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C130076O07Rik (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Nrcam
Gene Name:  neuron-glia-CAM-related cell adhesion molecule
Sequence             
Original Clone ID: FLH270312.01L
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00083238

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3753nts         Open reading frame : 1 to 3753

ATGCCGAAGAAGAAGCACTTATCTGCAGGCGGAGTGCCCCTGATTCTCTTCCTGTGCCAG
ATGATCAGCGCGCTGGATGTTCCTCTCGATCTGGTACAACCTCCAACCATCACTCAACAG
TCACCAAAAGACTACATCATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAGGCCAAA
GGGAAACCTCCCCCAAGCTTTTCCTGGACTCGTAATGGAACTCACTTTGACATAGACAAG
GACCCTCTGGTCACCATGAAGCCTGGCTCAGGAACCCTTGTCATCAACATCATGAGTGAG
GGGAAGGCAGAGACCTATGAAGGAGTCTACCAGTGCACTGCAAGGAACGAGCGTGGAGCT
GCCGTCTCCAATAACATTGTTGTCCGCCCATCTAGGTCACCCTTGTGGACCAAGGAAAGA
CTTGAACCTATAGTACTCCAGAATGGGCAGTCATTAGTACTTCCATGTAGGCCTCCGATT
GGATTACCTCCGGCCATAATATTTTGGATGGATAATTCCTTTCAGAGACTTCCACAGAGT
GAGCGGGTTTCCCAAGGCCTAAATGGAGACCTTTACTTCTCCAATGTCCTCCCAGAGGAC
ACCCGTGAGGACTATATCTGCTATGCCAGATTTAATCACACTCAAACCATCCAGCAGAAG
CAGCCTATTTCTTTGAAGGTGATTTCAGTGGATGAATTGAATGACACTATAGCTGCTAAT
TTGAGTGACACTGAGTTTTATGGTGCTAAATCTAGTAAAGAGAGGCCCCCAACGTTTCTA
ACTCCAGAGGGCAATGAAAGCCACAAAGAGGAATTAAGAGGAAACGTGCTTTCGCTGGAG
TGCATTGCGGAAGGCCTACCTACTCCAATTATTTACTGGATCAAAGAAGACGGAATGCTT
CCCGCCAACCGGACATTTTATCGGAACTTTAAGAAAACCCTGCAGATCACTCATGTTTCC
GAAGCCGACTCTGGAAATTATCAGTGCATAGCAAAAAACGCATTAGGAGCCGTCCATCAC
ACCATTTCTGTCACTGTTAAAGCGGCTCCCTACTGGATCGTGGCACCTCAAAACCTCGTG
CTTTCCCCGGGAGAGAATGGGACCCTCATCTGCAGAGCTAATGGCAACCCGAAACCCAGA
ATTAGCTGGTTAACAAATGGAGTCCCAATAGAAATTGCTCTCGATGACCCCAGCAGAAAA
ATAGATGGCGATACCATTATATTTTCAAATGTTCAAGAAAGCTCAAGTGCGGTTTATCAG
TGCAATGCCTCTAACAAATATGGATATTTACTAGCAAATGCATTTGTAAATGTTCTCGCT
GAACCACCTCGGATTCTCACATCAGCAAACACACTGTACCAGGTCATTGCAAACAGGCCT
GCTTTGCTAGATTGTGCCTTCTTTGGATCTCCTATGCCTACCATTGAGTGGTTTAAAGGC
ACTAAAGGAAGCGCTCTTCATGAAGACATTTATGTTTTGCATGATAATGGAACATTAGAA
ATTCCCGTGGCCCAAAAGGATAGTACAGGGACGTATACTTGTGTCGCACGGAATAAACTA
GGGATGGCAAAGAATGAAGTTCACTTGGAAATCAAAGATCCAACCAGGATCATTAAACAA
CCTGAGTATGCAGTCGTCCAGAGGGGGAGCAAGGTGTCCTTTGAATGCAGAGTGAAACAT
GACCACACCTTAATCCCCACCATTATGTGGCTGAAGGACAATGGAGAGCTGCCCAATGAT
GAAAGGTTCTCCACTGACAAGGATCATCTGGTGGTATCTGATGTAAAGGATGACGATGGC
GGAACCTACACGTGTACGGCCAACACAACGCTGGACAGTGCTTCGGCCAGCGCTGTGCTC
AGGGTTGTCGCTCCTACTCCAACTCCAGCCCCCATTTACGATGTCCCGAATCCTCCCTTT
GATTTAGAATTGACCAATCAACTTGACAAAAGTGTTCAGCTGACATGGACCCCAGGCGAC
GACAACAATAGCCCCATTACAAAATTCATCATTGAGTATGAAGATGCAATGCATGATGCA
GGGCTGTGGCGCCACCAGGCTGAAGTTTCTGGAACACAGACCACAGCCCAACTGAAGCTG
TCTCCCTATGTGAACTACTCCTTCCGTGTCATGGCAGAGAACAGCATTGGGAGAAGTATG
