DNASU Plasmid Repository • 480.965.5697 | Email

GP5 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  GP5
Gene Name:  glycoprotein V (platelet)
Sequence             
Original Clone ID: FLH270042.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00083069

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1680nts         Open reading frame : 1 to 1680
Reference Sequence Alignment

ATGCTGAGGGGGACTCTACTGTGCGCGGTGCTCGGGCTTCTGCGCGCCCAGCCCTTCCCC
TGTCCGCCAGCTTGCAAGTGTGTCTTCCGGGACGCCGCGCAGTGCTCGGGGGGCGACGTG
GCGCGCATCTCCGCGCTAGGCCTGCCCACCAACCTCACGCACATCCTGCTCTTCGGAATG
GGCCGCGGCGTCCTGCAGAGCCAGAGCTTCAGCGGCATGACCGTCCTGCAGCGCCTCATG
ATCTCCGACAGCCACATTTCCGCCGTTGCCCCCGGCACCTTCAGTGACCTGATAAAACTG
AAAACCCTGAGGCTGTCGCGCAACAAAATCACGCATCTTCCAGGTGCGCTGCTGGATAAG
ATGGTGCTCCTGGAGCAGTTGTTTTTGGACCACAATGCGCTAAGGGGCATTGACCAAAAC
ATGTTTCAGAAACTGGTTAACCTGCAGGAGCTCGCTCTGAACCAGAATCAGCTCGATTTC
CTTCCTGCCAGTCTCTTCACGAATCTGGAGAACCTGAAGTTGTTGGATTTATCGGGAAAC
AACCTGACCCACCTGCCCAAGGGGTTGCTTGGAGCACAGGCTAAGCTCGAGAGACTTCTG
CTCCACTCGAACCGCCTTGTGTCTCTGGATTCGGGGCTGTTGAACAGCCTGGGCGCCCTG
ACGGAGCTGCAGTTCCACCGAAATCACATCCGTTCCATCGCACCCGGGGCCTTCGACCGG
CTCCCAAACCTCAGTTCTTTGACGCTTTCGAGAAACCACCTTGCGTTTCTCCCCTCTGCG
CTCTTTCTTCATTCGCACAATCTGACTCTGTTGACTCTGTTCGAGAACCCGCTGGCAGAG
CTCCCGGGGGTGCTCTTCGGGGAGATGGGGGGCCTGCAGGAGCTGTGGCTGAACCGCACC
CAGCTGCGCACCCTGCCCGCCGCCGCCTTCCGCAACCTGAGCCGCCTGCGGTACTTAGGG
GTGACTCTGAGCCCGCGGCTGAGCGCGCTTCCGCAGGGCGCCTTCCAGGGCCTTGGCGAG
CTCCAGGTGCTCGCCCTGCACTCCAACGGCCTGACCGCCCTCCCCGACGGCTTGCTGCGC
GGCCTCGGCAAGCTGCGCCAGGTGTCCCTGCGCCGCAACAGGCTGCGCGCCCTGCCCCGT
GCCCTCTTCCGCAATCTCAGCAGCCTGGAGAGCGTCCAGCTCGACCACAACCAGCTGGAG
ACCCTGCCTGGCGACGTGTTTGGGGCTCTGCCCCGGCTGACGGAGGTCCTGTTGGGGCAC
AACTCCTGGCGCTGCGACTGTGGCCTGGGGCCCTTCCTGGGGTGGCTGCGGCAGCACCTA
GGCCTCGTGGGCGGGGAAGAGCCCCCACGGTGCGCAGGCCCTGGGGCGCACGCCGGCCTG
CCGCTCTGGGCCCTGCCGGGGGGTGACGCGGAGTGCCCGGGCCCCCGGGGCCCGCCTCCC
CGCCCCGCTGCGGACAGCTCCTCGGAAGCCCCTGTCCACCCAGCCTTGGCTCCCAACAGC
TCAGAACCCTGGGTGTGGGCCCAGCCGGTGACCACGGGCAAAGGTCAAGATCATAGTCCG
TTCTGGGGGTTTTATTTTCTGCTTTTAGCTGTTCAGGCCATGATCACCGTGATCATCGTG
TTTGCTATGATTAAAATTGGCCAACTCTTTCGAAAATTAATCAGAGAGAGAGCCCTTGGG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1714
Start on reference sequence 32
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GP5
Symbol Nomenclature : GP5
SYNONYM : CD42d; GPV
Designation : glycoprotein 5|platelet glycoprotein V
Full Nomenclature : glycoprotein V (platelet)
GENEID : 2814
GI : 157170229
GenBank Accession : BC152810
HGNC : 4443
MIM : 173511
Target GenBank: NM_004488

 

Reference Sequence Alignment

  

HsCD00083069      ATGCTGAGGGGGACTCTACTGTGCGCGGTGCTCGGGCTTCTGCGCGCCCAGCCCTTCCCC
NM_004488.2       ATGCTGAGGGGGACTCTACTGTGCGCGGTGCTCGGGCTTCTGCGCGCCCAGCCCTTCCCC
                  ************************************************************

