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Nalp2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nlrp2
Gene Name:  NLR family, pyrin domain containing 2
Sequence             
Original Clone ID: FLH270257.01X
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00083183

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3141nts         Open reading frame : 1 to 3141

ATGGAACATTTTGATCCCCTGGGTTTCAACCTAAAAGACGTCCTGAGAAAGCTGGACAAG
ACTGAGTTGAACAATTTCAAGAGAACATTGAGGAGCTGTTCTCTGCCAGATACAATGAAA
CAGATCAACAAGCTGACAATGGACCTGGCTAATGGAGCTCAGCTAGCAGAAATTCTCACA
GACCACTGCCCCAGTGCTTGGATAAAGAGAGTCACCGTCCAGATCTTAGAGGAAATAAAC
CGTGTAGATCTAGCTGAGTTAGTAGTAAAGCAAATCGAAGAAGCAGTATTAAAGGTACCT
GAGGAAAAGGTTTCTTCTAAACCAAGAGAACCCTCGGGGACACTAACTTTCCCATGGAAT
TTTGTTCAAGGAGCTAAAAGGCCAGAGGACAAGCAAAAAGAGGAATGGAAGACTAGATAC
ACAGCTAAATGGAAGCAGAACTTCTGGCCTAAATGCAATAAAGAAATCTATGTGGTTACC
GAGAGCTACAAGACACTCCTGGCATTGTGTAACCCAAAGATAGAAACCCCATTTGCTCAT
GCAATTGTGCTGCATGGCCCTCCAGGCTCTGGGAAAACCACAATGGCAAAGCAGCTAATG
TTAGAATGGTCAGAGAGTAAGCAGGCCCAGATTTTCTCATGTGCCTTCTACATCAGCTGC
AGGGAAGTAAATAATACTAAACCTTGCACTTTTGCCCACCTCCTCTCCATGGACAATCCC
TCCTGGAGGGACTGTGTGATTCGAGATCTAATCCTGGGAAAGGAATTCTTATTTGTGGTA
GATGGTTTTGATGAGCTGACATTCCCAGCAGGAGCTCTGATCCGTGACCTTTGTGGTGAC
TGGAACACAGTGAAGCCTGTAGAGGTCTTACTGGGCAGTTTGCTGAAGAGGAAGATGGCA
CCTCATGCCACCCTGCTGGTAACCACACGGACACAGTCTTTGCACCAAATCTTTGTCATG
ATGGATCAGCCACTACTAGTAGAAACTCTGGGCTTCTTGGAGCAAGAAAAGCAGGAGTAC
TTTCAGAAATACTTTGAAGATGAGGAGGGTGAGGAGGAGGATAAAGGTGAGGGAAAGGCA
CTTAGAGCTCTCAAGGAAGTGAGATGCAATGCTGATCTATACCAAATGGCCTCACTTCCT
ACTGCATGTGGGATATTCTGCCTCTGTCTGGAGCTAAGGATGAAGAAGGGGGAAGACCTG
TCTCTGACATGTCAGACATACACCTCTATGTTCCTGAACTTCCTGTGCGAAGTCTTCTCA
TCAGAAACCTGTGAGGACCATCTCAATGAGGAATTCCAAATCTTATTCAAGAAAATATGT
ATCCTGGCTGCCAATAGTCTCTTGGAACAGGTGCCCATACTCTGTGAAGAAGACTTCTTG
ACATTAAAATTGAACCTAAACAATTTGCATCCCATGGTGTGCAGGCACATCCTCTTTAAG
GACAGTTCCAGTACACACTGTCTCTCTTTCATCTGCCTCGGTATCCAGCAACTCCTGGCT
GCAATAATATTTGTTCAGGAGCTTGGACAGGAAAGCAAGGGTGTCTCTAAATATAGTATA
CAGAACATGCTTTCAAGGGAAGCAAGATTAAAAAACCCTGACCTGTCTGGGCTGCTGCCA
TTTGTATTTGGCCTCCTGAATGAGACTCGTATTCAAGAGTTGAAGACTACTTTTGGCTGC
CAAATATCAACTGAGGTCAAGAGAAAATTCCTGGAGTGTGAGTCAGGGGAAAATAAACCC
