGCCACAGATCAAGAAGGAAACTTTAAG
                  ************************************************************

HsCD00082996      TGTCCTGCTCTTGGTTGTGTCCGGATATTTAAAAGAATTGGGTTTCTAAATAAACATGCA
NM_018293.2       TGTCCTGCTCTTGGTTGTGTCCGGATATTTAAAAGAATTGGGTTTCTAAATAAACATGCA
                  ************************************************************

HsCD00082996      ATGACCGTACATCCAACCGATTTAAATGTGCGACAAACAGTAATGAAGTGGAGCAAAGGA
NM_018293.2       ATGACCGTACATCCAACCGATTTAAATGTGCGACAAACAGTAATGAAGTGGAGCAAAGGA
                  ************************************************************

HsCD00082996      AAATGCAAATTTTGTCAAAGGCAATTTGAAGATTCTCAACATTTTATAGACCACCTTAAT
NM_018293.2       AAATGCAAATTTTGTCAAAGGCAATTTGAAGATTCTCAACATTTTATAGACCACCTTAAT
                  ************************************************************

HsCD00082996      AGACATAGCTATCCAAATGTGTATTTTTGTTTGCATTTTAATTGCAACGAGTCGTTTAAG
NM_018293.2       AGACATAGCTATCCAAATGTGTATTTTTGTTTGCATTTTAATTGCAACGAGTCGTTTAAG
                  ************************************************************

HsCD00082996      CTGCCGTTCCAGCTTGCCCAGCACACAAAAAGTCACAGGATATTTCAGGCTCAGTGTAGT
NM_018293.2       CTGCCGTTCCAGCTTGCCCAGCACACAAAAAGTCACAGGATATTTCAGGCTCAGTGTAGT
                  ************************************************************

HsCD00082996      TTTCCAGAATGCCATGAGCTTTTTGAAGATCTTCCTCTGCTGTATGAACATGAAGCTCAA
NM_018293.2       TTTCCAGAATGCCATGAGCTTTTTGAAGATCTTCCTCTGCTGTATGAACATGAAGCTCAA
                  ************************************************************

HsCD00082996      CACTATTTAAGTAAAACACCAGAGTCATCTGCACAACCAAGTGAAACAATTCTTTGGGAT
NM_018293.2       CACTATTTAAGTAAAACACCAGAGTCATCTGCACAACCAAGTGAAACAATTCTTTGGGAT
                  ************************************************************

HsCD00082996      GTTCAGACAGACTCAAATCCTAATCAGGAAAAAGACTCATCTAGTAATGAGAAACAAACT
NM_018293.2       GTTCAGACAGACTCAAATCCTAATCAGGAAAAAGACTCATCTAGTAATGAGAAACAAACT
                  ************************************************************

HsCD00082996      ATTAGTCTGCCAGTTTCTACTAGCAAATCAAGGAAAGAGTCTACAGAACCAAAGACATGT
NM_018293.2       ATTAGTCTGCCAGTTTCTACTAGCAAATCAAGGAAAGAGTCTACAGAACCAAAGACATGT
                  ************************************************************

HsCD00082996      ATAGAAAGTATGGAAAAGAAAACAGACAGTTTAGTTCAGAATGGAAACGAACGTTCTGAT
NM_018293.2       ATAGAAAGTATGGAAAAGAAAACAGACAGTTTAGTTCAGAATGGAAACGAACGTTCTGAT
                  ************************************************************

HsCD00082996      GACACTGTTTCAAATATAAGCTTGATAGACCAAAAGATGCCTGACATAGAGCCAAATTCT
NM_018293.2       GACACTGTTTCAAATATAAGCTTGATAGACCAAAAGATGCCTGACATAGAGCCAAATTCT
                  ************************************************************

HsCD00082996      GAAAATAATTGTAGTAGTAGTGATATAGTCAATGGACACAGTGAAATAGAGCAAACACCT
NM_018293.2       GAAAATAATTGTAGTAGTAGTGATATAGTCAATGGACACAGTGAAATAGAGCAAACACCT
                  ************************************************************

HsCD00082996      TTAGTTTCATCAGATCCTGCTTTGAAAATTGATACAAACAGAATCAGGACAGAAAATGGT
NM_018293.2       TTAGTTTCATCAGATCCTGCTTTGAAAATTGATACAAACAGAATCAGGACAGAAAATGGT
                  ************************************************************

HsCD00082996      TCCATTTTGCCCAGTGTTGTACCACAAGAACACAACACCTTGCCAGTATCTCAGGCACCT
NM_018293.2       TCCATTTTGCCCAGTGTTGTACCACAAGAACACAACACCTTGCCAGTATCTCAGGCACCT
                  ************************************************************

HsCD00082996      TCCAAACCAAATCTGACAAGTGAACATACTTCATATGGCTTAATTTTAACAAAACCATAC
NM_018293.2       TCCAAACCAAATCTGACAAGTGAACATACTTCATATGGCTTAATTTTAACAAAACCATAC
                  ************************************************************

HsCD00082996      GTCAGACCATTGCCTCCCAGTTACCTTGATGAACGGTATCTTAGTATGCCAAAACGCAGA
NM_018293.2       GTCAGACCATTGCCTCCCAGTTACCTTGATGAACGGTATCTTAGTATGCCAAAACGCAGA
                  ************************************************************

HsCD00082996      AAATTTCTGACTGATAGAGTAGATGCCTGTTCTGATCAAGATAACGTGTATAAAAAATCA
NM_018293.2       AAATTTCTGACTGATAGAGTAGATGCCTGTTCTGATCAAGATAACGTGTATAAAAAATCA
                  ************************************************************

HsCD00082996      GTGAAAAGATTAAGATGTGGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAG
NM_018293.2       GTGAAAAGATTAAGATGTGGCAAATGCCTGACCACCTACTGTAATGCAGAAGCACTTGAG
                  ************************************************************

HsCD00082996      GCTCATCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAAGT
NM_018293.2       GCTCATCTTGCACAAAAGAAATGTCAGACACTCTTTGGATTTGATTCAGATGATGAAAGT
                  ************************************************************

HsCD00082996      GCCTAA
NM_018293.2       GCCTGA
                  ****.*

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8IZM8
HPRD : 07722

Clone

Cloning Information : 269873
HIP Master Clone ID : 201375
IMAGE ID : 100016303
Original Clone ID : FLH269873.01X

Organism


TAX_ID : 9606
Species Specific ID: 55279

Genome

Chromosome : 3
Map Location : 3p11.1
Ensembl : ENSG00000175105

Reagents

Labome : ZNF654-antibody