HsCD00083069      TGTCCGCCAGCTTGCAAGTGTGTCTTCCGGGACGCCGCGCAGTGCTCGGGGGGCGACGTG
NM_004488.2       TGTCCGCCAGCTTGCAAGTGTGTCTTCCGGGACGCCGCGCAGTGCTCGGGGGGCGACGTG
                  ************************************************************

HsCD00083069      GCGCGCATCTCCGCGCTAGGCCTGCCCACCAACCTCACGCACATCCTGCTCTTCGGAATG
NM_004488.2       GCGCGCATCTCCGCGCTAGGCCTGCCCACCAACCTCACGCACATCCTGCTCTTCGGAATG
                  ************************************************************

HsCD00083069      GGCCGCGGCGTCCTGCAGAGCCAGAGCTTCAGCGGCATGACCGTCCTGCAGCGCCTCATG
NM_004488.2       GGCCGCGGCGTCCTGCAGAGCCAGAGCTTCAGCGGCATGACCGTCCTGCAGCGCCTCATG
                  ************************************************************

HsCD00083069      ATCTCCGACAGCCACATTTCCGCCGTTGCCCCCGGCACCTTCAGTGACCTGATAAAACTG
NM_004488.2       ATCTCCGACAGCCACATTTCCGCCGTTGCCCCCGGCACCTTCAGTGACCTGATAAAACTG
                  ************************************************************

HsCD00083069      AAAACCCTGAGGCTGTCGCGCAACAAAATCACGCATCTTCCAGGTGCGCTGCTGGATAAG
NM_004488.2       AAAACCCTGAGGCTGTCGCGCAACAAAATCACGCATCTTCCAGGTGCGCTGCTGGATAAG
                  ************************************************************

HsCD00083069      ATGGTGCTCCTGGAGCAGTTGTTTTTGGACCACAATGCGCTAAGGGGCATTGACCAAAAC
NM_004488.2       ATGGTGCTCCTGGAGCAGTTGTTTTTGGACCACAATGCGCTAAGGGGCATTGACCAAAAC
                  ************************************************************

HsCD00083069      ATGTTTCAGAAACTGGTTAACCTGCAGGAGCTCGCTCTGAACCAGAATCAGCTCGATTTC
NM_004488.2       ATGTTTCAGAAACTGGTTAACCTGCAGGAGCTCGCTCTGAACCAGAATCAGCTCGATTTC
                  ************************************************************

HsCD00083069      CTTCCTGCCAGTCTCTTCACGAATCTGGAGAACCTGAAGTTGTTGGATTTATCGGGAAAC
NM_004488.2       CTTCCTGCCAGTCTCTTCACGAATCTGGAGAACCTGAAGTTGTTGGATTTATCGGGAAAC
                  ************************************************************

HsCD00083069      AACCTGACCCACCTGCCCAAGGGGTTGCTTGGAGCACAGGCTAAGCTCGAGAGACTTCTG
NM_004488.2       AACCTGACCCACCTGCCCAAGGGGTTGCTTGGAGCACAGGCTAAGCTCGAGAGACTTCTG
                  ************************************************************

HsCD00083069      CTCCACTCGAACCGCCTTGTGTCTCTGGATTCGGGGCTGTTGAACAGCCTGGGCGCCCTG
NM_004488.2       CTCCACTCGAACCGCCTTGTGTCTCTGGATTCGGGGCTGTTGAACAGCCTGGGCGCCCTG
                  ************************************************************

HsCD00083069      ACGGAGCTGCAGTTCCACCGAAATCACATCCGTTCCATCGCACCCGGGGCCTTCGACCGG
NM_004488.2       ACGGAGCTGCAGTTCCACCGAAATCACATCCGTTCCATCGCACCCGGGGCCTTCGACCGG
                  ************************************************************

HsCD00083069      CTCCCAAACCTCAGTTCTTTGACGCTTTCGAGAAACCACCTTGCGTTTCTCCCCTCTGCG
NM_004488.2       CTCCCAAACCTCAGTTCTTTGACGCTTTCGAGAAACCACCTTGCGTTTCTCCCCTCTGCG
                  ************************************************************

HsCD00083069      CTCTTTCTTCATTCGCACAATCTGACTCTGTTGACTCTGTTCGAGAACCCGCTGGCAGAG
NM_004488.2       CTCTTTCTTCATTCGCACAATCTGACTCTGTTGACTCTGTTCGAGAACCCGCTGGCAGAG
                  ************************************************************

HsCD00083069      CTCCCGGGGGTGCTCTTCGGGGAGATGGGGGGCCTGCAGGAGCTGTGGCTGAACCGCACC
NM_004488.2       CTCCCGGGGGTGCTCTTCGGGGAGATGGGGGGCCTGCAGGAGCTGTGGCTGAACCGCACC
                  ************************************************************

HsCD00083069      CAGCTGCGCACCCTGCCCGCCGCCGCCTTCCGCAACCTGAGCCGCCTGCGGTACTTAGGG
NM_004488.2       CAGCTGCGCACCCTGCCCGCCGCCGCCTTCCGCAACCTGAGCCGCCTGCGGTACTTAGGG
                  ************************************************************

