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Farp1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Farp1
Gene Name:  FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
Sequence             
Original Clone ID: FLH270290.01L
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00083216

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3144nts         Open reading frame : 1 to 3144

ATGGGAGAAATAGAGCAGAAGCCCACCCCAGCATCACGTCTGGGGGCCCCAGAGAACTCG
GGGATCAGCACCTTGGAACGAGGACAGAAACCACCGCCCACACCTTCAGGAAAACTTATG
ACTGTCAAAATTCAGATGCTGGACGACACCCAGGAGGCCTTTGAAGTTCCACAACGAGCG
CCAGGGAAGGTTCTGTTTGACGCCGTGTGCAACCACCTCAACCTTGTGGAGGGCGACTAC
TTCGGCCTGGAGTTCCCTGACCACAGGAAGATCGTGGTGTGGCTGGATCTCCTGAAGCCC
ATTGTGAAGCAGATTCGAAGACCGAAGCACGTCGTTGTTAAATTTGTTGTGAAATTCTTC
CCTCCCGACCACACACAGCTCCAGGAAGAACTTACCAGGTACCTGTTCGCTCTGCAGGTG
AAGCAGGACTTGGCTCAAGGCAGGCTGACGTGTAACGACACCAGTGCCGCTCTCTTGATC
TCACACATTGTACAGTCGGAGATTGGGGATTTCGATGAAGCTTTGGACAGAGAACACTTA
GCAAAAAATAAGTACGTACCTCAACAGGATGCGCTGGAAGACAGAATCATGGAGTTCCAC
CACAGCCACGTTGGACAAACCCCGGCAGAGTCAGATTTCCAGCTCCTAGAGGTGGCCCGT
CGGCTAGAGATGTACGGAATCCGGTTACACCCTGCCAAGGACAGGGAAGGCACCAAGATC
AACCTGGCTGTCGCCAACACTGGAATTCTAGTGTTTCAGGGCTTCACGAAGATCAATGCT
TTTAACTGGGCCAAGGTGCGGAAGCTGAGCTTCAAGCGGAAGCGCTTTCTTATCAAGCTC
AGACCTGACGTGAATAGCTCATACCAGGACACCTTGGAGTTCCTGATGGCCGGCCGAGAC
TTCTGCAAGTCTTTCTGGAAAATATGTGTGGAGCACCATGCCTTCTTCAGACTCTTCGAA
GAACCTAAGCCAAAGCCAAAGCCTGTTCTCTTCAGCCGAGGGTCGTCCTTCCGGTTTAGT
GGTCGGACTCAGAAGCAAGTTCTTGATTATGTTAAAGAAGGCGGCCACAAGAAGGTGCAG
TTTGAAAGAAAGCACAGCAAGATCCACTCCACCAGGAGCCTGGTCTCACAGCCCACAGCA
CCGAATTCAGAAGTGCCAAAACAGTCTCCACAGAGCGCAAGCCTTACGTTTGGAGAAGGT
ACCGAGTCTCCGGGTGGCCAGAGCTGCCAGCAAGCGAAGGAAACAAAAGCCTGTACCTTG
GAGTTGGGACCTCACCAAAGCCCCGCCCTCCCTAAGAGCCCTCCAGGGAGCAAAGCTGCA
GATGGAACCACCGTGGTGCCGCCTGAGGAAGAAGAGGAGGAAGAGGGTGGCAAGGACGGC
ATCCGGCCAAGTAACCCTCAGCCCCCGCAGCCAAGCACAGGCTCCCTGACTGGTAGCCCT
CACCTTTCAGAGCTGTCCATCAACTCACAGGGAGGAGCCGCCCCAGCCAACGTGACCCTG
TCTCCCAACCTGAGTCCTGACAACAAGCAGGCCTCTCCCCTGATCAGCCCGCTCCTGAAC
GACCAAGCGTGCCCAAGGACCGACGACGAAGAAGAGGGCCGGCGAAAGAGATTCCCAACC
GACAAGGCGTACTACATTGCTAAGGAAGTCTCCACCACAGAGCGCACGTACCTGAAGGAC
CTTGAGGTCATTGCTTCGTGGTTTCAGAGCACAGTCAGCAAAGAGGACTCCATGCCCGAA
GCCTTGAAGAGTCTCATATTCCCAAATTTTGAACCTTTGCACAAGTTCCATACCAATTTT