TTACTATTACTGATGAATATGCAAGAAATCTTGTCCTGTCTCTATGAATCTCAGGAGGAG
GGGTTTGTGAAGGAAGCCATGGTCCTCTTTGAGGACATCTCCTTGCATTTGAAAACCAGC
ACTGACCTCATTCATGCTTCATTCTGCCTCAAGAACTCTCAGAACTTGCAGACAATGTCC
CTGAAAGTAGAAAAGGCGGTCTTTCCAGAGAATGTTGCTGCACTAGAGTCCACAGCTAAG
CATCAAAGGTCCCCGGATGAACAACGCATGTTAACTTTCTGGACAGACTTTTGTGACACA
TTTAATTCAAATAAGAAACTGGTATTTCTAGACATCCATGAAAGCTTCCTCAACAGCTCT
GCACTGGAGATTCTTTGTGAAAAGCTGCCCTCTGCTTCCTGTTGTCTCCAGAAAGTGGTC
CTCAAAAATATTTCCCCAGATGATGCCTACGAGAAATTGTGCCTCATTTTTAATGGTTAT
AAAACCATCTCACATCTGATCCTGCAAGGTGGCAACCTGGACAGCATGCATCACTCACTG
TGTGAGGTTCTGAAAAATCCAGCCTGCAACCTGAAGTTTCTCAGCCTGGGATCTTGTTCT
ACTGCTGCTCAGAAATGGGATGACTTCTTCCCAGTCCTGAAAGTCAATCAATCCCTGATA
TTTCTGGATCTCACAGACAACAGTCTCTTGGACAAAAGTGCTAAGCTCCTATGTAATATT
TGGAAGGAACCAAAGTGCATACTGCAGAGGGTATCGTTGGAGAACTGTCAGCTCACTGAA
GCCTGCTGCAAGGACCTCTCTTCCGTTTTGATGGTCAGCCGAACTCTGACACACCTCAGC
CTGGCCAACAATAAACTTGGGGATAACGGAGTGAAAAACCTGTGTGAAAGCATAAGCTGC
ACGGAGTGCAATCTACAGACCCTGGTGCTGTGGTCCTGTAACATCACCAACGCTGGTTGC
CACTATCTCTCAAAGATGCTCAAACAAACACTGAGCCTGAAACACTTGGATCTGGGGCTA
AATCGCATCGGAACCAAAGGAGCAAAGTTTCTGTGTGAGGCTTTGAAGAACCCCAAGAGT
AAATTGAAAAGTCTGTGGCTGTGTGGATGCTCCATCACTCCACTGAACTGCCAAGACTTC
TCTGAGACTCTTAGAAGCAACAAGAGCCTGAACACTCTAGACCTGTCTCAGAACGTCCTG
GGAACTGATGCCATAAAGACATTCTGTGAAGCCTTGAAACTTCAGATTTGCCCCCTGCAA
ATGCTTAGATTGAAATTTGATGAAGCTAAACCTAGTATCCAGAACCTGATCCAAGAGATG
AAGGTGAGCCATCCACAACTGAGAATCAGCAGTGACCAAGTGGATCTTAAGAATCCTCCT
CTTCCTCATTTCATTTTTTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3215
Start on reference sequence 75
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nlrp2
Symbol Nomenclature : Nlrp2
SYNONYM : E330007A02Rik; NBS1; Nalp2; PAN1; PYPAF2
Designation : NACHT, leucine rich repeat and PYD containing 2
Full Nomenclature : NLR family, pyrin domain containing 2
GENEID : 232827
GI : 162319183
GenBank Accession : BC156633
Mouse Genome Informatics : 3041206
Vega : OTTMUSG00000037748
Target GenBank: NM_177690

 

Clone

Cloning Information : 270257
HIP Master Clone ID : 201759
IMAGE ID : 100062160
Original Clone ID : FLH270257.01X

Organism


TAX_ID : 10090
Species Specific ID: 232827

Genome

Chromosome : 7
Map Location : 7 A1|7
Ensembl : ENSMUSG00000035177

Reagents

Labome : Nlrp2-antibody