HsCD00083069      GTGACTCTGAGCCCGCGGCTGAGCGCGCTTCCGCAGGGCGCCTTCCAGGGCCTTGGCGAG
NM_004488.2       GTGACTCTGAGCCCGCGGCTGAGCGCGCTTCCGCAGGGCGCCTTCCAGGGCCTTGGCGAG
                  ************************************************************

HsCD00083069      CTCCAGGTGCTCGCCCTGCACTCCAACGGCCTGACCGCCCTCCCCGACGGCTTGCTGCGC
NM_004488.2       CTCCAGGTGCTCGCCCTGCACTCCAACGGCCTGACCGCCCTCCCCGACGGCTTGCTGCGC
                  ************************************************************

HsCD00083069      GGCCTCGGCAAGCTGCGCCAGGTGTCCCTGCGCCGCAACAGGCTGCGCGCCCTGCCCCGT
NM_004488.2       GGCCTCGGCAAGCTGCGCCAGGTGTCCCTGCGCCGCAACAGGCTGCGCGCCCTGCCCCGT
                  ************************************************************

HsCD00083069      GCCCTCTTCCGCAATCTCAGCAGCCTGGAGAGCGTCCAGCTCGACCACAACCAGCTGGAG
NM_004488.2       GCCCTCTTCCGCAATCTCAGCAGCCTGGAGAGCGTCCAGCTCGACCACAACCAGCTGGAG
                  ************************************************************

HsCD00083069      ACCCTGCCTGGCGACGTGTTTGGGGCTCTGCCCCGGCTGACGGAGGTCCTGTTGGGGCAC
NM_004488.2       ACCCTGCCTGGCGACGTGTTTGGGGCTCTGCCCCGGCTGACGGAGGTCCTGTTGGGGCAC
                  ************************************************************

HsCD00083069      AACTCCTGGCGCTGCGACTGTGGCCTGGGGCCCTTCCTGGGGTGGCTGCGGCAGCACCTA
NM_004488.2       AACTCCTGGCGCTGCGACTGTGGCCTGGGGCCCTTCCTGGGGTGGCTGCGGCAGCACCTA
                  ************************************************************

HsCD00083069      GGCCTCGTGGGCGGGGAAGAGCCCCCACGGTGCGCAGGCCCTGGGGCGCACGCCGGCCTG
NM_004488.2       GGCCTCGTGGGCGGGGAAGAGCCCCCACGGTGCGCAGGCCCTGGGGCGCACGCCGGCCTG
                  ************************************************************

HsCD00083069      CCGCTCTGGGCCCTGCCGGGGGGTGACGCGGAGTGCCCGGGCCCCCGGGGCCCGCCTCCC
NM_004488.2       CCGCTCTGGGCCCTGCCGGGGGGTGACGCGGAGTGCCCGGGCCCCCGGGGCCCGCCTCCC
                  ************************************************************

HsCD00083069      CGCCCCGCTGCGGACAGCTCCTCGGAAGCCCCTGTCCACCCAGCCTTGGCTCCCAACAGC
NM_004488.2       CGCCCCGCTGCGGACAGCTCCTCGGAAGCCCCTGTCCACCCAGCCTTGGCTCCCAACAGC
                  ************************************************************

HsCD00083069      TCAGAACCCTGGGTGTGGGCCCAGCCGGTGACCACGGGCAAAGGTCAAGATCATAGTCCG
NM_004488.2       TCAGAACCCTGGGTGTGGGCCCAGCCGGTGACCACGGGCAAAGGTCAAGATCATAGTCCG
                  ************************************************************

HsCD00083069      TTCTGGGGGTTTTATTTTCTGCTTTTAGCTGTTCAGGCCATGATCACCGTGATCATCGTG
NM_004488.2       TTCTGGGGGTTTTATTTTCTGCTTTTAGCTGTTCAGGCCATGATCACCGTGATCATCGTG
                  ************************************************************

HsCD00083069      TTTGCTATGATTAAAATTGGCCAACTCTTTCGAAAATTAATCAGAGAGAGAGCCCTTGGG
NM_004488.2       TTTGCTATGATTAAAATTGGCCAACTCTTTCGAAAATTAATCAGAGAGAGAGCCCTTGGG
                  ************************************************************

HsCD00083069      ---
NM_004488.2       TAA
                     

Pathways

Panther : Blood coagulation
Reactome : Formation of Fibrin Clot (Clotting Cascade)
Reactome : GP1b-IX-V activation signalling
Reactome : Hemostasis
Reactome : Intrinsic Pathway
Reactome : Platelet Adhesion to exposed collagen
Reactome : Platelet Aggregation (Plug Formation)
Reactome : Platelet activation, signaling and aggregation

Clone

Cloning Information : 270042
HIP Master Clone ID : 201544
IMAGE ID : 100016057
Original Clone ID : FLH270042.01L

Organism


TAX_ID : 9606
Species Specific ID: 2814

Genome

Chromosome : 3
Map Location : 3q29
Ensembl : ENSG00000178732

Reagents

Labome : GP5-antibody