CTGAAGGAAATTGAGCAACGACTTGCCCTGTGGGAAGGCCGCTCAAATGCCCACGTCAGA
GGAGATTACCAAAGAATCGGGGACGTCATGCTGAAAAACATTCAGGGCATGAAGCACCTG
GCGGCCCACCTGTGGAAGCACAGTGAGGCCCTGGAGGCCCTGGAGACCTCCATCAAGGGC
TCGCGGCGGCTGGAACATTTCTGCCGAGACTTCGAGCTGCAGAAGGTGTGTTACCTGCCG
CTCAACACCTTCCTCCTGCGCCCGCTGCACCGGCTCATGCACTATAAGCATGTCCTGGAG
AGGCTGTGCAAGCACCACCCACCAAACCACGCCGACTTCAGGGACTGCAGAGCTGCGCTG
GCGGAGATCACAGAGATGGTGGCACAGCTGCATGGTACCATGATCAAGATGGAGAACTTC
CAGAAGCTGCATGAGCTCAAGAAAGATCTGATCGGCATTGACAATCTTGTGACCCCAGGA
AGGGAGTTCATCCGCCTGGGCAGCCTCAGCAAGCTCTCCGGGAAGGGGCTTCAGCAGCGC
ATGTTCTTCCTGTTCAACGATGTCTTGCTGTATACCAGCCGGGGACTGACGGCATCTAAT
CAGTTTAAAGTCCACGGACAGCTCCCACTCTATGGCATGACGATCGAGGAGAGTGAGGAG
GAGTGGGGTGTGCCCCACTGCCTGACCCTGCGGGGCCAGCGGCAATCTATCATCGTGGCT
GCCAGTTCCCGGTCAGAGATGGAAAAATGGATGGAGGACATCCAGATGGCCATTGACCTG
GCAGAGAAAAGCAATGGACCCACCCCCGAGCTCCTTGCCAGCAGCCCCCCTGACAACAAG
TCCCCGGATGAAGCCACAGCGGCTGACCAGGAGTCAGAGGATGACCTGAGTGCCTCTCGG
ACATCGCTGGAGCGCCAGGCCCCTCACCGTGGCAACACGATGGTGCACGTGTGCTGGCAT
CGCAGCACCAGTGTCTCCATGGTGGACTTCAGCATTGCTGTGGAGAACCAGCTCTCTGGG
AACTTGCTGAGAAAGTTCAAGAACAGCAATGGCTGGCAGAAGCTGTGGGTGGTATTCACC
AACTTCTGCCTGTTCTTCTACAAATCACACCAGGACAGTCACCCCCTTGCCAGCCTGCCT
CTGCTGGGCTACTCCCTCACCATCCCCTCTGAGTCTGAGAACATCCACAAGGACTATGTG
TTCAAGCTGCATTTCAAGTCCCACGTGTACTACTTCAGGGCTGAAAGCGAATACACATTT
GAAAGGTGGATGGAAGTGATCCGAAGTGCCACCAGCTCAGCCTCTCGGGCCCACATCTTG
AGTCACAAAGAATCTCACCTGTAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3522
Start on reference sequence 376
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Farp1
Symbol Nomenclature : Farp1
SYNONYM : AW228844; BC030329; Cdep
Designation : FERM, RhoGEF and pleckstrin domain-containing protein 1|FERMRhoGEF (Arhgef) and pleckstrin domain protein 1
Full Nomenclature : FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived)
GENEID : 223254
GI : 162319133
GenBank Accession : BC156364
Mouse Genome Informatics : 2446173
Vega : OTTMUSG00000026446
Target GenBank: NM_134082

 

Clone

Cloning Information : 270290
HIP Master Clone ID : 201792
IMAGE ID : 100061900
Original Clone ID : FLH270290.01L

Organism


TAX_ID : 10090
Species Specific ID: 223254

Genome

Chromosome : 14
Map Location : 14 E5|14
Ensembl : ENSMUSG00000025555

Reagents

Labome : Farp1-antibody