CCGAGCGAGGCATCCGAGCAGTATCTTACAAAAGCCGCAGAACCAGATCAGAATCCCATG
GCTGTGGAAGGACTAGGGACAGAGCCGGACAACTTGGTGATTACATGGAAGCCCCTGAAT
GGTTTTCAATCGAATGGGCCTGGCCTCCAGTACAAAGTGAGCTGGCGCCAGAAAGATGGT
GACGATGAGTGGACGTCTGTGGTTGTGGCCAATGTATCCAAATACATTGTTTCTGGCACA
CCAACCTTTGTCCCATACCTGATAAAAGTTCAAGCTCTGAATGATGTGGGGTTTGCACCA
GAGCCAGCTGCAGTCATGGGGCATTCTGGAGAAGACCTTCCAATGGTGGCTCCTGGAAAT
GTTCGCGTCAGCGTGGTGAACAGTACGCTGGCAGAGGTGCACTGGGACCCAGTTCCTCCC
AAGAGTGTCCGAGGACACTTACAAGGCTACCGGATTTACTACTGGAAGACCCAGAGCTCC
TCTAAAAGAAACAGGCGCCACATTGAGAAGAAGATCCTCACCTTCCAGGGCACCAAGACT
CACGGCATGCTGCCAGGGCTGCAGCCATACAGTCACTATGCCCTCAACGTCCGAGTGGTC
AACGGGAAAGGGGAGGGCCCAGCCAGCACGGACAGAGGCTTCCATACACCGGAGGGAGTC
CCTAGTGCTCCCTCATCTTTGAAAATTGTTAATCCCACACTGGACTCCCTCACTTTGGAA
TGGGACCCCCCAAGCCACCCAAATGGCATCCTGACTGAGTACATCTTACAATATCAGCCA
ATTAACAGCACACATGAGCTAGGCCCTCTGGTAGATTTAAAAATTCCTGCCAACAAGACC
CGCTGGACTTTGAAAAATTTAAATTTCAGCACTCGGTACAAGTTCTATTTCTATGCACAG
ACATCAGTGGGGCCAGGCAGTCAGATCACAGAGGAAGCGATAACGACTGTGGACGAAGCT
GGTATTCCTCCACCTGATGTAGGTGCAGGCAAAGGCAAAGAAGAATGGAGGAAAGAAATT
GTAAATGGTTCTCGAAGCTTCTTTGGGTTAAAGGGTCTAATGCCAGGAACAGCATACAAA
GTTCGAGTTGGTGCTGAGGGGGACTCTGGTTTTGTGAGTTCAGAGGATGTGTTTGAGACA
GGACCAGCAATGGCAAGCCGGCAAGTGGATATCGCGACCCAAGGCTGGTTCATAGGTCTA
ATGTGCGCTGTTGCTCTCCTCATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAAC
AAAGGTGGTAAATACCCAGTTAAAGAAAAGGAGGATGCTCATGCAGACCCTGAAATCCAG
CCCATGAAGGAAGATGATGGGACGTTTGGAGAATACAGTGATGCAGAAGATCACAAGCCT
TTGAAAAAAGGAAGTCGAACACCTTCAGACAGGACTGTGAAAAAAGAAGATAGCGATGAT
AGTCTGGTTGACTATGGAGAGGGGGTGAACGGCCAATTCAACGAGGATGGCTCCTTTATT
GGCCAATACAGTGGTAAGAAAGAGAAAGAGCCAGCAGAGGGAAATGAAAGCTCAGAGGCC
CCTTCTCCTGTCAACGCAATGAACTCCTTTGTT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4213
Start on reference sequence 458
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Nrcam
Symbol Nomenclature : Nrcam
SYNONYM : Bravo; C030017F07Rik; C130076O07Rik; mKIAA0343
Designation : mBravo|neuronal cell adhesion molecule|neuronal surface protein Bravo|ng-CAM-related|ngCAM-related cell adhesion molecule|nr-CAM
Full Nomenclature : neuron-glia-CAM-related cell adhesion molecule
GENEID : 319504
GI : 162319097
GenBank Accession : BC156235
Mouse Genome Informatics : 104750
Target GenBank: NM_176930

 

Protein

SMART domain : IGc2 : Immunoglobulin C-2 Type
SMART domain : FN3 : Fibronectin type 3 domain
SMART domain : IG : Immunoglobulin
UniProt : Q6QRP1
UniProt : Q810U4

Clone

Cloning Information : 270312
HIP Master Clone ID : 201814
IMAGE ID : 100061616
Original Clone ID : FLH270312.01L

Organism


TAX_ID : 10090
Species Specific ID: 319504

Genome

Chromosome : 12
Map Location : 12 B3|12 20.71 cM
Ensembl : ENSMUSG00000020598

Reagents

Labome : NrCAM-antibody